公开(公告)号：US20180135100A1

公开(公告)日：2018-05-17

申请号：US15818601

申请日：2017-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Lowe ,  Geoff McDermott ,  Christopher M. Hindson ,  Erin R. Chia ,  Amy L. Hiddessen ,  Benjamin J. Hindson ,  Chunxiao Han ,  Yaqi Wang

IPC: C12Q1/6804 ,  C12Q1/6844 ,  C12Q1/6851

Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：US10676778B2

公开(公告)日：2020-06-09

申请号：US15818601

申请日：2017-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Lowe ,  Geoff McDermott ,  Christopher M. Hindson ,  Erin R. Chia ,  Amy L. Hiddessen ,  Benjamin J. Hindson ,  Chunxiao Han ,  Yaqi Wang

IPC: C12Q1/68 ,  C12Q1/6804 ,  B01L7/00 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01L3/00

Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：US09822393B2

公开(公告)日：2017-11-21

申请号：US14201752

申请日：2014-03-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Lowe ,  Geoff McDermott ,  Christopher M. Hindson ,  Erin R. Chia ,  Amy L. Hiddessen ,  Benjamin J. Hindson ,  Chunxiao Han ,  Yaqi Wang

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

CPC classification number: C12Q1/6804 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q2537/143

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.