公开(公告)号：US12130285B2

公开(公告)日：2024-10-29

申请号：US18608025

申请日：2024-03-18

申请人： Slingshot Biosciences, Inc.

发明人： Keunho Ahn ,  Zhenyu Deng ,  Martina De Geus ,  Anh Tuan Nguyen

IPC分类号： G01N33/543 ,  G01N15/10 ,  G01N15/14

CPC分类号： G01N33/5436 ,  G01N15/1012 ,  G01N15/14 ,  G01N2015/1006

摘要： Provided herein are hydrogel beads that mimic live, dead, and apoptotic cells. The present disclosure also provides kits and compositions of hydrogel beads. The present disclosure further comprises methods of using the kits, compositions, and hydrogel beads to determine if a target cell sample includes one or more live, dead, or apoptotic cells.

公开(公告)号：US11965877B2

公开(公告)日：2024-04-23

申请号：US14874553

申请日：2015-10-05

申请人： Bio-Rad Laboratories, Inc.

发明人： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC分类号： G01N33/53 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20230251269A1

公开(公告)日：2023-08-10

申请号：US18015684

申请日：2021-07-15

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： Harry B. Larman ,  Daniel Monaco

IPC分类号： G01N33/68 ,  G01N33/543 ,  C12N15/10 ,  C12Q1/6844

CPC分类号： G01N33/6854 ,  G01N33/5436 ,  C12N15/1037 ,  C12Q1/6844 ,  G01N2800/24

摘要： The present invention relates to the field of allergies. More specifically, the present invention provides compositions and methods useful for identifying anti-allergen antibodies in a patient sample using phage display. In one embodiment, a method for detecting the presence of an antibody against an allergen in subject includes the steps of (a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library includes a plurality of peptides derived from a plurality of allergens; and (b) detecting at least one antibody bound to at least one peptide expressed by the display library, thereby detecting an antibody against the at least one peptide in the biological sample.

公开(公告)号：US11686726B2

公开(公告)日：2023-06-27

申请号：US16470607

申请日：2017-12-27

申请人： GE Healthcare Bio-Sciences AB

发明人： Ola Lind ,  Johan Fredrik Avallin ,  Andreas Torbjorn Lundin ,  Nils Norrman ,  Ronnie Palmgren ,  Gustav Jose Rodrigo

IPC分类号： G01N33/543 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12N13/00 ,  B01D21/00 ,  B03C5/00 ,  B03C5/02 ,  B03C1/30 ,  B03C1/28 ,  B03C1/01

CPC分类号： G01N33/54326 ,  B01D21/0009 ,  B03C1/30 ,  B03C5/00 ,  B03C5/02 ,  C12M23/48 ,  C12M27/16 ,  C12M29/00 ,  C12M41/44 ,  C12M45/07 ,  C12M47/04 ,  C12M47/10 ,  C12N13/00 ,  G01N33/5436 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  G01N2333/31

摘要： A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.

公开(公告)号：US20190218593A1

公开(公告)日：2019-07-18

申请号：US16215509

申请日：2018-12-10

申请人： Bio-Rad Laboratories, Inc.

发明人： Erin R. CHIA ,  Amy L. HIDDESSEN ,  Benjamin J. HINDSON ,  Adam LOWE ,  Chunxiao HAN

IPC分类号： C12Q1/686 ,  C08G77/46 ,  B01F17/54

CPC分类号： C12Q1/686 ,  B01F17/0071 ,  C07F7/1804 ,  C08G77/46 ,  C08G77/50 ,  C08G77/70 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

摘要： System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

公开(公告)号：US20180067108A1

公开(公告)日：2018-03-08

申请号：US15702938

申请日：2017-09-13

申请人： Universiteit Antwerpen

发明人： Ronny J.P. BLUST ,  Freddy DARDENNE ,  Karolien DE WAEL ,  Lucien NAGELS ,  Guido F.E. VAN CAMP

IPC分类号： G01N33/543 ,  G01N33/94 ,  G01N33/544 ,  G01N33/53 ,  G01N27/327

CPC分类号： G01N33/5438 ,  G01N27/3275 ,  G01N33/5308 ,  G01N33/5436 ,  G01N33/544 ,  G01N33/9413 ,  G01N33/9446 ,  G01N33/946 ,  G01N2333/78

摘要： This invention provides:—an aptamer-based electrochemical sensor, wherein said aptamer is covalently bonded to or chemisorbed on an electrode, said aptamer forming a complex with a target molecule and is encapsulated by a gelatin B matrix;—a method of manufacturing said aptamer-based electrochemical sensor;—the use of the aptamer-based electrochemical sensor for the electrochemical determination of a concentration of a target molecule; and—a composite electrode combining a polymeric material and electrically conducting particles for selective analyte detection, wherein said electrode is coated with gelatin type B.

公开(公告)号：US20180059108A1

公开(公告)日：2018-03-01

申请号：US15558300

申请日：2016-03-17

申请人： 3M INNOVATIVE PROPERTIES COMPANY

发明人： Manjiri T. Kshirsagar ,  Raj Rajagopal

IPC分类号： G01N33/569 ,  G01N21/76 ,  G01N33/543

CPC分类号： G01N33/569 ,  C12Q1/06 ,  G01N21/763 ,  G01N33/5436 ,  G01N33/54366 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N2469/00

摘要： Nonwoven articles for detecting microorganisms or cellular analytes in a fluid sample are provided. The nonwoven article includes a fibrous porous matrix and concentration agent particles enmeshed in the fibrous porous matrix. The fibrous porous matrix generally consists of inorganic fibers and polymeric fibers. Methods of detecting microorganisms or cellular analytes in a fluid sample are also provided. The method includes providing the nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article, and detecting the presence of bound microorganism strain(s) or bound cellular analyte(s).

公开(公告)号：US20180052154A1

公开(公告)日：2018-02-22

申请号：US15313366

申请日：2015-05-22

申请人： FIREFLY BIOWORKS, INC.

发明人： Isaac STONER ,  Timothy ERPS ,  Daniel PREGIBON ,  Jessica Dawn TYTELL ,  Andreas WINDEMUTH ,  Graeme DORAN

IPC分类号： G01N33/538 ,  G01N33/543

CPC分类号： G01N33/538 ,  G01N33/5432 ,  G01N33/5436

摘要： The present invention provides, among other things, methods, compositions and systems for highly efficient, robust, multiplex analysis of biomolecules based on substrate-mediated compartmentalization in conjunction with controlled release of as-say reagents for interference-free detection of biomolecules.

公开(公告)号：US20180045719A1

公开(公告)日：2018-02-15

申请号：US15559748

申请日：2016-03-23

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： G01N33/543 ,  G01N33/68 ,  C12N7/00 ,  C07K14/005 ,  C12N9/06

CPC分类号： G01N33/5436 ,  C07K14/005 ,  C07K2319/43 ,  C07K2319/735 ,  C12N7/00 ,  C12N9/003 ,  C12N2740/16023 ,  C12N2740/16042 ,  C12N2740/16222 ,  G01N33/54313 ,  G01N33/54353 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/6848 ,  G01N2333/161 ,  G01N2333/90666

摘要： This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

公开(公告)号：US20170138960A1

公开(公告)日：2017-05-18

申请号：US15115750

申请日：2015-02-02

申请人： DIADEXUS, INC.

发明人： Shaoqiu ZHUO

IPC分类号： G01N33/68 ,  G01N33/543 ,  A61K33/40 ,  G01N33/58

CPC分类号： G01N33/6893 ,  A61K33/40 ,  G01N33/54306 ,  G01N33/5436 ,  G01N33/581 ,  G01N2333/775 ,  G01N2333/918 ,  G01N2333/92 ,  G01N2800/324

摘要： Detection, monitoring and treatment of acute myocardial infarction (AMI) using a hybrid immunoassay to both Lp-PLA2 and ApoA1. Described herein are assays and method of performing them useful for the detection of AMI. Also described are methods of monitoring a patient at risk for, or suffering from, AMI. Also described herein are methods and assays for treating patient having or at risk of suffering from AMI.