公开(公告)号：US20200086312A1

公开(公告)日：2020-03-19

申请号：US16667811

申请日：2019-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. HIDDESSEN ,  Donald A. MASQUELIER ,  Kevin D. NESS ,  Benjamin J. HINDSON ,  Anthony J. MAKAREWICZ, JR. ,  Erin R. CHIA ,  Billy W. COLSTON, JR. ,  Serge SAXONOV ,  Svilen S. TZONEV ,  Michael Y. LUCERO ,  Ryan T. KOEHLER

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  B01F3/08 ,  B01F13/10 ,  C12Q1/686

Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.

公开(公告)号：US20190218593A1

公开(公告)日：2019-07-18

申请号：US16215509

申请日：2018-12-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin R. CHIA ,  Amy L. HIDDESSEN ,  Benjamin J. HINDSON ,  Adam LOWE ,  Chunxiao HAN

IPC: C12Q1/686 ,  C08G77/46 ,  B01F17/54

CPC classification number: C12Q1/686 ,  B01F17/0071 ,  C07F7/1804 ,  C08G77/46 ,  C08G77/50 ,  C08G77/70 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.