公开(公告)号：US20210002632A1

公开(公告)日：2021-01-07

申请号：US16935754

申请日：2020-07-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Serge SAXONOV

IPC: C12N15/10 ,  C12Q1/6876

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

公开(公告)号：US20220362764A1

公开(公告)日：2022-11-17

申请号：US17750195

申请日：2022-05-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. HINDSON ,  Serge SAXONOV ,  Phillip BELGRADER ,  Kevin D. NESS ,  Michael Y. LUCERO ,  Billy W. COLSTON, JR. ,  Shawn Paul HODGES ,  Nicholas J. HEREDIA ,  Jeffrey Clark MELLEN ,  Camille Bodley TROUP ,  Paul WYATT

IPC: B01L3/00 ,  B01L9/00 ,  C12Q1/686 ,  B01F23/41 ,  B01F33/81 ,  B01F33/3011

Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US20240271125A1

公开(公告)日：2024-08-15

申请号：US18584590

申请日：2024-02-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Serge SAXONOV

IPC: C12N15/10 ,  C12Q1/6876

CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1075 ,  C12Q1/6876

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

公开(公告)号：US20230287486A1

公开(公告)日：2023-09-14

申请号：US18055398

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. REGAN ,  Serge SAXONOV ,  Michael Y. LUCERO ,  Benjamin J. HINDSON ,  Phillip BELGRADER ,  Simant DUBE ,  Austin SO ,  Jeffrey Clark MELLEN ,  Nicholas Jack HEREDIA ,  Kevin D. NESS ,  Billy W. COLSTON, Jr.

IPC: C12Q1/6858 ,  C12Q1/683 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6858 ,  C12Q1/683 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2535/125

Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.

公开(公告)号：US20190211325A1

公开(公告)日：2019-07-11

申请号：US16223416

申请日：2018-12-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Serge SAXONOV

IPC: C12N15/10 ,  C12Q1/6876

CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1075 ,  C12Q1/6876 ,  C12Q2525/191 ,  C12Q2563/179

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

公开(公告)号：US20220355292A1

公开(公告)日：2022-11-10

申请号：US17750230

申请日：2022-05-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. HINDSON ,  Serge SAXONOV ,  Phillip BELGRADER ,  Kevin D. NESS ,  Michael Y. LUCERO ,  Billy W. COLSTON, JR.

IPC: B01L3/00 ,  B01L9/00 ,  C12Q1/686 ,  B01F23/41 ,  B01F33/81 ,  B01F33/3011

Abstract: This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US20200086312A1

公开(公告)日：2020-03-19

申请号：US16667811

申请日：2019-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. HIDDESSEN ,  Donald A. MASQUELIER ,  Kevin D. NESS ,  Benjamin J. HINDSON ,  Anthony J. MAKAREWICZ, JR. ,  Erin R. CHIA ,  Billy W. COLSTON, JR. ,  Serge SAXONOV ,  Svilen S. TZONEV ,  Michael Y. LUCERO ,  Ryan T. KOEHLER

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  B01F3/08 ,  B01F13/10 ,  C12Q1/686

Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.