公开(公告)号：US20200086312A1

公开(公告)日：2020-03-19

申请号：US16667811

申请日：2019-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. HIDDESSEN ,  Donald A. MASQUELIER ,  Kevin D. NESS ,  Benjamin J. HINDSON ,  Anthony J. MAKAREWICZ, JR. ,  Erin R. CHIA ,  Billy W. COLSTON, JR. ,  Serge SAXONOV ,  Svilen S. TZONEV ,  Michael Y. LUCERO ,  Ryan T. KOEHLER

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  B01F3/08 ,  B01F13/10 ,  C12Q1/686

Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.