公开(公告)号：US20180313213A1

公开(公告)日：2018-11-01

申请号：US16029683

申请日：2018-07-09

申请人： Schlumberger Technology Corporation

发明人： Farshid Mostowfi ,  Ronald E. G. van Hal ,  Shahnawaz Hossain Molla ,  Jane T. Lam ,  Amy Du ,  Neil William Bostrom ,  Michael Mallari Toribio

IPC分类号： E21B49/08 ,  B01L3/00 ,  B01F13/00

CPC分类号： E21B49/081 ,  B01F13/0072 ,  B01L3/502784 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0487 ,  E21B2049/085

摘要： A technique facilitates detection and analysis of constituents, e.g. chemicals, which may be found in formation fluids and/or other types of fluids. The technique comprises intermittently introducing a first fluid and a second fluid into a channel in a manner which forms slugs of the first fluid separated by the second fluid. The intermittent fluids are flowed through the channel to create a mixing action which mixes the fluid in the slugs. The mixing increases the exchange, e.g. transfer, of the chemical constituent between the second fluid and the first fluid. The exchange aids in sensing an amount of the chemical or chemicals for analysis. In many applications, the intermittent introduction, mixing, and measuring can be performed in a subterranean environment.

公开(公告)号：US20160296896A1

公开(公告)日：2016-10-13

申请号：US15094751

申请日：2016-04-08

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Richard T. Reel ,  David M. Cox ,  Sam L. Woo

IPC分类号： B01F3/08 ,  G01N1/38 ,  B01F13/00 ,  B01L3/00

CPC分类号： B01F3/0811 ,  B01F3/0815 ,  B01F5/0471 ,  B01F13/0071 ,  B01F13/0072 ,  B01F13/0076 ,  B01F2003/0842 ,  B01L3/0265 ,  B01L3/502769 ,  B01L3/502784 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0481 ,  B01L2400/0666 ,  G01N1/38 ,  G01N21/6428 ,  G01N35/08 ,  G01N2001/386 ,  G01N2021/6439 ,  G05D7/0694 ,  G05D11/131

摘要： Compositions are provided comprising a first fluid, in the form of discrete volumes, wherein the discrete volumes are spaced apart from one another by a second fluid that is immiscible with the first fluid. According to various embodiments, the composition can comprise a surface active agent, for example, a surfactant, that lowers, minimizes, and/or eliminates coalescence between two adjacent discrete volumes of the first fluid, in the absence of an applied electric potential or field. In some embodiments, the first fluid can comprise an aqueous solution, for example, containing biological material and/or reagents, and the second fluid can comprise an oil. Systems are also provided that can comprise a conduit system for flowing compositions such as those described above, and circuitry configured to supply an electric potential and/or electric field at, near, and/or adjacent, a junction in the conduit system. The electrical potential, and/or electric field generated thereby, can be matched to the coalescence properties of the composition such that, when applied, an addition fluid continuously flowed into a junction with the composition can be added directly to a discrete volume of the composition as it passes through the junction. Methods using the compositions and systems are also provided.

摘要翻译： 提供了包括离散体积形式的第一流体的组合物，其中离散体积通过与第一流体不混溶的第二流体彼此间隔开。 根据各种实施方案，组合物可以包含表面活性剂，例如表面活性剂，其在没有施加的电势或场的情况下降低，最小化和/或消除两个相邻离散体积的第一流体之间的聚结 。 在一些实施方案中，第一流体可以包含例如含有生物材料和/或试剂的水溶液，并且第二流体可以包含油。 还提供了系统，其可以包括用于流动诸如上述那些的组合物的管道系统，以及被配置为在管道系统中的接头处，附近和/或邻近处供应电势和/或电场的电路。 由此产生的电势和/或电场可以与组合物的聚结性能相匹配，使得当施加时，连续流入与组合物的结的加料流体可以直接加入组合物的分散体积 当它穿过交界处时。 还提供了使用组合物和系统的方法。

公开(公告)号：US20160115787A1

公开(公告)日：2016-04-28

申请号：US14922182

申请日：2015-10-25

申请人： Schlumberger Technology Corporation

发明人： Farshid Mostowfi ,  Ronald E. G. van Hal ,  Shahnawaz Hossain Molla ,  Jane T. Lam ,  Amy Du ,  Neil William Bostrom ,  Michael Mallari Toribio

IPC分类号： E21B49/08 ,  B01L3/00

CPC分类号： E21B49/081 ,  B01F13/0072 ,  B01L3/502784 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0487 ,  E21B2049/085

摘要： A technique facilitates detection and analysis of constituents, e.g. chemicals, which may be found in formation fluids and/or other types of fluids. The technique comprises intermittently introducing a first fluid and a second fluid into a channel in a manner which forms slugs of the first fluid separated by the second fluid. The intermittent fluids are flowed through the channel to create a mixing action which mixes the fluid in the slugs. The mixing increases the exchange, e.g. transfer, of the chemical constituent between the second fluid and the first fluid. The exchange aids in sensing an amount of the chemical or chemicals for analysis. In many applications, the intermittent introduction, mixing, and measuring can be performed in a subterranean environment.

摘要翻译： 一种技术有助于组分的检测和分析，例如。 可能在地层流体和/或其他类型的流体中发现的化学物质。 该技术包括以形成由第二流体分离的第一流体的块的方式间歇地将第一流体和第二流体引入通道。 间歇流体流过通道以产生混合作用，其混合s lug中的流体。 混合增加交换，例如 在第二流体和第一流体之间转移化学成分。 该交换有助于感测一定量的化学品或化学品进行分析。 在许多应用中，间歇性引入，混合和测量可以在地下环境中进行。

公开(公告)号：US08257666B2

公开(公告)日：2012-09-04

申请号：US13368703

申请日：2012-02-08

申请人： Stephen R. Quake ,  Hou-Pu Chou

发明人： Stephen R. Quake ,  Hou-Pu Chou

IPC分类号： C12M1/02 ,  C12M1/40

CPC分类号： B01L3/02 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0072 ,  B01F13/0093 ,  B01F15/0201 ,  B01F15/024 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0694 ,  C12M1/02 ,  C12Q1/6816 ,  G01N33/53 ,  Y10T137/0318 ,  Y10T137/0329 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T436/11 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/2575 ,  C12Q2565/629

摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e. Polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene.

摘要翻译： 本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其它分子的微制造装置。 本发明的装置和方法可用于通过检测分子（例如数量的分子），例如多核苷酸（例如DNA）或蛋白质（例如抗体）来同时诊断多种疾病。 与杂交多核苷酸或抗原/抗体复合物相关的报道。 在根据本发明的微制造装置中，分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在的检测与来自与结合分子相关的光学可检测（例如荧光）报告物的杂交信号相关。 这些杂交信号可以通过任何合适的方式，例如光学检测，并且可以例如存储在计算机中作为特定基因的存在的表示。

公开(公告)号：US20100120018A1

公开(公告)日：2010-05-13

申请号：US12536129

申请日：2009-08-05

申请人： Stephen R. Quake ,  Hou-Pu Chou

发明人： Stephen R. Quake ,  Hou-Pu Chou

IPC分类号： C12Q1/70 ,  G01N33/536 ,  C12Q1/68 ,  G01N33/53

CPC分类号： B01L3/02 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0072 ,  B01F13/0093 ,  B01F15/0201 ,  B01F15/024 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0694 ,  C12M1/02 ,  C12Q1/6816 ,  G01N33/53 ,  Y10T137/0318 ,  Y10T137/0329 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T436/11 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/2575 ,  C12Q2565/629

摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

摘要翻译： 本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其它分子的微制造装置。 本发明的装置和方法可用于通过检测分子（例如数量的分子），例如多核苷酸（例如DNA）或蛋白质（例如抗体）来同时诊断多种疾病。 与杂交多核苷酸或抗原/抗体复合物相关的报道。 在根据本发明的微制造装置中，分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在的检测与来自与结合分子相关的光学可检测（例如荧光）报告物的杂交信号相关。 这些杂交信号可以通过任何合适的方式，例如光学检测，并且可以例如存储在计算机中作为特定基因的存在的表示。 杂交探针可以固定在形成一部分或暴露于形成闭环的装置的通道或通道的基底上，用于循环样品以主动接触互补探针。 根据本发明的通用芯片可以不仅用DNA而且可以与其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子一起制备。

公开(公告)号：US06911183B1

公开(公告)日：2005-06-28

申请号：US09518895

申请日：2000-03-06

申请人： Kalyan Handique ,  Bishnu Gogoi ,  Mark A. Burns

发明人： Kalyan Handique ,  Bishnu Gogoi ,  Mark A. Burns

IPC分类号： B01F5/04 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  F15B13/00 ,  F15B13/08 ,  F15C5/00 ,  F16K99/00 ,  G01N1/10 ,  G01N1/18

CPC分类号： B01F13/0072 ,  B01F5/0085 ,  B01F5/0403 ,  B01F13/0071 ,  B01F13/0084 ,  B01F13/0093 ,  B01F15/0232 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  B82Y30/00 ,  B82Y40/00 ,  F15B13/0807 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0061 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

摘要： The movement and mixing of microdroplets through microchannels is described employing microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

摘要翻译： 使用包括微滴输送通道，反应区域，电泳模块和辐射检测器的微量装置描述了通过微通道的微滴的运动和混合。 离散的液滴通过蚀刻通道进行差分加热和推进。 电子元件制造在相同的基板材料上，允许将传感器和控制电路并入同一设备。

公开(公告)号：US06767706B2

公开(公告)日：2004-07-27

申请号：US09875438

申请日：2001-06-05

申请人： Stephen R. Quake ,  Hou-Pu Chou

发明人： Stephen R. Quake ,  Hou-Pu Chou

IPC分类号： C12Q168

CPC分类号： B01L3/02 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0072 ,  B01F13/0093 ,  B01F15/0201 ,  B01F15/024 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0694 ,  C12M1/02 ,  C12Q1/6816 ,  G01N33/53 ,  Y10T137/0318 ,  Y10T137/0329 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T436/11 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/2575 ,  C12Q2565/629

摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

公开(公告)号：US20030145894A1

公开(公告)日：2003-08-07

申请号：US10220785

申请日：2003-01-21

发明人： John Robert Burns

IPC分类号： F15C001/06

CPC分类号： B01F13/0072 ,  B01F5/0256 ,  B01F2013/1091 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00813 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/565 ,  G01N35/08 ,  Y10T137/0396 ,  Y10T137/2082 ,  Y10T137/2224

摘要： A capillary reactor distribution device comprising first and second capillary pathways (2, 3) which meet at a junction (5) and a third capillary pathway (4) which leads away from the junction (5), the capillary pathways (2, 3, 4) being dimensioned such that, when first and second immiscible fluids (14, 15) are fed along respectively the first and second capillary pathways (2, 3) under predetermined laminar flow conditions, the first and second fluids (14, 15) chop each other into discrete slugs (16, 17) which pass along the third capillary pathway (4). Molecular mixing between the fluids (14, 15) takes place by way of axial diffusion between adjacent slugs (16, 17) and by way of internal circulation within each slug (16, 17) as the slugs (16, 17) progress along the third capillary pathway (4).

摘要翻译： 一种毛细管反应器分配装置，包括在结（5）处相交的第一和第二毛细通道（2,3）和离开接合点（5）引导的第三毛细通道（4），毛细管路径（2,3， 4）的尺寸使得当在预定的层流条件下第一和第二不混溶流体（14,15）分别沿着第一和第二毛细通路（2,3）进料时，第一和第二流体（14,15） 彼此分离成沿着第三毛细通路（4）通过的离散块塞（16,17）。 流体（14,15）之间的分子混合通过在相邻的块塞（16,17）之间的轴向扩散并且随着絮块（16,17）沿着 第三毛细血管通路（4）。

公开(公告)号：US10018040B2

公开(公告)日：2018-07-10

申请号：US14922182

申请日：2015-10-25

申请人： Schlumberger Technology Corporation

发明人： Farshid Mostowfi ,  Ronald E. G. van Hal ,  Shahnawaz Hossain Molla ,  Jane T. Lam ,  Amy Du ,  Neil William Bostrom ,  Michael Mallari Toribio

IPC分类号： E21B49/08 ,  B01L3/00 ,  B01F13/00

CPC分类号： E21B49/081 ,  B01F13/0072 ,  B01L3/502784 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0487 ,  E21B2049/085

摘要： A technique facilitates detection and analysis of constituents, e.g. chemicals, which may be found in formation fluids and/or other types of fluids. The technique comprises intermittently introducing a first fluid and a second fluid into a channel in a manner which forms slugs of the first fluid separated by the second fluid. The intermittent fluids are flowed through the channel to create a mixing action which mixes the fluid in the slugs. The mixing increases the exchange, e.g. transfer, of the chemical constituent between the second fluid and the first fluid. The exchange aids in sensing an amount of the chemical or chemicals for analysis. In many applications, the intermittent introduction, mixing, and measuring can be performed in a subterranean environment.

公开(公告)号：US20160024451A1

公开(公告)日：2016-01-28

申请号：US14775285

申请日：2014-02-24

申请人： UNIVERSITY OF TSUKUBA

发明人： Junji FUKUDA ,  Hiroaki SUZUKI ,  Anna YAMAGISHI ,  Junko ENOMOTO ,  Masatoshi YOKOKAWA

IPC分类号： C12M3/06

CPC分类号： B01L3/502784 ,  B01F3/0803 ,  B01F5/0085 ,  B01F13/0061 ,  B01F13/0069 ,  B01F13/0071 ,  B01F13/0072 ,  B01L3/0293 ,  B01L3/5027 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2300/088 ,  C12M23/16 ,  G01N1/38

摘要： A microchannel device includes: an upstream channel portion configured to allow an upstream liquid plug and a gas to flow therethrough; a downstream channel portion configured to allow a downstream liquid plug and a gas to flow therethrough; a liquid holding portion provided between a downstream end portion of the upstream channel portion and an upstream end portion of the downstream channel portion, the liquid holding portion being configured to hold a main liquid plug therein; and a gas bypass channel portion provided so as to bypass the liquid holding portion from the downstream end portion of the upstream channel portion to the upstream end portion of the downstream channel portion, the gas bypass channel portion being configured to allow the gas to flow therethrough in a state in which the liquid holding portion holds the main liquid plug.

摘要翻译： 微通道装置包括：上游通道部分，其构造成允许上游液塞和气体流过其中; 被配置为允许下游液体塞和气体流过其中的下游通道部分; 液体保持部分，设置在上游通道部分的下游端部分与下游通道部分的上游端部分之间，液体保持部分用于将主液体塞塞在其中; 以及气体旁通流路部，其设置成从所述上游流路部的下游端部向所述​​下游流路部的上游侧端部旁通所述液体保持部，所述气体旁通流路部构成为能够使气体流过 在液体保持部保持主液塞的状态下。