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公开(公告)号：US20190060905A1
公开(公告)日：2019-02-28
申请号：US15977824
申请日：2018-05-11
申请人： 10X Genomics, Inc.
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US20220080424A1
公开(公告)日：2022-03-17
申请号：US17357617
申请日：2021-06-24
申请人： 10X Genomics, Inc.
发明人： Ivan AKHREMICHEV , Rajiv BHARADWAJ , Lynna CHEN , Francis CUI , Rachel GERVER , Mohammad Rahimi LENJI , Bill Kengli LIN , Anthony MAKAREWICZ , Martin SAUZADE , Astha TANNA , Fernandino VALDECANAS , Tobias Daniel WHEELER , Yiran ZHANG , Alireza SALMANZADEH
摘要： Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.
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公开(公告)号：US20200290048A1
公开(公告)日：2020-09-17
申请号：US16797727
申请日：2020-02-21
申请人： 10X Genomics, Inc.
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US20230356231A1
公开(公告)日：2023-11-09
申请号：US18223903
申请日：2023-07-19
申请人： 10x Genomics, Inc.
发明人： Michael John TIERNEY , Bill Kengli LIN , Rajiv VERMA , Lynna CHEN , Kevin GILMARTIN , Rajiv BHARADWAJ
CPC分类号： B01L7/52 , B01L3/502707
摘要： The invention provides methods for rapid setting, adjusting, and controlling the temperature of a microfluidic device.
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公开(公告)号：US20200114358A1
公开(公告)日：2020-04-16
申请号：US16706313
申请日：2019-12-06
申请人： 10X Genomics, Inc.
IPC分类号： B01L3/00 , B01F5/04 , B01F11/02 , B01F13/00 , B01F3/08 , G01N33/543 , G01N33/58 , C12Q1/6806 , B01L3/02
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US20190060906A1
公开(公告)日：2019-02-28
申请号：US15977861
申请日：2018-05-11
申请人： 10X Genomics, Inc.
CPC分类号： B01L3/0241 , B01F3/0807 , B01F3/0811 , B01F5/0473 , B01F5/0478 , B01F5/048 , B01F11/0241 , B01F13/0062 , B01F13/0071 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0851 , B01L2300/161 , B01L2400/086 , C12N2533/50 , C12Q1/6806 , C12Q1/686 , C12Q2563/185 , C40B40/06 , G01N33/54313 , G01N33/58 , C12Q2563/149 , C12Q2563/159 , C12Q2565/629
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US20240253044A1
公开(公告)日：2024-08-01
申请号：US18376276
申请日：2023-10-03
申请人： 10x Genomics, Inc.
IPC分类号： B01L3/00 , B01F23/41 , B01F25/314 , B01F31/80 , B01F33/3011 , B01F33/302 , B01L3/02 , C12Q1/6806 , C12Q1/686 , C40B40/06 , G01N33/543 , G01N33/58
CPC分类号： B01L3/502784 , B01F23/41 , B01F23/4105 , B01F25/3141 , B01F25/31422 , B01F25/31423 , B01F31/84 , B01F33/3011 , B01F33/3021 , B01L3/0241 , B01L3/50273 , B01L3/502761 , C12Q1/6806 , G01N33/54313 , G01N33/58 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0851 , B01L2300/161 , B01L2400/086 , C12N2533/50 , C12Q1/686 , C12Q2563/185 , C40B40/06
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US20230173491A1
公开(公告)日：2023-06-08
申请号：US18161643
申请日：2023-01-30
申请人： 10x Genomics, Inc.
IPC分类号： B01L3/00 , G01N33/543 , B01F23/41 , B01F25/314 , B01F31/80 , B01F33/3011 , B01F33/302 , B01L3/02 , C12Q1/6806 , G01N33/58
CPC分类号： B01L3/502784 , B01L3/502761 , G01N33/54313 , B01F23/41 , B01F23/4105 , B01F25/3141 , B01F25/31422 , B01F25/31423 , B01F31/84 , B01F33/3011 , B01F33/3021 , B01L3/0241 , B01L3/50273 , C12Q1/6806 , G01N33/58 , B01L2300/0851 , B01L2300/161 , B01L2200/0652 , B01L2400/086 , B01L2200/0673 , B01L2200/0647 , C12Q1/686
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US20220280933A1
公开(公告)日：2022-09-08
申请号：US17751267
申请日：2022-05-23
申请人： 10x Genomics, Inc.
发明人： Rajiv BHARADWAJ , Hanyoup KIM , Bill Kengli LIN , Marissa PENNELL , Alireza SALMANZADEH , Martin SAUZADE , Tobias Daniel WHEELER
摘要： Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.
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公开(公告)号：US20200206742A1
公开(公告)日：2020-07-02
申请号：US16812866
申请日：2020-03-09
申请人： 10X Genomics, Inc.
IPC分类号： B01L3/00 , B01F5/04 , G01N33/543 , B01F3/08 , B01F13/00 , B01F11/02 , B01L3/02 , C12Q1/6806 , G01N33/58
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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