公开(公告)号：US20230278038A1

公开(公告)日：2023-09-07

申请号：US18177562

申请日：2023-03-02

申请人： 10x Genomics, Inc.

发明人： Mohammad RAHIMI LENJI ,  Yiran ZHANG ,  Francis MARCOGLIESE ,  Tobias Daniel WHEELER ,  Rajiv BHARADWAJ

IPC分类号： B01L3/00 ,  B01F23/41 ,  B01F33/3011

CPC分类号： B01L3/502792 ,  B01F23/4144 ,  B01F33/3011 ,  B01F23/4105 ,  B01L2300/0867 ,  B01L2200/16 ,  B01L2200/0673 ,  B01L2300/0816

摘要： Devices, systems, and their methods of use, for generating and collecting droplets are provided. Devices use flow focusing arrangements of channels to produce droplets. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20210387195A1

公开(公告)日：2021-12-16

申请号：US17458757

申请日：2021-08-27

申请人： 10x Genomics, Inc.

发明人： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Mohammad Rahimi LENJI ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER ,  Yiran ZHANG

IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  B01F5/04

摘要： Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20230340565A1

公开(公告)日：2023-10-26

申请号：US18097989

申请日：2023-01-17

申请人： 10X GENOMICS, INC.

发明人： Zahra Kamila BELHOCINE ,  Josephine Lee ,  Francesca MESCHI ,  Luz MONTESCLAROS ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Jerald SAPIDA ,  David SUKOVICH ,  Tobias Daniel WHEELER ,  Yifeng YIN

IPC分类号： C12Q1/6806

CPC分类号： C12Q1/6806

摘要： The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20220193667A1

公开(公告)日：2022-06-23

申请号：US17563645

申请日：2021-12-28

申请人： 10x Genomics, Inc.

发明人： Tobias Daniel WHEELER ,  Rajiv BHARADWAJ

IPC分类号： B01L3/00

摘要： A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.

公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

申请人： 10X Genomics, Inc.

发明人： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20220080424A1

公开(公告)日：2022-03-17

申请号：US17357617

申请日：2021-06-24

申请人： 10X Genomics, Inc.

发明人： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Francis CUI ,  Rachel GERVER ,  Mohammad Rahimi LENJI ,  Bill Kengli LIN ,  Anthony MAKAREWICZ ,  Martin SAUZADE ,  Astha TANNA ,  Fernandino VALDECANAS ,  Tobias Daniel WHEELER ,  Yiran ZHANG ,  Alireza SALMANZADEH

IPC分类号： B01L3/00 ,  B01L7/00

摘要： Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

公开(公告)号：US20220280933A1

公开(公告)日：2022-09-08

申请号：US17751267

申请日：2022-05-23

申请人： 10x Genomics, Inc.

发明人： Rajiv BHARADWAJ ,  Hanyoup KIM ,  Bill Kengli LIN ,  Marissa PENNELL ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER

IPC分类号： B01L3/02 ,  B01L3/00

摘要： Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.

公开(公告)号：US20210362157A1

公开(公告)日：2021-11-25

申请号：US17397146

申请日：2021-08-09

申请人： 10x Genomics, Inc.

发明人： Rajiv BHARADWAJ ,  Kevin NESS ,  Tobias Daniel WHEELER

IPC分类号： B01L3/00 ,  G01N1/28 ,  G01N33/487

摘要： Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

公开(公告)号：US20200290049A1

公开(公告)日：2020-09-17

申请号：US16887024

申请日：2020-05-29

申请人： 10X Genomics, Inc.

发明人： Rajiv BHARADWAJ ,  Tobias Daniel WHEELER ,  Kevin NESS ,  Benjamin HINDSON

IPC分类号： B01L3/00 ,  B01F13/00 ,  B01J2/06 ,  C12Q1/6844 ,  B01F3/08

摘要： A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.