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公开(公告)号：US20240272188A1
公开(公告)日：2024-08-15
申请号：US18565187
申请日：2022-05-27
发明人： Eric Chu , Tamas Czimmermann , Benjamin Eldridge , Kenneth Jordan , Ximiao Wen
IPC分类号： G01N35/10
CPC分类号： G01N35/1002 , G01N35/1074 , G01N35/1095
摘要： A system includes a chip-receiving component, a first fluid processing assembly, a second fluid processing assembly, and a fluid communication pathway. The chip-receiving component is to receive a process chip having microfluidic passageways. The first fluid processing assembly is to communicate fluids to microfluidic passageways of a process chip received by the chip-receiving component. The second fluid processing assembly includes a sample support feature to support sample containers. The second fluid processing assembly also includes a plurality of sampling heads to selectively communicate fluids from sample containers supported by the sample support feature. The fluid communication pathway includes a plurality of conduits to provide fluid communication between the first fluid processing assembly and the plurality of sampling heads. The first fluid processing assembly is to further communicate fluids from the fluid communication pathway to microfluidic passageways of a process chip received by the chip-receiving component.
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公开(公告)号：US20240102000A1
公开(公告)日：2024-03-28
申请号：US18276163
申请日：2022-02-08
发明人： Samuel Deutsch , Sangeeta Nath , Ximiao Wen , Wei Cai , Babak Sanii , Benjamin Eldridge
IPC分类号： C12N15/10
CPC分类号： C12N15/1013 , C12N15/1017
摘要： Provided herein is a method of making, comprising transporting reagents to a reactor in a microfluidic path device, wherein the reagents include a synthetic gene of interest, a polymerase, a buffer, a first primer having a first region specific to the synthetic gene of interest, and a second primer, wherein the second primer comprises a poly-T sequence of ≥150 base pairs (bp) or a poly-A sequence of ≥150 bp and a second region specific to the synthetic gene of interest; controlling a temperature of the first reactor to perform a polymerase chain reaction within the microfluidic path device to amplify the synthetic gene of interest using the first primer and the second primer to form a synthetic product including the poly-A sequence of ≥150 bp; and transporting the synthetic product out of the first reactor, wherein the synthetic product comprises a synthetic DNA template for in vitro transcription.
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公开(公告)号：US20240018507A1
公开(公告)日：2024-01-18
申请号：US18216054
申请日：2023-06-29
发明人： Benjamin Eldridge , Ximiao Wen
CPC分类号： C12N15/101 , B01L3/502769 , C12M41/48 , G01N21/01 , B01J19/0046 , B01J19/0093 , B01L2400/0487 , G01N2021/0187 , B01J2219/00313 , B01J2219/00333 , B01J2219/00722 , B01J2219/00759
摘要： Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.
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公开(公告)号：US11571693B2
公开(公告)日：2023-02-07
申请号：US17682038
申请日：2022-02-28
发明人： Benjamin Eldridge , Ximiao Wen
IPC分类号： B01L3/00 , A61K31/7105 , B01F23/40 , B01F33/301 , B01F101/22 , F04B19/00
摘要： The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.
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公开(公告)号：US11325122B2
公开(公告)日：2022-05-10
申请号：US17360263
申请日：2021-06-28
发明人： Benjamin Eldridge , Ximiao Wen
IPC分类号： B01L3/00 , A61K31/7105 , B01F23/40 , B01F33/301 , B01F101/22
摘要： The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.
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公开(公告)号：US20210322938A1
公开(公告)日：2021-10-21
申请号：US17360263
申请日：2021-06-28
发明人： Benjamin Eldridge , Ximiao Wen
IPC分类号： B01F13/00 , A61K31/7105 , B01L3/00 , B01F3/08
摘要： The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.
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公开(公告)号：US11926817B2
公开(公告)日：2024-03-12
申请号：US16989824
申请日：2020-08-10
发明人： Benjamin Eldridge , Ximiao Wen
CPC分类号： C12N15/101 , B01J19/0046 , B01J19/0093 , B01L3/502769 , C12M41/48 , G01N21/01 , B01J2219/00313 , B01J2219/00333 , B01J2219/00722 , B01J2219/00759 , B01L2400/0487 , G01N2021/0187
摘要： Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.
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公开(公告)号：US20230035784A1
公开(公告)日：2023-02-02
申请号：US17964185
申请日：2022-10-12
发明人： Benjamin Eldridge , Ximiao Wen , Eric Chu , Tamas Czimmerman , Kenneth Jordan , Jodi Loo , Babak Sanii
IPC分类号： G01N15/02
摘要： An apparatus includes a process chip and a dynamic light scattering assembly. The process chip includes a fluid chamber including and an optically transmissive material adjacent to the fluid chamber. The process chip is to be removably positioned in relation to the dynamic light scattering assembly. The dynamic light scattering assembly is to direct the light through the optically transmissive material and into the fluid chamber. The dynamic light scattering assembly is further to receive light scattered by particles in fluid in the fluid chamber in response to the first optical fiber emitting light into the fluid chamber and thereby capture light scattering data. A processor determines viscosity of fluid in the fluid chamber based on the captured light scattering data. The processor also determines one or both of size or size distribution of particles in the fluid based the captured light scattering data.
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公开(公告)号：US20220323953A1
公开(公告)日：2022-10-13
申请号：US17727937
申请日：2022-04-25
发明人： Benjamin Eldridge , Ximiao Wen
IPC分类号： B01L3/00 , A61K31/7105 , B01F23/40 , B01F33/301
摘要： The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.
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公开(公告)号：US20210322975A1
公开(公告)日：2021-10-21
申请号：US17360252
申请日：2021-06-28
发明人： Benjamin Eldridge , Ximiao Wen
IPC分类号： B01L3/00
摘要： The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.
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