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    Complex of LNA probe and graphene oxide and nucleic acid detection method using same
    2.
    发明授权
    Complex of LNA probe and graphene oxide and nucleic acid detection method using same 有权

    公开(公告)号：US11814672B2

    公开(公告)日：2023-11-14

    申请号：US17006889

    申请日：2020-08-30

    申请人： Korea Research Institute of Chemical Technology

    发明人： Jieon Lee ,  Woo-keun Kim ,  Seokjoo Yoon ,  Bomi Shin

    IPC分类号： C12Q1/6813

    CPC分类号： C12Q1/6813 ,  C12Q2531/119 ,  C12Q2537/137 ,  C12Q2537/1373 ,  C12Q2600/178

    摘要： The present invention provides a complex of LNA probe and graphene oxide, and a nucleic acid detection method using the same. In the present invention, LNA-containing molecular beacon is conjugated through covalent bonding with graphene oxide, a single strand of the molecular beacon binds to a target nucleic acid to form a complex, and the complex is separated from graphene oxide to induce a fluorescence signal. The molecular beacon and graphene oxide can be covalently bonded to minimize non-specific signals, and a LNA-added molecular beacon is designed in a double strand to detect a very low concentration of target nucleic acid with high sensitivity, as well as a fluorescent signal, and the multiple target nucleic acids can be detected simultaneously through diversification of the fluorescent signal to enable easy and accurate detection of a nucleic acid biomarker whose specific expression level is specifically changed according to diseases and disease progression.

    Probe:antiprobe compositions for high specificity DNA or RNA detection
    3.
    发明授权
    Probe:antiprobe compositions for high specificity DNA or RNA detection 有权

    公开(公告)号：US11667971B2

    公开(公告)日：2023-06-06

    申请号：US14216413

    申请日：2014-03-17

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C07H21/02 ,  C12Q1/6876 ,  C12Q1/6832

    CPC分类号： C12Q1/6876 ,  C12Q1/6832 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2537/163

    摘要： Probe systems and methods are provided for detecting nucleic acid targets using labeled polynucleotide probes and antiprobes that interact together and with complementary targets. These interactions result in signaling changes that indicate target frequency and provide error-checking functions that facilitate single base discrimination. These probe:antiprobe compositions enable real-time PCR detection, end-point detection and microarray detection of microbial species, drug resistant mutants, and cancer related variants. The probe:antiprobe may be an internal probe between two primers or may be a primer-probe. The probe also may be modified by introducing a base mismatch to increase thermodynamic discrimination of a correct versus incorrect target differing by a single base. Probe systems also are provided for use in methods of increasing target amplification and detecting specific single base variants.

    Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
    8.
    发明申请
    Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same 审中-公开
    序列转化和信号放大器DNA级联反应和检测方法使用相同

    公开(公告)号：US20160340710A1

    公开(公告)日：2016-11-24

    申请号：US14998162

    申请日：2015-12-24

    申请人： ABBOTT JAPAN CO., LTD.

    发明人： Makoto Komori ,  Toru Yoshimura

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q2600/178 ,  C12Q2521/301 ,  C12Q2537/1373 ,  C12Q2537/149 ,  C12Q2525/131 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2525/207 ,  C12Q2537/137

    摘要： Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide comprising, in the 5′ to 3′ direction, a first signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of a target nucleic acid; a second oligonucleotide comprising, in the 5′ to 3′ direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide; a third oligonucleotide comprising, in the 5′ to 3′ direction, a third signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the second signal DNA generation sequence of the second oligonucleotide.

    摘要翻译： 公开了用于检测样品中靶核酸的方法。 所述方法包括在聚合酶和内切核酸酶的存在下将样品与第一寡核苷酸接触，所述第一寡核苷酸在5'至3'方向上包含第一信号DNA产生序列，核酸内切酶识别位点和与 3'末端; 第二寡核苷酸，其在5'至3'方向上包含第二信号DNA产生序列，内切核酸酶识别位点和与第一寡核苷酸的第一信号DNA产生序列同源的序列; 第三寡核苷酸，其在5'至3'方向上包含第三信号DNA产生序列，内切核酸酶识别位点和与第二寡核苷酸的第二信号DNA产生序列同源的序列。

    Method for detecting single nucleotide polymorphisms
    9.
    发明授权
    Method for detecting single nucleotide polymorphisms 有权
    检测单核苷酸多态性的方法

    公开(公告)号：US09487839B2

    公开(公告)日：2016-11-08

    申请号：US12569648

    申请日：2009-09-29

    申请人： Eric J. Arts ,  Matthew LaLonde

    发明人： Eric J. Arts ,  Matthew LaLonde

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C12Q1/70

    CPC分类号： C12Q1/703 ,  C12Q1/6827 ,  C12Q2565/519 ,  C12Q2561/125 ,  C12Q2525/161 ,  C12Q2537/1373 ,  C12Q2565/501

    摘要： A method of detecting a single nucleotide polymorphism includes providing a first template polynucleotide, a second polynucleotide comprising a first and second sequence (complementary to a first portion of the first polynucleotide), and a third polynucleotide (complementary to a second portion of the first polynucleotide). The first, second and third polynucleotides are annealed, ligated and optionally, these steps are repeated. A fourth polynucleotide, which is essentially complementary to at least a portion of the first sequence, coupled to a first indicator is then provided. A fifth and/or sixth polynucleotide is provided. The fifth polynucleotide is essentially identical to at least a portion of the second sequence of the second polynucleotide and/or to at least a portion of the third polynucleotide. The sixth polynucleotide is essentially complementary to a portion of the second sequence of the second polynucleotide. The third or fifth polynucleotides may be coupled to a second indicator.

    摘要翻译： 检测单核苷酸多态性的方法包括提供第一模板多核苷酸，包含第一和第二序列（与第一多核苷酸的第一部分互补）的第二多核苷酸和第三多核苷酸（与第一多核苷酸的第二部分互补 ）。 将第一，第二和第三多核苷酸退火，连接，任选地，重复这些步骤。 然后提供与第一指示剂耦合的第四个多核苷酸，其基本上与第一序列的至少一部分互补。 提供了第五和/或第六个多核苷酸。 第五多核苷酸基本上与第二多核苷酸的第二序列的至少一部分和/或第三多核苷酸的至少一部分相同。 第六多核苷酸基本上与第二多核苷酸的第二序列的一部分互补。 第三或第五多核苷酸可以连接到第二指示剂。