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    Dynamic switching biosensor
    5.
    发明申请
    Dynamic switching biosensor 审中-公开

    公开(公告)号：US20170315115A1

    公开(公告)日：2017-11-02

    申请号：US15526019

    申请日：2015-11-12

    申请人： Technische Universiteit Eindhoven

    发明人： Menno Willem José Prins ,  Peter Zijlstra ,  Lucas Brunsveld

    IPC分类号： G01N33/543 ,  C12Q1/68

    CPC分类号： G01N33/54306 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N33/542 ,  G01N33/54313 ,  G01N33/54373 ,  G01N33/6872 ,  C12Q2565/107 ,  C12Q2565/133 ,  C12Q2565/607 ,  C12Q2565/628

    摘要： An analyte [25] in a matrix is sensed using a sensing device having a detection probe [21] conjugated to a mediator-receptor [22] that is not a binder for the analyte. The sensor device is provided with mediators [23] conjugated to analyte-receptors [24], where the mediators are selected to bind to the mediator-receptors, and where the analyte-receptors are selected to bind to the analyte. In some embodiments, the mediators are bound to the detection probe by a tether molecule, or tether molecule fragment, or tether domain. In other embodiments, the mediators are not bound to the detection probe. The presence of the analyte is detected by optically or electrically detecting changes of distance between the mediators and the mediator-receptor, indicative of association and/or dissociation events between mediators and mediator-receptor, the characteristics of which are affected by whether the analyte is bound to the analyte-receptor.

    Primers and methods for nucleic acid amplification
    9.
    发明授权
    Primers and methods for nucleic acid amplification 有权
    用于核酸扩增的引物和方法

    公开(公告)号：US09499860B2

    公开(公告)日：2016-11-22

    申请号：US13579486

    申请日：2011-02-18

    申请人： Besik Kankia

    发明人： Besik Kankia

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q1/6816 ,  C12Q2525/185 ,  C12Q2563/107 ,  C12Q2565/107

    摘要： A primer and method for amplification of a target nucleic acid, the primer adapted to conform into a conformation that dissociates from a complementary strand of DNA duplex. The conformation may have a free energy with more favorable thermodynamics than a corresponding DNA duplex, such as a B-DNA duplex. The dissociation may occur during an extension step of an amplification method, such as polymerase chain reaction. The method can proceed isothermally, and the primers may include intrinsic fluorescence.

    摘要翻译： 用于扩增靶核酸的引物和方法，引物适于符合从DNA双链互补链解离的构象。 该构象可以具有比相应的DNA双链体（例如B-DNA双链体）更有利的热力学自由能。 在扩增方法的延伸步骤（例如聚合酶链式反应）中可能发生解离。 该方法可以等温进行，并且引物可以包括固有荧光。