公开(公告)号：US11649486B2

公开(公告)日：2023-05-16

申请号：US16325247

申请日：2017-08-15

Applicant: Olink Bioscience AB

Inventor： Ulf Landegren ,  Johan Björkesten

IPC: C12Q1/6844 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6827

CPC classification number: C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/131 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/163 ,  C12Q1/6832 ,  C12Q2531/125 ,  C12Q2537/163

Abstract: Nucleic acid probes for detection of a target nucleic acid molecule by an RCA reaction in the presence of the target nucleic acid molecule, comprise a first circular template strand which is capable of acting as a template for RCA, and is protected from RCA in the absence of the target nucleic acid molecule by at least a second protector strand which comprises a region of complementarity to the first template strand and is hybridised thereto to form a double-stranded circular structure containing the first template strand inside the protector strand(s). At least one of the second and/or any further protector strands comprises a target binding site, such that upon binding of the probe to the target nucleic acid molecule the probe is able to undergo a strand displacement reaction which allows RCA of the first template strand. Methods of detecting target analytes use such probes.