公开(公告)号：US20240279713A1

公开(公告)日：2024-08-22

申请号：US18470565

申请日：2023-09-20

申请人： SIGMA-ALDRICH CO. LLC

发明人： Brian Ward ,  Kenneth Heuermann

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/6876

CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/179

摘要： The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.

公开(公告)号：US20210324449A1

公开(公告)日：2021-10-21

申请号：US17354443

申请日：2021-06-22

申请人： Sigma-Aldrich Co., LLC

发明人： Brian Ward ,  Kenneth Heuermann

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/6876

摘要： The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.

公开(公告)号：US20200248236A1

公开(公告)日：2020-08-06

申请号：US16834141

申请日：2020-03-30

申请人： Sigma-Aldrich Co. LLC

发明人： Brian Ward ,  Kenneth Heuermann

IPC分类号： C12Q1/6806 ,  C12Q1/6853 ,  C12N15/10

摘要： The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.

公开(公告)号：US20150072899A1

公开(公告)日：2015-03-12

申请号：US14483875

申请日：2014-09-11

申请人： SIGMA-ALDRICH CO. LLC

发明人： Brian Ward ,  Kenneth Heuermann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q2525/179 ,  C12Q2521/107

摘要： The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.