公开(公告)号：US11861889B2

公开(公告)日：2024-01-02

申请号：US18059846

申请日：2022-11-29

申请人： The University of Tokyo ,  OSAKA UNIVERSITY

发明人： Sadao Ota ,  Issei Sato ,  Katsuhito Fujiu ,  Satoko Yamaguchi ,  Kayo Waki ,  Yoko Itahashi ,  Ryoichi Horisaki

IPC分类号： G01N21/01 ,  G06V10/82 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G06V20/69 ,  G06F18/28 ,  G06F18/21 ,  G06V10/772 ,  G06V10/778 ,  C12M1/34 ,  G01N21/65 ,  G01N15/10

CPC分类号： G06V10/82 ,  C12M1/34 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/147 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/01 ,  G01N21/27 ,  G01N21/64 ,  G01N21/65 ,  G06F18/2178 ,  G06F18/28 ,  G06V10/772 ,  G06V10/7784 ,  G06V20/698 ,  G01N2015/1006 ,  G01N2015/145 ,  G01N2015/1413 ,  G01N2015/1415 ,  G06F2218/12

摘要： An analysis device includes an analysis unit configured to receive scattered light, transmitted light, fluorescence, or electromagnetic waves from an observed object located in a light irradiation region light-irradiated from a light source and analyze the observed object on the basis of a signal extracted on the basis of a time axis of an electrical signal output from a light-receiving unit configured to convert the received light or electromagnetic waves into the electrical signal.

公开(公告)号：US11839876B2

公开(公告)日：2023-12-12

申请号：US16303929

申请日：2017-05-24

申请人： Cellix Limited

发明人： Dmitry Kashanin ,  Igor Shvets ,  Francesco Dicorato

IPC分类号： B01L3/00 ,  G01N15/14

CPC分类号： B01L3/502776 ,  B01L3/502761 ,  G01N15/1404 ,  B01L2200/0636 ,  B01L2200/0689 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0809 ,  G01N2015/149 ,  G01N2015/1413 ,  G01N2015/1415

摘要： An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid comprises a hydrodynamic focussing apparatus for providing a focused stream of particulate containing fluid; and a microfluidic chip. The chip has a plurality of layers and comprises a microfluidic channel that extends through the chip substantially orthogonal to a plane of the layers of the chip, and is in fluid communication with the hydrodynamic focusing apparatus for receipt of a focused steam of particulate containing fluid. The chip also comprises a detection zone comprising at least one pair of electrodes in electrical communication with the microfluidic channel. At least one pair of electrodes comprise an excitation electrode coupled to an AC signal source and a detection electrode configured to detect AC impedance changes in the microfluidic channel between the electrodes resulting from particles passing between the electrodes in the microfluidic channel. Methods of sorting mammalian sperm cells according to sex is also described.

公开(公告)号：US11796449B2

公开(公告)日：2023-10-24

申请号：US16279430

申请日：2019-02-19

申请人： ABS Global, Inc.

发明人： David Appleyard ,  Jeff Betthauser ,  Marjorie Faust ,  John Larsen ,  Guocheng Shao ,  Zheng Xia ,  Yu Zhou

IPC分类号： G01N15/14 ,  G01N33/483 ,  G01N21/01 ,  B01L3/00 ,  C12N13/00 ,  G01N1/44 ,  G01N21/64 ,  G01N15/10

CPC分类号： G01N15/1484 ,  B01L3/502761 ,  C12N13/00 ,  G01N1/44 ,  G01N15/1404 ,  G01N21/01 ,  G01N21/6486 ,  G01N33/4833 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0887 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1413

摘要： A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.

公开(公告)号：US20230237789A1

公开(公告)日：2023-07-27

申请号：US18059846

申请日：2022-11-29

申请人： The University of Tokyo ,  OSAKA UNIVERSITY

发明人： Sadao Ota ,  Issei Sato ,  Katsuhito Fujiu ,  Satoko Yamaguchi ,  Kayo Waki ,  Yoko Itahashi ,  Ryoichi Horisaki

IPC分类号： G06V10/82 ,  G01N15/14 ,  G01N21/01 ,  G01N21/27 ,  G01N21/64 ,  G06V20/69 ,  G06F18/28 ,  G06F18/21 ,  G06V10/772 ,  G06V10/778 ,  C12M1/34 ,  G01N21/65

CPC分类号： G06V10/82 ,  G01N15/14 ,  G01N21/01 ,  G01N21/27 ,  G01N21/64 ,  G01N15/147 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1459 ,  G01N15/1434 ,  G06V20/698 ,  G06F18/28 ,  G06F18/2178 ,  G06V10/772 ,  G06V10/7784 ,  C12M1/34 ,  G01N21/65 ,  G01N2015/1413 ,  G01N2015/145 ,  G01N2015/1006

摘要： An analysis device includes an analysis unit configured to receive scattered light, transmitted light, fluorescence, or electromagnetic waves from an observed object located in a light irradiation region light-irradiated from a light source and analyze the observed object on the basis of a signal extracted on the basis of a time axis of an electrical signal output from a light-receiving unit configured to convert the received light or electromagnetic waves into the electrical signal.

公开(公告)号：US20180065120A1

公开(公告)日：2018-03-08

申请号：US15810232

申请日：2017-11-13

申请人： Owl biomedical, Inc.

发明人： John S. FOSTER ,  Kevin SHIELDS ,  Mehran Hoonejani

IPC分类号： B01L3/00 ,  F16K99/00 ,  G01N15/14 ,  G01N21/64

CPC分类号： B01L3/502761 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/0622 ,  B01L2400/0633 ,  F16K99/0011 ,  F16K99/0013 ,  F16K99/0028 ,  F16K99/0046 ,  F16K2099/0084 ,  G01N15/1404 ,  G01N15/1484 ,  G01N21/6402 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2015/1006 ,  G01N2015/1413 ,  G01N2015/142 ,  G01N2015/149 ,  G01N2021/6439 ,  G01N2201/06113

摘要： A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which has a sample inlet channel, output channels, and a movable member formed on a substrate. The device may be used to separate a target particle from non-target material in a sample stream. In order to improve the sorter speed, accuracy or yield, the particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the sample inlet channel. The particle manipulation device may have two separate sort output channels, wherein the sort channel used depends on the characteristics of the sort pulse delivered to the micromechanical particle manipulation device.

公开(公告)号：US09880158B2

公开(公告)日：2018-01-30

申请号：US13376103

申请日：2010-06-02

申请人： Jacobus Johannes Maria Van Dongen ,  José Alberto Orfao De Matos Correia E Vale ,  Juan Alejandro Flores-Montero ,  Julia Maria Almeida Parra ,  Vincent Henricus Johannes Van Der Velden ,  Sebastian Böttcher ,  Andrew Craig Rawstron ,  Ruth Mary De Tute ,  Ludovic Bernard Simon Lhermitte ,  Vahid Asnafi ,  Ester Mejstríková ,  Tomasz Szczepanski ,  Paulo Jorge Monteiro Da Silva Lucio ,  Marta Marín Ayuso ,  Carlos Eduardo Pedreira

发明人： Jacobus Johannes Maria Van Dongen ,  José Alberto Orfao De Matos Correia E Vale ,  Juan Alejandro Flores-Montero ,  Julia Maria Almeida Parra ,  Vincent Henricus Johannes Van Der Velden ,  Sebastian Böttcher ,  Andrew Craig Rawstron ,  Ruth Mary De Tute ,  Ludovic Bernard Simon Lhermitte ,  Vahid Asnafi ,  Ester Mejstríková ,  Tomasz Szczepanski ,  Paulo Jorge Monteiro Da Silva Lucio ,  Marta Marín Ayuso ,  Carlos Eduardo Pedreira

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N15/14 ,  G01N33/50

CPC分类号： G01N33/5094 ,  G01N15/14 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/532 ,  G01N33/533 ,  G01N2015/1402 ,  G01N2015/1413

摘要： The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

公开(公告)号：US09677989B2

公开(公告)日：2017-06-13

申请号：US15006817

申请日：2016-01-26

申请人： STC.UNM

发明人： Larry A. Sklar ,  Bruce Edwards ,  Frederick Kuckuck

IPC分类号： G01N15/14 ,  G01N35/08 ,  G01N21/64 ,  B01L3/00 ,  G01N35/00 ,  G01N35/10 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N15/1436 ,  B01L3/5085 ,  G01N15/1404 ,  G01N15/1459 ,  G01N21/6428 ,  G01N21/6486 ,  G01N35/0099 ,  G01N35/085 ,  G01N35/1095 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1413 ,  G01N2015/1477 ,  G01N2015/149 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/2575

摘要： The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.

公开(公告)号：US09669403B2

公开(公告)日：2017-06-06

申请号：US14239794

申请日：2013-06-10

申请人： Sony Corporation

发明人： Yosuke Muraki

IPC分类号： G01N15/02 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14

CPC分类号： B01L3/502 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1413 ,  G01N2015/149

摘要： Provided is a microparticle measurement device that can deliver a liquid that is used in the analysis of microparticles in a stable manner. The microparticle measurement device includes a plurality of first tank units to which a liquid is supplied from the outside, and which are respectively arranged in parallel, and a bulb unit that is connected to the plurality of first tank units, and which switches to a state in which it is possible to deliver the liquid to a flow channel through which microparticles flow. According to this microparticle measurement device, in addition to performing liquid delivery from a portion of first tank units, in which the replenishment of liquid has been completed, to a flow channel, it is possible to supply liquid from the outside to first tank units other than the first tank units that are delivering liquid.

公开(公告)号：US20170059459A1

公开(公告)日：2017-03-02

申请号：US15151745

申请日：2016-05-11

申请人： Ativa Medical Corporation

发明人： Daniel R. McPeak ,  Ka Man Lee

IPC分类号： G01N1/38 ,  B01L3/00 ,  G01N1/30

CPC分类号： G01N1/38 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/06 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2400/0457 ,  G01N1/30 ,  G01N1/31 ,  G01N15/1484 ,  G01N33/4915 ,  G01N2001/386 ,  G01N2015/0065 ,  G01N2015/035 ,  G01N2015/1006 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1415 ,  G01N2035/00148 ,  G01N2035/00158

摘要： The present description provides, in some embodiments, an apparatus for mixing a fluid in a circuit having an inlet channel defining a flow path for a fluid including particulate matter, a first reagent channel in fluid communication with the inlet channel and defining a first reagent flow path for a first reagent, the inlet channel and first reagent channel configured to shear the fluid entering the first reagent channel from the inlet channel at a first junction, a shearing channel in fluid communication with the inlet channel and first reagent channel at the first junction, and a diffusion channel in fluid communication with the shearing channel at a second junction, the sheared fluid collectable into the diffusion channel such that the fluid is compressed at least in part by the first reagent to have a thickness close to a diameter of the particulate matter in the fluid.

摘要翻译： 在一些实施例中，本说明书提供了一种用于在具有限定用于包括颗粒物质的流体的流动路径的入口通道的回路中混合流体的装置，与入口通道流体连通并限定第一试剂流的第一试剂通道 第一试剂的通路，入口通道和第一试剂通道，其构造成在第一接合处从入口通道剪切进入第一试剂通道的流体，与入口通道流体连通的剪切通道和第一接合处的第一试剂通道 以及在第二连接处与剪切通道流体连通的扩散通道，所述剪切流体可收集到扩散通道中，使得流体至少部分地被第一试剂压缩以具有接近颗粒直径的厚度 在流体中的物质。

公开(公告)号：US09535000B2

公开(公告)日：2017-01-03

申请号：US14479132

申请日：2014-09-05

申请人： Bio-Rad Laboratories, Inc.

发明人： Kun Guo ,  Amir M. Sadri ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC分类号： G01N33/00 ,  G01N1/18 ,  B01L3/00 ,  F04B19/00 ,  G01N15/14 ,  B01L3/02 ,  C12Q1/24

CPC分类号： G01N15/1459 ,  B01L3/0265 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1404 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

摘要： Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

摘要翻译： 用于微流体操纵，分配和/或分选颗粒例如细胞和/或珠粒的系统，包括装置和方法。