PARTICLE ANALYZERS AND PARTICLE TEST CONTROL METHODS AND DEVICES THEREOF

    公开(公告)号:US20170276642A1

    公开(公告)日:2017-09-28

    申请号:US15603384

    申请日:2017-05-23

    IPC分类号: G01N27/416

    摘要: The present disclosure provides a particle analyzer and a particle test control method and device thereof. A method comprises, after acquiring a diluted sample, preserving a part of the diluted sample, and monitoring whether a pore blocking event occurs during a counting process; when the pore blocking event occurs, suspending the test of the sample, and performing an unblocking operation; and after the unblocking operation is completed, controlling a liquid addition system to again acquire the preserved part of the sample from a reaction cell or a tube of the liquid addition system and inject it into a counting cell, and then re-counting the sample in the counting cell by an impedance method. The method makes full use of the residual diluted sample for a second test to eliminate the impact of pore blocking that occurs in the first measurement of the sample on the test result, and there is no need to be place the sample tube again at test position for re-acquisition and re-dilution, thereby reducing the probability of pore blocking.

    MICROFLUIDIC INTERROGATION DEVICE
    7.
    发明申请
    MICROFLUIDIC INTERROGATION DEVICE 有权
    微流控制器件

    公开(公告)号:US20160282254A1

    公开(公告)日:2016-09-29

    申请号:US15006332

    申请日:2016-01-26

    发明人: Harold E. Ayliffe

    IPC分类号: G01N15/14 G01N15/12

    摘要: A portable, stand-alone microfluidic interrogation device including a microprocessor and a touch-screen display. The touch screen display can receive one or more user input to select a particular particle interrogation procedure, and subsequently show interrogation results. A microfluidic path extending through the interrogation device, includes alignment structure that defines an interrogation zone in which particles carried in a fluid are urged toward single-file travel. Operable alignment structure may define sheath-, or non-sheath fluid flow. Desirably, a portion of the alignment structure is removable from the. device in a tool-free procedure, The device may operate under the Coulter principle, and/or detect Stokes' shift phenomena, and/or other optically-based signal(s).

    摘要翻译: 一种便携式,独立的微流体询问装置,其包括微处理器和触摸屏显示器。 触摸屏显示器可以接收一个或多个用户输入以选择特定的粒子询问过程,并随后显示询问结果。 延伸穿过询问装置的微流体路径包括对准结构,该对准结构限定了询问区域,其中流体中携带的颗粒被推向单文件传送。 可操作的对准结构可以限定鞘或非鞘液流。 期望地,对准结构的一部分可从该可移除的位置移除。 该设备可以在库尔特原理下操作,和/或检测斯托克斯的移位现象和/或其他基于光学的信号。

    METHOD AND DEVICE FOR DETECTING SAMPLE
    8.
    发明申请
    METHOD AND DEVICE FOR DETECTING SAMPLE 有权
    用于检测样品的方法和装置

    公开(公告)号:US20160187335A1

    公开(公告)日:2016-06-30

    申请号:US14959133

    申请日:2015-12-04

    摘要: According to one embodiment, a method for detecting a sample includes preparing a device for detecting a sample, the device including a measurement cassette, a first chamber formed by partitioning the cassette with a partition wall, a through-hole provided in the partition wall, a first electrode provided in the cassette, and a second electrode provided in the cassette, introducing a reagent and a sample containing a measuring object substance into the first chamber, introducing a conductive liquid into the second chamber, supplying current to the through-hole, allowing the measuring object substance whose surface is bound to and is covered by the tag particles via the capture substance in the first chamber to pass through the through-hole, and detecting presence of the measuring object substance.

    摘要翻译: 根据一个实施例,一种用于检测样品的方法包括制备用于检测样品的装置,所述装置包括测量盒,通过用隔板分隔盒形成的第一室,设置在分隔壁中的通孔, 设置在盒中的第一电极和设置在盒中的第二电极,将试剂和含有测量对象物质的样品引入到第一室中,将导电液体引入第二室,向通孔提供电流, 允许表面被第一室中的捕获物质结合并被标签颗粒覆盖的测量对象物质通过通孔,并检测测量对象物质的存在。

    System and apparatus for nanopore sequencing
    9.
    发明授权
    System and apparatus for nanopore sequencing 有权
    用于纳米孔测序的系统和仪器

    公开(公告)号:US09322820B2

    公开(公告)日:2016-04-26

    申请号:US13828230

    申请日:2013-03-14

    摘要: A piezoelectric substrate having a nanopore opening that separates two reservoirs of conductive fluid may provide for sensitive biological measurements by allowing control of the size of the nanopore according to piezoelectric stimulation of the substrate. Multiple embodiments are provided of monolithic piezoelectric substrates and nanopores for this purpose as well as a control system for controlling the nanopore dimensions electrically using AC or DC waveforms.

    摘要翻译: 具有分离两个导电流体储存器的纳米孔开口的压电基片可以通过根据基底的压电刺激来控制纳米孔的尺寸来提供灵敏的生物学测量。 提供了用于此目的的单片压电基片和纳米孔的多个实施例,以及用AC或DC波形电控制纳米孔尺寸的控制系统。

    Sample detection apparatus and detection method
    10.
    发明授权
    Sample detection apparatus and detection method 有权
    样品检测装置及检测方法

    公开(公告)号:US09316576B2

    公开(公告)日:2016-04-19

    申请号:US14011666

    申请日:2013-08-27

    摘要: According to one embodiment, a sample detection apparatus including an insulating partition to divide a first and a second region, a pore formed in the partition, a first electrode arranged in the first region, a second electrode arranged in the second region, a power source configured to apply electrical current between the first and second electrode in a state in which a reagent containing a capture substance to be bound to a target and a tag particle bound to the capture substance is introduced into the first region together with a sample, and an electrolyte solution is introduced into the second region, a measurement unit configured to observe a change in a conductive state, and a detection unit to detect presence/absence of the target in the sample based on an observation result.

    摘要翻译: 根据一个实施例,一种样本检测装置,包括分隔第一和第二区域的绝缘隔板,形成在隔板中的孔,布置在第一区域中的第一电极,布置在第二区域中的第二电极,电源 被配置为在所述第一和第二电极之间以与待结合的捕获物质的试剂和与所述捕获物质结合的标签颗粒与试样一起被引入到所述第一区域的状态,在所述第一和第二电极之间施加电流, 电解质溶液被引入到第二区域中,测量单元被配置为观察导电状态的变化,以及检测单元,其基于观察结果来检测样品中的目标的存在/不存在。