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    CELL-BASED ASSAY FOR DETECTION OF ANTIBODIES IN A SAMPLE
    2.
    发明申请
    CELL-BASED ASSAY FOR DETECTION OF ANTIBODIES IN A SAMPLE 有权

    公开(公告)号：US20220091132A1

    公开(公告)日：2022-03-24

    申请号：US17395143

    申请日：2021-08-05

    申请人： Becton, Dickinson and Company

    发明人： Oleg Guryev ,  Majid Mehrpouyan

    IPC分类号： G01N33/68

    摘要： Methods of determining whether a sample includes an antibody that binds to a coronaviral antigen are provided. Aspects of the methods may include combining the sample, a mammalian cell displaying on a surface thereof an antigenic determinant of the coronaviral antigen that is specifically bound by the antibody, and a labeled secondary binding member that binds to the antibody to produce an assay composition; and flow cytometrically assaying the assay composition for the presence of a signal from the labeled secondary binding member to determine whether the sample includes the antibody. Also provided are methods of assessing a subject for an immune response to a coronaviral infection and methods of producing a mammalian cell displaying on a surface thereof an antigenic determinant of a coronaviral antigen. Also provided are systems, expression vectors, mammalian cells, and kits for practicing the subject methods.

    Phase-calibration for imaging flow cytometry
    4.
    发明授权
    Phase-calibration for imaging flow cytometry 有权

    公开(公告)号：US11275026B2

    公开(公告)日：2022-03-15

    申请号：US16861724

    申请日：2020-04-29

    申请人： Becton, Dickinson and Company

    发明人： Matthew Bahr ,  Eric D. Diebold ,  Majid Mehrpouyan ,  Keegan Owsley

    IPC分类号： G01N21/64 ,  G01N15/14

    摘要： Aspects of the present disclosure include methods for phase correcting signals from a light detection system (e.g., in a flow cytometer). Methods according to certain embodiments include detecting light from a sample having particles in a flow stream with a light detection system that includes a brightfield photodetector configured to generate a brightfield data signal and a fluorescence detector configured to generate a fluorescence data signal and calculating a phase correction for the fluorescence detector based on the relative phase between the brightfield data signal and the fluorescence data signal. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate a phase correction for a fluorescence detector based on a brightfield data signal and a fluorescence data signal from the fluorescence detector are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    SERS NANOPARTICLES AND METHODS FOR USING THE SAME
    9.
    发明申请
    SERS NANOPARTICLES AND METHODS FOR USING THE SAME 审中-公开
    SERS纳米颗粒及其使用方法

    公开(公告)号：US20160327551A1

    公开(公告)日：2016-11-10

    申请号：US15108555

    申请日：2015-01-23

    申请人： BECTON, DICKINSON AND COMPANY

    发明人： Majid Mehrpouyan ,  Ming Yan

    IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N21/65 ,  C09K11/06

    CPC分类号： G01N33/54346 ,  B82Y15/00 ,  C09K11/06 ,  C09K2211/1088 ,  G01N21/648 ,  G01N21/658 ,  G01N33/54393 ,  G01N33/587

    摘要： Provided are SERS nanoparticles. In certain aspects, the SERS nanoparticles are fluorescently labeled SERS nanoparticles that include a SERS nanoparticle and a fluorescent label stably associated with a surface of the SERS nanoparticle. Aspects of the present disclosure further include methods of using the SERS nanoparticles, e.g., in a variety of different applications, as well as kits that find use in practicing embodiments of the methods.

    摘要翻译： 提供SERS纳米粒子。 在某些方面，SERS纳米颗粒是荧光标记的SERS纳米颗粒，其包括SERS纳米颗粒和与SERS纳米颗粒表面稳定相关的荧光标记。 本公开的方面进一步包括使用SERS纳米颗粒的方法，例如在各种不同的应用中，以及在实践方法的实施方案中使用的试剂盒。

    Phosphorothioate Oligonucleotide-Labeling of White Blood Cells
    10.
    发明申请
    Phosphorothioate Oligonucleotide-Labeling of White Blood Cells 有权
    硫代磷酸酯寡核苷酸标记白细胞

    公开(公告)号：US20150087005A1

    公开(公告)日：2015-03-26

    申请号：US14364055

    申请日：2012-12-17

    申请人： Becton, Dickinson and Company

    发明人： Majid Mehrpouyan ,  Marybeth Sharkey

    IPC分类号： G01N1/44 ,  G01N33/58

    CPC分类号： G01N1/44 ,  C07K16/2803 ,  G01N33/491 ,  G01N33/56972 ,  G01N33/582 ,  G01N2333/70596

    摘要： The present invention provides labeling and capture reagents that comprise phosphorothioate oligonucleotides ODN). The PS-ODN bind to all white blood cells (leukocytes) in an indiscriminative fashion and enable the labeling, capture, or concentration of leukocytes in a manner that preserves the antigenic integrity of the cells. Methods for the use of phosphorothioate oligonucleotides are provided.

    摘要翻译： 本发明提供包含硫代磷酸酯寡核苷酸ODN的标记和捕获试剂。 PS-ODN以不分皂白的方式结合所有白细胞（白细胞），并能以保持细胞抗原完整性的方式标记，捕获或浓缩白细胞。 提供了使用硫代磷酸酯寡核苷酸的方法。