公开(公告)号：US20240309334A1

公开(公告)日：2024-09-19

申请号：US18121340

申请日：2023-03-14

申请人： ReGen Theranostics, Inc.

发明人： Timothy Nelson

IPC分类号： C12N5/074 ,  A01N1/02 ,  C12N15/86

CPC分类号： C12N5/0696 ,  A01N1/0284 ,  C12N15/86 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/60 ,  C12N2506/1307 ,  C12N2740/15043

摘要： In some embodiments, the present disclosure is directed to methods of reprogramming fibroblasts into induced pluripotent stem cells. In some embodiments, a method comprises obtaining fibroblasts, such as from a skin biopsy. In some embodiments, a method comprises reprogramming fibroblasts using a viral vector encoding reprogramming factors. In some embodiments, a method comprises expanding iPSC and identifying confluent iPSC.

公开(公告)号：US12075773B2

公开(公告)日：2024-09-03

申请号：US18096671

申请日：2023-01-13

申请人： Upkara, Inc.

发明人： Pravansu S. Mohanty ,  Nilay Chakraborty

IPC分类号： A01N1/02 ,  C12M1/00

CPC分类号： A01N1/0284 ,  A01N1/0242 ,  A01N1/0252 ,  A01N1/0263 ,  A01N1/0278 ,  C12M47/14

摘要： Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.

公开(公告)号：US20240215570A1

公开(公告)日：2024-07-04

申请号：US18554688

申请日：2022-04-14

申请人： Abionyx Pharma SA

发明人： Cyrille TUPIN ,  Ronald BARBARAS ,  Loreto GESUALDO ,  Rossana FRANZIN ,  Alessandra STASI

IPC分类号： A01N1/02

CPC分类号： A01N1/0226 ,  A01N1/0247 ,  A01N1/0284

摘要： Lipid binding protein-based complexes for use in an organ preservation solution, organ preservation solutions comprising lipid binding protein-based complexes, kits for making organ preservation solutions, processes for preserving organs using the organ preservation solutions, organs preserved thereby, systems for preserving organs comprising the organ preservation solutions, and methods for transplanting organs obtained by the organ preservation processes.

公开(公告)号：US20240206458A1

公开(公告)日：2024-06-27

申请号：US18576859

申请日：2022-07-12

申请人： Novo Nordisk A/S

发明人： Arvind Pradip ,  Janne Jensen ,  Michael Wagner Christiansen ,  Amna Magzoub

IPC分类号： A01N1/02

CPC分类号： A01N1/0284 ,  A01N1/0252

摘要： The present invention relates to a method for establishing a cell bank, comprising the steps of providing a batch of cell suspension comprising stem cells or stem cell-derived cells, and a cryopreservation medium, maintaining the batch of cell suspension at a temperature below 15° C., and transferring an amount of the cell suspension from the batch of cell suspension into one or more storage containers, wherein establishing the cell bank takes at least (30) minutes.

公开(公告)号：US12016328B2

公开(公告)日：2024-06-25

申请号：US17497330

申请日：2021-10-08

申请人： Genentech, Inc.

发明人： Angela Meier ,  Steven J. Meier ,  Phillip Duffy ,  Marcia Coyne ,  Kara Calhoun

IPC分类号： A01N1/02 ,  C12N5/00 ,  C12N5/071 ,  C12N5/10 ,  C12N15/16

CPC分类号： A01N1/0221 ,  A01N1/0284 ,  C12N5/0018 ,  C12N5/0682 ,  C12N2510/02

摘要： Provided herein are methods of freezing mammalian cells for storage or improving thaw recovery of cell banks comprising freezing mammalian cells in a freezing medium having a pH of 6.7 to 8.5.

公开(公告)号：US20240164371A1

公开(公告)日：2024-05-23

申请号：US18352942

申请日：2023-07-14

申请人： OSSIUM HEALTH, INC.

发明人： Erik J. WOODS ,  Brian H. JOHNSTONE ,  Dongsheng GU ,  Aubrey Marie SHERRY ,  Kelsey Gwen MUSALL

IPC分类号： A01N1/02

CPC分类号： A01N1/0284 ,  A01N1/0221 ,  A01N1/0252

摘要： Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow

公开(公告)号：US11981921B2

公开(公告)日：2024-05-14

申请号：US18337454

申请日：2023-06-20

申请人： Iovance Biotherapeutics, Inc.

发明人： Tim Erickson ,  Andrew Yuhas ,  Rafael Cubas

IPC分类号： C12N5/078 ,  A01N1/02 ,  C12N5/0783

CPC分类号： C12N5/0636 ,  A01N1/0284 ,  C12N2501/2302 ,  C12N2501/2314 ,  C12N2501/2321 ,  C12N2501/515 ,  C12N2501/727

摘要： Provided herein are methods of producing TILs via (i) pre-REP stimulation with a combination of interferon gamma (IFNγ) and an anti-PD-1 antibody, with or without a CD40 agonist, and with or without an anti-CTLA-4 antibody (ii) various concentrations of IL-15 and IL-21, with or without low-concentration IL-2, with or without an AKT inhibitor (AKTi) during REP expansion and/or pre-REP expansion, (iii) low-concentration IL-2 and an AKTi during REP expansion and/or pre-REP expansion, (iv) the combination of (i) and (ii), or (v) the combination of (i) and (iii).

公开(公告)号：US11944718B1

公开(公告)日：2024-04-02

申请号：US18083019

申请日：2022-12-16

申请人： Healthtech Solutions, Inc.

发明人： Bradley Robinson ,  Douglas Ivins Schmid ,  Thayne Sherman Ekness

IPC分类号： A61L27/36 ,  A01N1/02 ,  A61K35/50 ,  A61L27/22 ,  A61L27/60

CPC分类号： A61L27/3604 ,  A01N1/0284 ,  A01N1/0289 ,  A61K35/50 ,  A61L27/227 ,  A61L27/60

摘要： The disclosure herein relates to isolated and purified mammalian fetal allografts and kits and compositions containing these. Also disclosed are methods of preparing isolated and purified mammalian fetal allografts; methods of contacting a wound of a subject with these, and methods of treating a disease or condition with these.

公开(公告)号：US20240067929A1

公开(公告)日：2024-02-29

申请号：US18190879

申请日：2023-03-27

申请人： INSTIL BIO (UK) LIMITED

发明人： Ryan GUEST ,  Joanne MCCAFFREY

IPC分类号： C12N5/0783 ,  A01N1/02 ,  A61K35/17 ,  A61P35/00

CPC分类号： C12N5/0638 ,  A01N1/0221 ,  A01N1/0284 ,  A61K35/17 ,  A61P35/00 ,  C12N5/0636 ,  C12N2501/2302 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2502/30 ,  C12N2509/00

摘要： The present invention provides methods for isolating and cryopreserving tumor infiltrating lymphocytes (TILs) and producing therapeutic populations of TILs, including methods via use of a kit and a semi-automatic device for aseptic disaggregation, enrichment, and cryopreservation of a resected tumor prior to expansion of the TIL population. The present invention also provides methods for expansion, and/or stabilization of TILs, for instance UTILs, compositions involving the same and methods of treatment involving the same.

公开(公告)号：US11903383B2

公开(公告)日：2024-02-20

申请号：US16652831

申请日：2018-10-12

申请人： Asymptote Ltd.

发明人： Peter Kilbride ,  George John Morris

IPC分类号： A01N1/02

CPC分类号： A01N1/0257 ,  A01N1/0268 ,  A01N1/0284 ,  A01N1/0289

摘要： The present invention relates to methods and apparatus for the cryopreservation of biological samples involving a density assisted vitrification wherein a sample of biological material in a cryopreservation agent is cooled from its top surface, causing an ice layer to form thereon. As cooling continues the ice layer grows downwards through the sample to provide a cryoprotectant and biological material rich layer below the ice layer that undergoes vitrification as cooling continues to below the glass transition temperature.