公开(公告)号：US20230152203A1

公开(公告)日：2023-05-18

申请号：US17984116

申请日：2022-11-09

Applicant: Iris International, Inc.

Inventor： Thomas H. Adams ,  Bart J. Wanders ,  John Roche ,  Harvey L. Kasdan

IPC: G01N15/14 ,  G01N33/50 ,  G01N15/06 ,  G01N21/53 ,  G02B7/36 ,  G01N33/80 ,  G01N1/30 ,  G01N33/49

CPC classification number: G01N15/1404 ,  G01N33/5094 ,  G01N15/06 ,  G01N15/1468 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/53 ,  G01N15/1459 ,  G01N15/1429 ,  G01N15/1436 ,  G02B7/36 ,  G01N33/5091 ,  G01N33/80 ,  G01N1/30 ,  G01N33/49 ,  G01N33/4915 ,  G01N2015/1452 ,  G01N2015/0065

Abstract: For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.

公开(公告)号：US20230073269A1

公开(公告)日：2023-03-09

申请号：US17980669

申请日：2022-11-04

Applicant: IRIS INTERNATIONAL, INC.

Inventor： Yong Qin Chen

IPC: G01N15/14 ,  G02B6/293 ,  G02B6/26 ,  F04B11/00 ,  G01N35/10 ,  G02B21/04 ,  G02B21/36 ,  G02B27/00

Abstract: The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

公开(公告)号：US10451612B2

公开(公告)日：2019-10-22

申请号：US15263001

申请日：2016-09-12

Applicant: IRIS International, Inc.

Inventor： Gregory A. Farrell ,  Bart J. Wanders ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/49 ,  G01N33/50 ,  G01N15/14 ,  G01N21/05 ,  G01N15/00 ,  G01N15/10

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.

公开(公告)号：US10209174B2

公开(公告)日：2019-02-19

申请号：US15299677

申请日：2016-10-21

Applicant: IRIS INTERNATIONAL, INC.

Inventor： Yong Qin Chen

IPC: G01N15/14 ,  G02B6/293 ,  G02B6/26 ,  G02B21/04 ,  G02B21/36 ,  G02B27/00 ,  G01N15/10

Abstract: The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

公开(公告)号：US20180143182A1

公开(公告)日：2018-05-24

申请号：US15819298

申请日：2017-11-21

Applicant: Iris International, Inc.

Inventor： Bart J. Wanders ,  Eric Chapoulaud ,  Brett Jordan

IPC: G01N33/50 ,  G01N15/14 ,  G01N21/85 ,  G01N15/00

Abstract: The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. A particle imaging system or analyzer can include a flowcell through which a urine sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis. A contrast pattern for autofocusing is provided on the flowcell. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Cell or particle images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.

公开(公告)号：US20170370820A1

公开(公告)日：2017-12-28

申请号：US15645710

申请日：2017-07-10

Applicant: Iris International, Inc.

Inventor： Bart J. Wanders ,  Gregory A. Farrell ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao ,  Brett Jordan ,  Jack Cremins ,  Carol Quon

IPC: G01N15/14 ,  G01N21/53 ,  G01N33/80 ,  G01N33/49 ,  G01N33/50 ,  G01N1/30 ,  G01N21/05 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Parrticles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

公开(公告)号：US09746412B2

公开(公告)日：2017-08-29

申请号：US14555102

申请日：2014-11-26

Applicant: IRIS INTERNATIONAL, INC.

Inventor： Yong Qin Chen

IPC: G01N15/14 ,  G02B6/26 ,  G02B6/293 ,  G02B21/04 ,  G02B21/36 ,  G02B27/00 ,  G01N15/10

CPC classification number: G01N15/1436 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1452 ,  G02B6/26 ,  G02B6/29365 ,  G02B21/04 ,  G02B21/361 ,  G02B27/0025

Abstract: The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

公开(公告)号：US09322752B2

公开(公告)日：2016-04-26

申请号：US14216533

申请日：2014-03-17

Applicant: IRIS International, Inc.

Inventor： Bart J. Wanders ,  Thomas H. Adams ,  Gregory A. Farrell ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/00 ,  G01N31/00 ,  G01N1/30 ,  G01N33/50 ,  G01N33/80 ,  G01N21/53 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10 ,  G01N21/05

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. In some aspects the system comprises an analyzer which may be a visual analyzer. In one aspect, this disclosure relates to a particle imaging system comprising a flowcell through which a sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis of samples. Other compositions, methods and features of this disclosure are disclosed herein.

Abstract translation: 本公开涉及用于分析含有颗粒的样品的装置，系统，组合物和方法。 在一些方面，该系统包括可以是视觉分析仪的分析器。 一方面，本发明涉及一种粒子成像系统，该粒子成像系统包括流过细胞，通过该流通池使含有颗粒的样品流动;以及高光学分辨率成像装置，其捕获用于样品的图像分析的图像。 本公开的其它组合物，方法和特征在此公开。

公开(公告)号：US20160041166A1

公开(公告)日：2016-02-11

申请号：US14776476

申请日：2014-03-13

Applicant: IRIS INTERNATIONAL, INC.

Inventor： Edward Jablonski ,  Carl Hulle

IPC: G01N33/569 ,  C08F116/06

CPC classification number: G01N33/56938 ,  C08F116/06 ,  C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

Abstract: The present disclosure relates to compositions, methods, and kits for the rapid detection, eparation and/or isolation of microorganisms. Specifically, the disclosure relates to compositions, methods, and kits for using vancomycin—PVA backbone complexes to capture and/or concentrate microorganisms in an aqueous sample, such as gram positive and/or gram negative bacteria in solution.

Abstract translation: 本公开涉及用于快速检测，表达和/或分离微生物的组合物，方法和试剂盒。 具体地说，本公开涉及使用万古霉素-MV骨架复合物捕获和/或浓缩水溶液样品中的微生物的组合物，方法和试剂盒，例如溶液中的革兰氏阳性和/或革兰氏阴性细菌。

公开(公告)号：US20150115174A1

公开(公告)日：2015-04-30

申请号：US14555102

申请日：2014-11-26

Applicant: IRIS INTERNATIONAL, INC.

Inventor： Yong Qin Chen

IPC: G01N15/14

CPC classification number: G01N15/1436 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1452 ,  G02B6/26 ,  G02B6/29365 ,  G02B21/04 ,  G02B21/361 ,  G02B27/0025

Abstract: The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

Abstract translation: 所公开的流式细胞仪包括波分多路复用器（WDM）。 WDM包括提供形成物体的光的扩展光源，从扩展光源捕获光并投影物体的放大图像作为第一光束的准直光学元件，以及配置成将第一聚焦光学元件聚焦 第一光束的尺寸小于扩展光源的对象到第一半导体检测器。 所公开的流式细胞仪还包括复合显微镜物镜，用于将复合显微镜的观察区中的流动通道中的颗粒发射的光引导到扩展光源，流体系统和蠕动泵，其构造成供应液体鞘和液体样品 到流路，以及激光二极管系统来照亮流动通道中的颗粒。