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公开(公告)号：US12066373B2
公开(公告)日：2024-08-20
申请号：US17951719
申请日：2022-09-23
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Austin Payne , Vishal Sharma , Kyle Gleason , Priyadarshini Gogoi , Karthik Ganesan , Brian Boniface , Will Chow
IPC: G01N15/14 , B01L3/00 , C12M1/00 , G01N1/20 , G01N1/28 , G01N1/40 , G01N15/00 , G01N15/1433 , G01N35/00 , G01N35/10 , G06V10/141 , G06V10/145 , G06V10/147 , G06V20/69 , B01L3/02 , G01N15/01 , G01N15/10 , G01N15/149
CPC classification number: G01N15/1484 , B01L3/502715 , B01L3/502761 , C12M47/04 , G01N1/20 , G01N1/28 , G01N1/40 , G01N1/405 , G01N1/4077 , G01N15/1433 , G01N35/00029 , G01N35/1011 , G06V10/141 , G06V10/145 , G06V10/147 , G06V20/69 , B01L3/021 , B01L3/502746 , B01L2200/0652 , B01L2200/0668 , B01L2300/0636 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0819 , B01L2300/0848 , B01L2300/0877 , B01L2300/168 , B01L2400/086 , G01N15/01 , G01N2015/1006 , G01N15/149 , G01N2035/00158 , G01N2035/1039
Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
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公开(公告)号：US20240066519A1
公开(公告)日：2024-02-29
申请号：US18381818
申请日：2023-10-19
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Swati Ranade , Vishal Sharma , Austin Payne , Sam Tuck
CPC classification number: B01L3/502 , C12N13/00 , B01L2200/026 , B01L2300/0893 , B01L2400/043
Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).
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公开(公告)号：US20230037836A1
公开(公告)日：2023-02-09
申请号：US17965495
申请日：2022-10-13
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Vishal Sharma , Priyadarshini Gogoi , William Chow , Austin Payne , Kyle Gleason , Brian Boniface , John Connolly , Sam Tuck
IPC: B01L3/00 , C12M1/00 , G01N33/574 , C12Q1/6816 , G01N33/543 , G01N33/569 , G01N15/14
Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.
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公开(公告)号：US20220155215A1
公开(公告)日：2022-05-19
申请号：US17589507
申请日：2022-01-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Austin Payne , Vishal Sharma , Kyle Gleason , Priyadarshini Gogoi , Karthik Ganesan , Brian Boniface , Will Chow
IPC: G01N15/14 , G01N1/20 , G01N1/40 , G01N1/28 , C12M1/00 , B01L3/00 , G01N35/10 , G01N35/00 , G06V10/141 , G06V10/145 , G06V10/147 , G06V20/69
Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
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公开(公告)号：US11578322B2
公开(公告)日：2023-02-14
申请号：US16867256
申请日：2020-05-05
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Austin Payne , Sida Wang , Patrick Michael Tegels , William T. Koederitz , Daniel Genord , Grey Parker , Brian Boniface , Katlyn Curtin Mehne , Alec William Hitchiner
IPC: C12Q1/68 , C12N15/10 , B01L3/00 , B01L7/00 , G01F23/292 , C12N1/06 , B01L9/02 , G01N35/00 , C12Q1/6844
Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.
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Patent Agency Ranking
公开(公告)号：US20210283608A1
公开(公告)日：2021-09-16
申请号：US17333142
申请日：2021-05-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Vishal Sharma , Priyadarshini Gogoi , William Chow , Austin Payne , Kyle Gleason , Brian Boniface , John Connolly , Sam Tuck
IPC: B01L3/00 , C12M1/00 , G01N33/574 , C12Q1/6816 , G01N33/543 , G01N33/569 , G01N15/14
Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.
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公开(公告)号：US20240255412A1
公开(公告)日：2024-08-01
申请号：US18633224
申请日：2024-04-11
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Austin Payne , Vishal Sharma , Kyle Gleason , Priyadarshini Gogoi , Karthik Ganesan , Brian Boniface , William Chow
IPC: G01N15/14 , B01L3/00 , B01L3/02 , C12M1/00 , G01N1/20 , G01N1/28 , G01N1/40 , G01N15/01 , G01N15/10 , G01N15/1433 , G01N15/149 , G01N35/00 , G01N35/10 , G06V10/141 , G06V10/145 , G06V10/147 , G06V20/69
CPC classification number: G01N15/1484 , B01L3/502715 , B01L3/502761 , C12M47/04 , G01N1/20 , G01N1/28 , G01N1/40 , G01N1/405 , G01N1/4077 , G01N15/1433 , G01N35/00029 , G01N35/1011 , G06V10/141 , G06V10/145 , G06V10/147 , G06V20/69 , B01L3/021 , B01L3/502746 , B01L2200/0652 , B01L2200/0668 , B01L2300/0636 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0819 , B01L2300/0848 , B01L2300/0877 , B01L2300/168 , B01L2400/086 , G01N15/01 , G01N2015/1006 , G01N15/149 , G01N2035/00158 , G01N2035/1039
Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
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公开(公告)号：US20240253038A1
公开(公告)日：2024-08-01
申请号：US18233819
申请日：2023-08-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Vishal Sharma , Priyadarshini Gogoi , William Chow , Austin Payne , Kyle Gleason , Brian Boniface , John Connolly , Sam Tuck
IPC: B01L3/00 , C12M1/00 , C12Q1/6816 , C12Q1/6886 , G01N15/10 , G01N15/14 , G01N15/1434 , G01N15/149 , G01N33/543 , G01N33/569 , G01N33/574
CPC classification number: B01L3/502761 , C12M47/04 , C12Q1/6816 , G01N15/1434 , G01N15/1484 , G01N33/543 , G01N33/569 , G01N33/574 , B01L3/502715 , B01L3/527 , B01L2200/0668 , B01L2300/0636 , B01L2300/0819 , B01L2300/0829 , B01L2300/0851 , B01L2400/0406 , C12Q1/6886 , G01N2015/1006 , G01N15/149
Abstract: A system and method, wherein the method includes: (a) receiving a fluid comprising a process reagent for an assay and nucleic acid material from a sample into a fluid pathway coupled to an array of wells, wherein the array of wells is defined within a substrate and each well in the array of wells extends vertically into a planar surface of the substrate; (b) distributing the fluid along the fluid pathway and into wells of the array of wells; and (c) distributing a liquid immiscible with the fluid comprising the process reagent and nucleic acid material along the fluid pathway upon positive pressure generation by a pump of a flow control system in communication with an inlet to the fluid pathway, thereby displacing said fluid along the fluid pathway and into wells of the array of wells and preventing contents of each well from transferring to adjacent wells.
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公开(公告)号：US11865542B2
公开(公告)日：2024-01-09
申请号：US17965495
申请日：2022-10-13
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Vishal Sharma , Priyadarshini Gogoi , William Chow , Austin Payne , Kyle Gleason , Brian Boniface , John Connolly , Sam Tuck
IPC: B01L3/00 , C12M1/00 , G01N33/574 , C12Q1/6816 , G01N33/543 , G01N33/569 , G01N15/14 , C12Q1/6886 , G01N15/10
CPC classification number: B01L3/502761 , C12M47/04 , C12Q1/6816 , G01N15/1434 , G01N15/1484 , G01N33/543 , G01N33/569 , G01N33/574 , B01L3/502715 , B01L3/527 , B01L2200/0668 , B01L2300/0636 , B01L2300/0819 , B01L2300/0829 , B01L2300/0851 , B01L2400/0406 , C12Q1/6886 , G01N2015/1006 , G01N2015/149
Abstract: A system and method, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity and a fluid delivery module including a fluid pathway through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: generating a set of genetic complexes within individual wells of the array of wells.
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公开(公告)号：US11833507B2
公开(公告)日：2023-12-05
申请号：US17590543
申请日：2022-02-01
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Swati Ranade , Vishal Sharma , Austin Payne , Sam Tuck
CPC classification number: B01L3/502 , C12N13/00 , B01L2200/026 , B01L2300/0893 , B01L2400/043
Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).
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