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112 条记录 (耗时 0.057 秒)

    Methods for HPLC analysis
    1.
    发明授权
    Methods for HPLC analysis 有权

    公开(公告)号：US11912982B2

    公开(公告)日：2024-02-27

    申请号：US16639305

    申请日：2018-08-17

    申请人： ModernaTX, Inc.

    发明人： William Issa ,  Meredith Packer

    IPC分类号： C12N15/10 ,  G01N30/02 ,  G01N30/88

    CPC分类号： C12N15/101 ,  G01N30/02 ,  G01N2030/8827 ,  G01N2496/00

    摘要： Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable high resolution separations of polynucleotides having hydrophobic portions (e.g., polyadenylated nucleic acids, such as mRNA) based upon the hydrophobic character of the molecules (e.g., polyA tail length). In some embodiments, the disclosure describes liquid chromatographic methods for separating a nucleic acid having a hydrophobic portion (e.g., a polyadenylated nucleic acid, such as an mRNA) from a complex mixture by a mobile phase system that comprises an ion pairing agent selected from Tris, inorganic cations (including e.g., Na, Li, K, ammonium, etc.), biological buffers (e.g., MOPS, HEPES, PIPES, etc.), and other charged or hydrophilic moieties, and lacks conventional ion pairing agents (e.g., Triethylammonium acetate, TEAA). Accordingly, in some embodiments methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

    METHODS FOR RNA ANALYSIS
    5.
    发明申请
    METHODS FOR RNA ANALYSIS 审中-公开
    RNA分析方法

    公开(公告)号：US20160304938A1

    公开(公告)日：2016-10-20

    申请号：US15195901

    申请日：2016-06-28

    申请人： CureVac AG

    发明人： Aniela WOCHNER

    IPC分类号： C12Q1/68 ,  B01D15/32 ,  B01D15/16 ,  C07H21/02 ,  G01N30/88

    CPC分类号： C12Q1/6806 ,  B01D15/163 ,  B01D15/325 ,  C07H21/02 ,  G01N30/88 ,  G01N2030/8827 ,  C12Q2521/337 ,  C12Q2565/137 ,  C12Q2565/125

    摘要： The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5′ terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule. In particular, the invention concerns a method for determining the presence of a cap structure in an RNA molecule having a cleavage site for a catalytic nucleic acid molecule, a method for determining the capping degree of a population of RNA molecules having a cleavage site for a catalytic nucleic acid molecule, a method for determining the orientation of the cap structure in a capped RNA molecule having a cleavage site for a catalytic nucleic acid molecule and a method for determining relative amounts of correctly capped RNA molecules and reverse-capped RNA molecules in a population of RNA molecules, wherein the population comprises correctly capped and/or reverse-capped RNA molecules that have a cleavage site for a catalytic nucleic acid molecule. Moreover, the present invention provides uses of a catalytic nucleic acid molecule.

    摘要翻译： 本发明涉及RNA分析领域。 特别地，本发明涉及催化核酸分子用于分析RNA分子的用途。 本发明涉及分析具有催化核酸分子切割位点的RNA分子的5'末端结构的方法。 特别地，本发明涉及一种确定具有催化核酸分子切割位点的RNA分子中帽结构的存在的方法，确定具有切割位点的RNA分子的封端程度的方法 催化核酸分子，用于确定具有催化性核酸分子的切割位点的封端RNA分子中的帽结构的取向的方法，以及用于确定正确加帽的RNA分子和反向封端的RNA分子的相对量的方法 RNA分子的群体，其中群体包含具有催化性核酸分子的切割位点的正确封端和/或反向封端的RNA分子。 此外，本发明提供催化核酸分子的用途。

    METHOD AND APPARATUS FOR ANALYZING A TARGET MATERIAL
    6.
    发明申请
    METHOD AND APPARATUS FOR ANALYZING A TARGET MATERIAL 审中-公开
    用于分析目标材料的方法和装置

    公开(公告)号：US20160187290A1

    公开(公告)日：2016-06-30

    申请号：US15063095

    申请日：2016-03-07

    申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

    发明人： Jean-Pierre Leburton

    IPC分类号： G01N27/414 ,  G01N33/487 ,  G01N27/447

    CPC分类号： G01N33/48721 ,  G01N27/447 ,  G01N27/4473 ,  G01N2030/8827

    摘要： Aspects of the subject disclosure may include, for example, an apparatus having a material having a through-hole, a gate coupled to the material for controlling a charge concentration of the material, a sensor, and a controller coupled to the material, the gate and the sensor. The controller can perform operations including applying a first voltage potential to the material to induce a flow of current in the material, applying a second voltage potential to the gate to adjust the charge concentration of the material, and receiving sensing data from the sensor responsive to a change in electrical properties of the material caused by a target traversing the first through-hole of the material. The through-hole causes a plurality of structural portions of the target to be misaligned with a direction of the flow current in the material. Additional embodiments are disclosed.

    摘要翻译： 本公开的方面可以包括例如具有通孔的材料的装置，与材料相连的栅极，用于控制材料的电荷浓度，传感器和耦合到该材料的控制器，栅极 和传感器。 控制器可以执行操作，包括向材料施加第一电压电位以引起材料中的电流流动，向栅极施加第二电压电位以调整材料的电荷浓度，以及响应于来自传感器的传感器数据接收 由穿过材料的第一通孔的目标引起的材料的电性能的变化。 通孔使靶的多个结构部分与材料中的流动流的方向不对准。 公开了另外的实施例。