公开(公告)号：US10761104B2

公开(公告)日：2020-09-01

申请号：US15593228

申请日：2017-05-11

Applicant: Agilent Technologies, Inc.

Inventor： Reid A. Brennen ,  Bo Curry ,  Joel Myerson ,  Paige Anderson ,  Arthur Schleifer ,  Rolfe Anderson

IPC: G01N35/00 ,  G01N35/10 ,  C12N15/10

Abstract: One or more liquids are transferred from a source array to one or more remotely positioned destination sites such as chambers by utilizing one or more movable transfer elements, such as contact pins or capillaries. The source array may include a predetermined organization of addresses at which materials are positioned. One or more materials may be selected for transfer. Based on the selection, one or more addresses may be accessed by the transfer element(s). The addresses may correspond to spots on a surface of the source array. Each spot may be a feature containing one or more (bio)chemical compounds. At the chamber(s), the material(s) may be processed, such by reaction with one or more reagents. The reaction(s) may entail synthesis of one or more desired products. Alternatively, reaction(s) may be performed at the source array, and the product(s) then transferred to the chamber(s).

公开(公告)号：US20170328928A1

公开(公告)日：2017-11-16

申请号：US15593228

申请日：2017-05-11

Applicant: Agilent Technologies, Inc.

Inventor： Reid A. Brennen ,  Bo Curry ,  Joel Myerson ,  Paige Anderson ,  Arthur Schleifer ,  Rolfe Anderson

IPC: G01N35/10 ,  G01N35/00 ,  C12N15/10

CPC classification number: G01N35/1074 ,  G01N35/1009 ,  G01N35/1065 ,  G01N2035/1037 ,  G01N2035/1039 ,  G01N2035/1044

Abstract: One or more liquids are transferred from a source array to one or more remotely positioned destination sites such as chambers by utilizing one or more movable transfer elements, such as contact pins or capillaries. The source array may include a predetermined organization of addresses at which materials are positioned. One or more materials may be selected for transfer. Based on the selection, one or more addresses may be accessed by the transfer element(s). The addresses may correspond to spots on a surface of the source array. Each spot may be a feature containing one or more (bio)chemical compounds. At the chamber(s), the material(s) may be processed, such by reaction with one or more reagents. The reaction(s) may entail synthesis of one or more desired products. Alternatively, reaction(s) may be performed at the source array, and the product(s) then transferred to the chamber(s).

公开(公告)号：US20130225429A1

公开(公告)日：2013-08-29

申请号：US13758708

申请日：2013-02-04

Applicant: Agilent Technologies, Inc.

Inventor： Bo Curry

IPC: G01N19/04

CPC classification number: G01N19/04 ,  C12Q1/6883 ,  G01N33/557

Abstract: A method for measuring a property of a binding interaction between a capture agent and a binding partner for the capture agent is provided. In certain embodiments, this method comprises: a) contacting a population of particles that are linked to a capture agent with a substrate comprising a binding partner to produce capture agent/binding partner complexes, wherein the population of particles comprises first particles that are bound to a single molecule of the capture agent and second particles that are bound to two molecules of the capture agent; b) applying a force to the bound support, wherein the force is in a direction that separates the particles from the support; and c) separately measuring the forces required to disassociate the first particles and the second particles from their respective complexes.

Abstract translation: 提供了一种用于测量捕获剂与捕获剂的结合配偶体之间的结合相互作用的性质的方法。 在某些实施方案中，该方法包括：a）将与捕获剂连接的颗粒群与包含结合配偶体的底物接触以产生捕获剂/结合配偶体复合物，其中所述颗粒群包含结合至 捕获剂的单分子和与捕获剂的两个分子结合的第二颗粒; b）向结合的支撑件施加力，其中所述力在使所述颗粒与所述支撑体分离的方向上; 和c）分别测量从其各自的复合物分离第一颗粒和第二颗粒所需的力。

公开(公告)号：US20240058813A1

公开(公告)日：2024-02-22

申请号：US17891636

申请日：2022-08-19

Applicant: Agilent Technologies, Inc.

Inventor： Jonathan Irribarren ,  Joel Myerson ,  Bo Curry

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655

Abstract: A fluid delivery device is configured to create a pressure differential across a compartment in which a liquid resides, causing the liquid to flow through the compartment. After a period of time, the fluid delivery device is configured to eliminate the pressure differential and thereby equilibrate the pressure across the compartment, with the use of a pressure equilibration channel that is separate from the compartment. The compartment may contain a packed bed of solid phase particles such as beads. In such case, the pressure differential causes the liquid to flow through the packed bed. The liquid may include chemical reagents or precursors that participate in chemical reactions on or at the solid phase particles. The reactions may relate to chemical synthesis, for example the synthesis of bio-chemicals such as nucleotides.

公开(公告)号：US09683958B2

公开(公告)日：2017-06-20

申请号：US14482803

申请日：2014-09-10

Applicant: Agilent Technologies, Inc.

Inventor： John Mannion ,  Bo Curry ,  Brian Jon Peter

IPC: G01N27/414 ,  G01N27/447

CPC classification number: G01N27/4145 ,  G01N27/4473

Abstract: This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described.

公开(公告)号：US20150361422A1

公开(公告)日：2015-12-17

申请号：US14550713

申请日：2014-11-21

Applicant: Agilent Technologies, Inc.

Inventor： Jeffrey R. Sampson ,  Nicholas M. Sampas ,  Joel Myerson ,  Paige Anderson ,  Bo Curry

IPC: C12N15/10 ,  B01J19/00

Abstract: Provided herein, among other things, is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. Other embodiments are also provided.

Abstract translation: 本文提供了一种方法，其包括：（a）获得多组寡核苷酸的混合物，其中每组中的寡核苷酸各自包含末端分子序列可以组装以产生合成子; 和（b）将寡核苷酸混合物与阵列杂交，从而将不同组的寡核苷酸彼此空间分离。 还提供了其他实施例。

公开(公告)号：US09182334B2

公开(公告)日：2015-11-10

申请号：US13758708

申请日：2013-02-04

Applicant: Agilent Technologies, Inc.

Inventor： Bo Curry

IPC: G01N33/48 ,  G01N33/53 ,  G01N19/04 ,  C12Q1/68 ,  G01N33/557

CPC classification number: G01N19/04 ,  C12Q1/6883 ,  G01N33/557

Abstract: A method for measuring a property of a binding interaction between a capture agent and a binding partner for the capture agent is provided. In certain embodiments, this method comprises: a) contacting a population of particles that are linked to a capture agent with a substrate comprising a binding partner to produce capture agent/binding partner complexes, wherein the population of particles comprises first particles that are bound to a single molecule of the capture agent and second particles that are bound to two molecules of the capture agent; b) applying a force to the bound support, wherein the force is in a direction that separates the particles from the support; and c) separately measuring the forces required to disassociate the first particles and the second particles from their respective complexes.

Abstract translation: 提供了一种用于测量捕获剂与捕获剂的结合配偶体之间的结合相互作用的性质的方法。 在某些实施方案中，该方法包括：a）将与捕获剂连接的颗粒群与包含结合配偶体的底物接触以产生捕获剂/结合配偶体复合物，其中所述颗粒群包含结合至 捕获剂的单分子和与捕获剂的两个分子结合的第二颗粒; b）向结合的支撑件施加力，其中所述力在使所述颗粒与所述支撑体分离的方向上; 和c）分别测量从其各自的复合物分离第一颗粒和第二颗粒所需的力。

公开(公告)号：US20150068901A1

公开(公告)日：2015-03-12

申请号：US14482803

申请日：2014-09-10

Applicant: Agilent Technologies, Inc.

Inventor： John Mannion ,  Bo Curry ,  Brian Jon Peter

IPC: G01N27/447

CPC classification number: G01N27/4145 ,  G01N27/4473

Abstract: This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described.

Abstract translation: 本公开尤其提供了一种纳米流体装置感测装置。 在某些实施例中，该装置包括：a）包括地板和天花板的通道，b）沿通道的纵向轴线布置的通道的地板和/或天花板中的一组电荷传感器; c）在通道的入口端的通道的地板和/或天花板中的捕获区域; 以及d）第一电极和第二电极，其中所述第一和第二电极被定位成沿着所述通道的纵向轴线提供电泳力。 还描述了其它实施例，例如方法。