公开(公告)号：US20240293799A1

公开(公告)日：2024-09-05

申请号：US18644518

申请日：2024-04-24

申请人： Waters Technologies Corporation

发明人： Kevin D. Wyndham ,  Michael F. Morris ,  Darryl W. Brousmiche ,  Jason F. Hill ,  Jacob N. Fairchild

IPC分类号： B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/32 ,  B01D15/40 ,  B01D53/02 ,  B01J20/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07F7/18

CPC分类号： B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  C07F7/1804 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/32 ,  B01J20/3234 ,  B01J2220/54 ,  B01J2220/80

摘要： In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

公开(公告)号：US20240052322A1

公开(公告)日：2024-02-15

申请号：US18257215

申请日：2021-12-13

申请人： Pfizer Inc.

发明人： Mark Thomas Chipley ,  Thomas Wesley Powers

IPC分类号： C12N7/02 ,  B01D15/30 ,  C12N15/86

CPC分类号： C12N7/02 ,  B01D15/305 ,  C12N15/86 ,  C12N2750/14151 ,  C12N2750/14143

摘要： Chromatographic method for separation of AAV capsid proteins using hydrophilic-interaction chromatography (HILIC). The method provides the ability to quantify capsid protein ratio and to separate capsid proteins to the extent that low level post-translational modifications (PTMs) can be detected by mass spectrometry.

公开(公告)号：US20230287038A1

公开(公告)日：2023-09-14

申请号：US18020253

申请日：2021-06-04

申请人： ACCESS TO ADVANCED HEALTH INSTITUTE

发明人： Yizhi QI ,  Christopher Bradford FOX

IPC分类号： C07J63/00 ,  A61K39/39 ,  G01N30/86 ,  G01N30/72 ,  B01D15/30 ,  B01D15/32

CPC分类号： C07J63/008 ,  A61K39/39 ,  G01N30/8631 ,  G01N30/72 ,  B01D15/305 ,  B01D15/325 ,  A61K2039/55577

摘要： Substantially pure saponin extracts and orthogonal chromatographic methods for purification of saponin extracts are disclosed. The purified saponin extracts may include QS-21 and can have a purity of greater than 97%. The orthogonal chromatographic method uses reversed-phase (RP) chromatography followed by hydrophilic interaction liquid chromatography (HILIC) to generate substantially pure saponin extracts.

公开(公告)号：US11747310B2

公开(公告)日：2023-09-05

申请号：US16664403

申请日：2019-10-25

申请人： WATERS TECHNOLOGIES CORPORATION

发明人： Michael F. Morris ,  Matthew A. Lauber ,  Darryl W. Brousmiche

IPC分类号： G01N30/00 ,  G01N30/02 ,  B01D15/30 ,  C07D215/48 ,  G01N30/04 ,  C07H15/26 ,  G01N30/88 ,  G01N33/68 ,  G01N30/62

CPC分类号： G01N30/02 ,  B01D15/305 ,  C07D215/48 ,  C07H15/26 ,  G01N30/04 ,  G01N30/88 ,  G01N33/6842 ,  G01N2030/027 ,  G01N2030/047 ,  G01N2030/626 ,  G01N2030/8836 ,  G01N2400/00 ,  G01N2440/38

摘要： Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.

公开(公告)号：US11433320B2

公开(公告)日：2022-09-06

申请号：US16259705

申请日：2019-01-28

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion

IPC分类号： B01D15/42 ,  B01D15/32 ,  G01N30/34 ,  B01D15/16 ,  B01J20/283 ,  B01J20/287 ,  G01N30/72 ,  G01N30/84 ,  B01D15/30

摘要： The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 0.20% (v/v) difluoroacetic acid and less than about 100 ppb of any individual metal impurity. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US20220034852A1

公开(公告)日：2022-02-03

申请号：US17370589

申请日：2021-07-08

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Stephan M. Koza ,  Pamela C. Iraneta ,  Kevin D. Wyndham

IPC分类号： G01N30/06 ,  G01N30/72 ,  C08F230/08 ,  B01D15/30 ,  C08L33/14 ,  C08F220/54 ,  C08F220/56 ,  B01J20/28 ,  B01J20/32 ,  C08F222/38 ,  B01J20/286

摘要： The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

公开(公告)号：US20210269672A1

公开(公告)日：2021-09-02

申请号：US17258827

申请日：2019-07-08

申请人： Stora Enso OYJ

发明人： Jari Kavakka ,  Staffan Torssell

IPC分类号： C09F3/02 ,  B01D15/18 ,  B01D3/10 ,  B01D3/14 ,  B01D15/30 ,  B01D11/04 ,  G01N30/42

摘要： A method for removing sulfur-containing compounds from crude sulfate turpentine (CST), said method comprising the step of: subjecting CST to continuous liquid-liquid extraction to remove sulfur-containing compounds.

公开(公告)号：US20210230206A1

公开(公告)日：2021-07-29

申请号：US16613546

申请日：2018-05-29

申请人： SPECTRUM THERAPEUTICS GMBH

发明人： Michael ENGLERT ,  Andreas RUTZ

IPC分类号： C07H17/04 ,  C07C37/72 ,  C07D311/78 ,  C07D311/64 ,  C07C7/10 ,  B01D15/18 ,  B01D15/30

摘要： The invention relates to a method for separating natural substance mixtures, in particular those consisting of plant extracts, and for isolating and purifying and obtaining same, by means of sequential centrifugal partition chromatography (sCPC).

公开(公告)号：US20210198116A1

公开(公告)日：2021-07-01

申请号：US16077892

申请日：2018-03-20

申请人： ITM lsotopen Technologien München AG

发明人： Jussi Jernström ,  Konstantin Zhernosekov ,  Mark Harfensteller ,  Nevzat Kelmendi

IPC分类号： C01G17/00 ,  B01J39/05 ,  B01J47/026 ,  B01D15/36 ,  B01D15/18 ,  B01D15/30 ,  B01D15/42

摘要： A method for the manufacture of highly purified 68Ge material for radiopharmaceutical purposes. The invention particularly concerns the production of 68Ge-API (API=Active Pharmaceutical Ingredient) solution complying with the Guidelines for good manufacturing practices (GMP). Starting material for the method of the present invention can be a 68Ge stock solution of commercial or other origin as raw material. Such 68Ge containing raw solutions are purified from potential metal and organic impurities originating from production processes. The radiochemical method disclosed is based on a twofold separation of 68Ge from organic and metallic impurities with two different adsorbent materials. During the first separation phase 68Ge is purified from both organic and metallic impurities by adsorption in germanium tetrachloride form, after which hydrolyzed 68Ge is purified from remaining metallic impurities by cation exchange. The final 68Ge-API-product e.g. fulfills the regulatory requirements for specifications of the GMP production of 68Ge/68Ga generators.

公开(公告)号：US11020686B2

公开(公告)日：2021-06-01

申请号：US16322292

申请日：2017-08-08

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Dingjiang Liu ,  Lin Luo ,  Long Xu

IPC分类号： B01D15/30 ,  C07K16/00 ,  C07K16/10 ,  C07K16/06 ,  G01N30/02 ,  G01N33/68 ,  B01D15/32 ,  A61K39/395

摘要： The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.