公开(公告)号：US11478727B2

公开(公告)日：2022-10-25

申请号：US16848234

申请日：2020-04-14

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion ,  Melvin Blaze Muttikal Thomas ,  Thomas Walter

IPC分类号： B01D15/16 ,  B01D15/30 ,  B01D15/38 ,  B01D3/02 ,  G01N30/72 ,  B01J20/26 ,  B01J20/283 ,  B01J20/22 ,  B01J20/285 ,  B01J20/28 ,  C07C67/54

摘要： The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 2.50% (v/v) difluoroacetic acid and less than about 100 ppb of any individual impurity, especially metal impurities. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US20220268783A1

公开(公告)日：2022-08-25

申请号：US17314452

申请日：2021-05-07

申请人： Waters Technologies Corporation

发明人： Ximo Zhang ,  Stephan M. Koza ,  Ying Qing Yu ,  Weibin Chen

IPC分类号： G01N33/68 ,  G01N30/20 ,  G01N30/22 ,  G01N30/72

摘要： The present disclosure relates to a method of characterizing proteins in a sample. The method includes: removing non-ionic surfactant from the sample via denaturing size-exclusion chromatography to form a denatured sample; eluting the denatured sample via liquid chromatography to collect fractions of the sample, wherein the fractions of the sample include a protein fraction; lyophilizing the protein fraction to increase protein concentration; reconstituting the lyophilized protein fraction with a buffer comprising a surfactant to denature the protein; digesting the denatured protein fraction with an enzyme; and analyzing the digested protein fraction.

公开(公告)号：US20190232197A1

公开(公告)日：2019-08-01

申请号：US16259705

申请日：2019-01-28

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion

IPC分类号： B01D15/42 ,  B01D15/32 ,  G01N30/72 ,  G01N30/84 ,  B01J20/283 ,  B01J20/287

CPC分类号： B01D15/426 ,  B01D15/166 ,  B01D15/305 ,  B01D15/325 ,  B01D15/327 ,  B01J20/283 ,  B01J20/287 ,  G01N30/34 ,  G01N30/7233 ,  G01N30/7266 ,  G01N30/84 ,  G01N2030/8452

摘要： The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 0.20% (v/v) difluoroacetic acid and less than about 100 ppb of any individual metal impurity. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US11433320B2

公开(公告)日：2022-09-06

申请号：US16259705

申请日：2019-01-28

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion

IPC分类号： B01D15/42 ,  B01D15/32 ,  G01N30/34 ,  B01D15/16 ,  B01J20/283 ,  B01J20/287 ,  G01N30/72 ,  G01N30/84 ,  B01D15/30

摘要： The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 0.20% (v/v) difluoroacetic acid and less than about 100 ppb of any individual metal impurity. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US20220009961A1

公开(公告)日：2022-01-13

申请号：US17366443

申请日：2021-07-02

申请人： Waters Technologies Corporation

发明人： Ximo Zhang

IPC分类号： C07K1/34 ,  C07K14/005 ,  B01D21/26 ,  B01D21/00 ,  B01D15/08

摘要： The present disclosure pertains to methods for separating proteins from surfactants that comprise (a) adding an amount of denaturing buffer to a mixture comprising a protein and a surfactant thereby forming a denatured solution wherein the protein is denatured and (b) filtering the denatured solution with a molecular weight cutoff filter, thereby separating the protein from the surfactant and the denaturing buffer. The present disclosure also pertains to kits for forming such methods.

公开(公告)号：US20200261826A1

公开(公告)日：2020-08-20

申请号：US16848234

申请日：2020-04-14

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion ,  Melvin Blaze Muttikal Thomas ,  Thomas Walter

IPC分类号： B01D15/16 ,  B01D15/30 ,  B01D15/38 ,  B01D3/02 ,  G01N30/72 ,  C07C67/54 ,  B01J20/26 ,  B01J20/283 ,  B01J20/22 ,  B01J20/285 ,  B01J20/28

摘要： The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 2.50% (v/v) difluoroacetic acid and less than about 100 ppb of any individual impurity, especially metal impurities. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.