公开(公告)号：US20240293799A1

公开(公告)日：2024-09-05

申请号：US18644518

申请日：2024-04-24

申请人： Waters Technologies Corporation

发明人： Kevin D. Wyndham ,  Michael F. Morris ,  Darryl W. Brousmiche ,  Jason F. Hill ,  Jacob N. Fairchild

IPC分类号： B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/32 ,  B01D15/40 ,  B01D53/02 ,  B01J20/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07F7/18

CPC分类号： B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  C07F7/1804 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/32 ,  B01J20/3234 ,  B01J2220/54 ,  B01J2220/80

摘要： In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

公开(公告)号：US2868011A

公开(公告)日：1959-01-13

申请号：US55357155

申请日：1955-12-16

申请人： GULF RESEARCH DEVELOPMENT CO

发明人： COGGESHALL NORMAN D

IPC分类号： G01N30/46

CPC分类号： G01N30/468 ,  B01D15/1885 ,  B01D15/30 ,  G01N30/461 ,  G01N30/466

公开(公告)号：US11850535B2

公开(公告)日：2023-12-26

申请号：US18067803

申请日：2022-12-19

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Dingjiang Liu ,  Lin Luo ,  Long Xu

IPC分类号： B01D15/30 ,  G01N33/68 ,  B01D15/32 ,  A61K39/395 ,  C07K16/00 ,  C07K16/06 ,  C07K16/10 ,  G01N30/02

CPC分类号： B01D15/30 ,  A61K39/39591 ,  B01D15/327 ,  C07K16/00 ,  C07K16/06 ,  C07K16/065 ,  C07K16/10 ,  G01N30/02 ,  G01N33/6854 ,  G01N2030/027

摘要： The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.

公开(公告)号：US20180036278A1

公开(公告)日：2018-02-08

申请号：US15552331

申请日：2016-02-29

申请人： Bionorica Ethics GmbH

发明人： Andreas Rutz

IPC分类号： A61K31/352 ,  B01D15/30 ,  B01D15/18 ,  G01N30/38 ,  C07D311/80

CPC分类号： A61K31/352 ,  B01D15/1892 ,  B01D15/30 ,  C07D311/80 ,  G01N30/38 ,  G01N2030/381

摘要： The invention relates to cannabinoids and their isolation and purification and to obtaining them by means of centrifugal partition chromatography.

公开(公告)号：US09669402B2

公开(公告)日：2017-06-06

申请号：US13785632

申请日：2013-03-05

申请人： Jiali Liao ,  Russell Frost

发明人： Jiali Liao ,  Russell Frost

IPC分类号： B01D15/26 ,  C07K1/14 ,  B01J20/281 ,  B01J41/20 ,  C07K1/16 ,  B01D15/30 ,  B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/286 ,  B01J20/289 ,  B01J20/32

CPC分类号： B01J41/20 ,  B01D15/265 ,  B01D15/30 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3847 ,  B01J20/22 ,  B01J20/267 ,  B01J20/28016 ,  B01J20/281 ,  B01J20/286 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3208 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3265 ,  B01J20/3293 ,  B01J41/04 ,  B01J41/09 ,  B01J2220/52 ,  C07K1/14 ,  C07K1/16 ,  C07K1/165 ,  C07K16/00 ,  G01N30/482

摘要： Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)—R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.

公开(公告)号：US20140311977A1

公开(公告)日：2014-10-23

申请号：US14358787

申请日：2012-11-20

申请人： Glaxo Group Limited

发明人： Christopher Pink ,  Elin Rundquist

IPC分类号： B01D11/04

CPC分类号： B01D11/0415 ,  B01D15/12 ,  B01D15/1807 ,  B01D15/24 ,  B01D15/30 ,  B01D61/027 ,  B01D2011/002 ,  B01D2311/2626 ,  G01N30/30 ,  G01N2030/027 ,  G01N2030/3084

摘要： A process for separating a compound from solution in organic solvent by a chromatographic process, in particular liquid-liquid chromatography, in which prior to the chromatographic process the composition of the solution is changed by means of a process of organic solvent nanofiltration for a solvent exchange. Additionally or alternatively subsequent to the chromatographic process the output from the chromatographic process is subjected to a process of organic solvent nanofiltration to remove residual target compound and/or impurities in the output for solvent recovery.

摘要翻译： 一种通过色谱法，特别是液相色谱法在有机溶剂中从溶液中分离化合物的方法，其中在色谱法之前，溶液的组成通过有机溶剂纳滤过程进行溶剂交换而改变 。 另外或替代地，在色谱过程之后，色谱过程的输出经过有机溶剂纳滤过程以除去输出中残留的目标化合物和/或杂质用于溶剂回收。

公开(公告)号：US20120138537A1

公开(公告)日：2012-06-07

申请号：US13305045

申请日：2011-11-28

申请人： Brian D. Kelley ,  James E. Booth ,  Paul Brown ,  Jon Coffman ,  Ranganathan Godavarti ,  Tim Iskra ,  Shujun Sun ,  Mary B. Switzer ,  Suresh Vunnum ,  Tianning Yu

发明人： Brian D. Kelley ,  James E. Booth ,  Paul Brown ,  Jon Coffman ,  Ranganathan Godavarti ,  Tim Iskra ,  Shujun Sun ,  Mary B. Switzer ,  Suresh Vunnum ,  Tianning Yu

IPC分类号： B01D15/08

CPC分类号： B01D15/30 ,  B01D15/327 ,  B01D15/361 ,  B01D15/3804 ,  B01D15/3828 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K16/065

摘要： This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.

摘要翻译： 本发明涉及使用弱分配色谱从含有一种或多种杂质的载体流体中纯化产物的方法。 此外，本发明涉及由分配系数至少为0.1的操作条件所限定的弱分配色谱法，该操作条件使培养基与每毫升培养基中的至少1mg产物结合，或者由分配系数限定。

公开(公告)号：USRE24876E

公开(公告)日：1960-09-27

申请号：US86243459

申请日：1959-12-28

IPC分类号： G01N30/46

CPC分类号： G01N30/468 ,  B01D15/1871 ,  B01D15/30 ,  G01N30/461 ,  G01N30/466

公开(公告)号：US12083498B2

公开(公告)日：2024-09-10

申请号：US16926102

申请日：2020-07-10

申请人： Waters Technologies Corporation

发明人： Darryl W. Brousmiche ,  Jacob N. Fairchild ,  Jason F. Hill ,  Giorgis Isaac ,  Michael F. Morris ,  Kevin D. Wyndham

IPC分类号： B01D15/26 ,  B01D15/30 ,  B01D15/32 ,  B01D15/40 ,  B01D53/02 ,  B01J20/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/283 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07F7/18

CPC分类号： B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  C07F7/1804 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/32 ,  B01J20/3234 ,  B01J2220/54 ,  B01J2220/80

摘要： The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.

公开(公告)号：US20230415073A1

公开(公告)日：2023-12-28

申请号：US18458482

申请日：2023-08-30

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Dingjiang LIU ,  Lin LUO ,  Long XU

IPC分类号： B01D15/30 ,  G01N33/68 ,  B01D15/32 ,  A61K39/395 ,  C07K16/00 ,  C07K16/06 ,  C07K16/10 ,  G01N30/02

CPC分类号： B01D15/30 ,  G01N33/6854 ,  B01D15/327 ,  A61K39/39591 ,  C07K16/00 ,  C07K16/065 ,  C07K16/10 ,  C07K16/06 ,  G01N30/02 ,  G01N2030/027

摘要： The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.