公开(公告)号：US20180275088A1

公开(公告)日：2018-09-27

申请号：US15726298

申请日：2017-10-05

Applicant: Abbott Laboratories

Inventor： Jeffrey B. Huff ,  Mark A. Hayden ,  Graham Davis ,  Sergey Gershtein

IPC: G01N27/327 ,  G01N33/487 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/543 ,  C12Q1/00

CPC classification number: G01N27/3272 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/16 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0427 ,  B82Y15/00 ,  C12Q1/004 ,  C12Q1/6869 ,  C40B60/12 ,  G01N21/78 ,  G01N27/3271 ,  G01N33/48707 ,  G01N33/48721 ,  G01N33/54326 ,  G01N33/5438 ,  G01N2021/6482 ,  G01N2021/825 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. Also provided herein are instruments that are programmed to operate a cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region. The instrument may be used to analyse a sample in a cartridge that includes an array of electrodes for actuating a droplet and further includes a nanopore layer for detecting translocation of a tag/second binding member through nanopore. An instrument configured to operate a first cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region and a second cartridge that includes an array of electrodes for actuating a droplet and further includes a nanopore layer for detecting translocation of a tag/second binding member through nanopore is disclosed. An instrument configured to operate a cartridge that includes an array of electrodes for actuating a droplet, an electrochemical species sensing region, and a nanopore layer for detecting translocation of a tag/second binding member through nanopore is disclosed.

公开(公告)号：US10060905B2

公开(公告)日：2018-08-28

申请号：US14359553

申请日：2012-11-20

Applicant: OVIZIO IMAGING SYSTEMS NV/SA

Inventor： Olivier Magniette

IPC: C12Q1/68 ,  G01N33/574 ,  C12P19/34 ,  A61K38/00 ,  C07K16/30 ,  G01N33/487 ,  B01L3/00 ,  G01N21/03 ,  A61B10/02 ,  G01N1/40 ,  G03H1/00 ,  G03H1/04

CPC classification number: G01N33/48778 ,  A61B10/0291 ,  A61B2010/0216 ,  B01L3/502 ,  B01L3/5082 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/023 ,  B01L2300/042 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0832 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2400/086 ,  G01N1/405 ,  G01N21/03 ,  G01N33/57411 ,  G03H1/0443 ,  G03H2001/005

Abstract: The current invention concerns a method for determining the presence of a (pre)cancerous cell in a liquid cell sample, comprising the steps of: —suspending and preserving a cell sample obtained from a subject in a sample vial comprising a liquid medium, said liquid medium comprises means for labeling cells or epitope(s) on or in said cells; —obtaining data from said labelled liquid cell sample; and —determining the presence of said (pre)cancerous cells based on said obtained data; characterized in that said data comprises morphological data and biomarker data. In a further aspect, the invention relates to a liquid medium for fixing, preserving and labeling cells in a cell sample and a sample vial specifically designed to be used in conjunction with the current method.

公开(公告)号：US10054524B2

公开(公告)日：2018-08-21

申请号：US15341131

申请日：2016-11-02

Applicant: RareCyte, Inc.

Inventor： Daniel Campton ,  Joshua Nordberg ,  Steve Quarre ,  David Stewart ,  Ronald Seubert ,  Jonathan Lundt ,  Lance U'Ren ,  Jennifer Chow

IPC: G01N1/40 ,  B01L3/00 ,  B01D21/26 ,  B01L9/00 ,  C12Q1/24 ,  G01N33/574 ,  H01L23/00

CPC classification number: G01N1/4077 ,  B01D21/262 ,  B01L3/50215 ,  B01L3/5635 ,  B01L9/50 ,  B01L2200/026 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2300/0672 ,  B01L2300/0832 ,  B01L2300/0851 ,  B01L2400/0409 ,  B01L2400/0683 ,  C12Q1/24 ,  G01N1/40 ,  G01N33/574 ,  G01N2001/4083 ,  H01L24/03 ,  H01L24/05 ,  H01L24/11 ,  H01L24/13 ,  H01L2224/03912 ,  H01L2224/0401 ,  H01L2224/05541 ,  H01L2224/05647 ,  H01L2224/1134 ,  H01L2224/1146 ,  H01L2224/11462 ,  H01L2224/1147 ,  H01L2224/1162 ,  H01L2224/11849 ,  H01L2224/11901 ,  H01L2224/13022 ,  H01L2224/13025 ,  H01L2224/13082 ,  H01L2224/13111 ,  H01L2224/13155 ,  H01L2924/01028 ,  H01L2924/01029 ,  H01L2924/00014 ,  H01L2924/00012

Abstract: This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. An enrichment agent may be added to a vessel that contains the sample for positive selection, or, in other words, to select or aid in selecting the target material from amongst the remainder of the sample. The enrichment agent may be, for example, immunomagnetic beads, buoyant beads, high-density beads, chemicals to change the density of the target material, or the like.

公开(公告)号：US20180231441A1

公开(公告)日：2018-08-16

申请号：US15948495

申请日：2018-04-09

Applicant: The Regents of the University of California

Inventor： Hsian-Rong Tseng ,  Mitch A. Garcia ,  Min Song ,  Libo Zhao ,  Shuang Hou ,  Tom Lee

IPC: G01N1/28 ,  G01N33/569 ,  G01N33/574 ,  B01L9/00 ,  B01L3/00 ,  G01N1/40

CPC classification number: G01N1/28 ,  B01L3/502715 ,  B01L3/502761 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2300/0609 ,  B01L2300/0816 ,  B01L2300/0883 ,  G01N1/286 ,  G01N1/4077 ,  G01N33/56966 ,  G01N33/574 ,  G01N2001/284 ,  G01N2001/2886

Abstract: A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip.

公开(公告)号：US20180224359A1

公开(公告)日：2018-08-09

申请号：US15943187

申请日：2018-04-02

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Michael D. Henry ,  J. Matthew Barnes

IPC: G01N1/40 ,  B01L3/00 ,  G01N33/50 ,  C12N5/09 ,  G01N33/574 ,  G01N33/487

CPC classification number: G01N1/40 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2400/0475 ,  C12N5/0693 ,  C12N2521/00 ,  G01N33/487 ,  G01N33/5026 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/574

Abstract: Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells.

公开(公告)号：US20180193838A1

公开(公告)日：2018-07-12

申请号：US15861225

申请日：2018-01-03

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song Baca ,  Lewis Spencer Roach

IPC: B01L3/00 ,  C12Q1/44 ,  B01F3/08 ,  B01F5/06 ,  B01F13/00 ,  C12P19/34 ,  B01J19/00 ,  G01N15/14 ,  G01N35/08 ,  B01L7/00 ,  B82Y30/00

CPC classification number: B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

公开(公告)号：US10011865B2

公开(公告)日：2018-07-03

申请号：US15863496

申请日：2018-01-05

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N1/38 ,  B01L3/00 ,  C12Q1/6848 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6827 ,  G01N15/14 ,  B01L7/00

CPC classification number: C12Q1/6848 ,  B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US20180178142A1

公开(公告)日：2018-06-28

申请号：US15901674

申请日：2018-02-21

Applicant: Douglas T. Gjerde

Inventor： Douglas T. Gjerde

IPC: B01D15/22 ,  B01D15/38 ,  B01D15/14 ,  G01N1/34 ,  G01N1/40

CPC classification number: B01D15/22 ,  B01D15/14 ,  B01D15/1807 ,  B01D15/3809 ,  B01L2200/0647 ,  C07K1/22 ,  G01N1/34 ,  G01N1/405 ,  G01N2035/0434

Abstract: This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.

公开(公告)号：US20180161775A1

公开(公告)日：2018-06-14

申请号：US15891579

申请日：2018-02-08

Applicant: The General Hospital Corporation

Inventor： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC: B01L3/00 ,  G01N15/14 ,  A61K35/28 ,  G01N1/40 ,  G01N15/02 ,  G01N15/06 ,  G01N15/00

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：US09989452B2

公开(公告)日：2018-06-05

申请号：US14951322

申请日：2015-11-24

Applicant: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS

Inventor： Ingrid Fritsch ,  Christena Nash ,  Sai Kumar ,  Timothy Muldoon ,  Kartik Balachandran ,  Adair Claycomb ,  Matthew D. Gerner ,  Joshua Hutcheson ,  Foysal Z. Khan ,  Amy Powless ,  Sandra Prieto ,  Preston G. Scrape ,  Melissa C. Weston

IPC: G01N27/26 ,  G01N15/14 ,  B01L3/00 ,  F04B19/00 ,  B01F13/00 ,  G01N15/00 ,  G01N27/447

CPC classification number: G01N15/1475 ,  B01F13/0077 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/043 ,  F04B19/006 ,  G01N15/147 ,  G01N27/447 ,  G01N2015/008 ,  G01N2015/1486

Abstract: A magnetohydrodynamic microfluidic system and a method of pumping a fluid using a magnetohydrodynamic system are disclosed. The method includes applying at least one of an electric current and an electric voltage to a first modified electrode and a second electrode to generate an ionic current between the first modified electrode and the second electrode and to cause a current carrying species to move to or from the modified electrode, applying a magnetic field perpendicular to an ionic current vector, the magnetic field and the ionic current combining to induce flow of the fluid in a direction perpendicular to the magnetic field and the ionic current vector, and maintaining fluid flow by recharging the modified electrode.