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公开(公告)号：US12038438B2
公开(公告)日：2024-07-16
申请号：US17509933
申请日：2021-10-25
发明人： Darren R. Link , Michael L. Samuels
IPC分类号： G01N33/573 , B01F23/41 , B01F25/433 , B01F33/3011 , B01J19/00 , B01L3/00 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6851 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/50 , G01N33/542 , G01N33/58 , B01L7/00 , G01N21/64
CPC分类号： G01N33/573 , B01F23/41 , B01F25/433 , B01F25/4336 , B01F33/3011 , B01J19/0046 , B01L3/502761 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6851 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/5008 , G01N33/542 , G01N33/582 , B01J2219/00286 , B01J2219/00351 , B01J2219/00418 , B01J2219/00479 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00599 , B01J2219/0065 , B01J2219/00657 , B01J2219/00664 , B01J2219/00702 , B01J2219/0072 , B01J2219/00722 , B01J2219/0074 , B01J2219/00743 , B01L7/52 , B01L2200/027 , B01L2300/0636 , B01L2300/0645 , B01L2300/0654 , B01L2300/0681 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , G01N21/6428 , G01N21/6445 , G01N2500/00
摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
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公开(公告)号：US11786872B2
公开(公告)日：2023-10-17
申请号：US16864619
申请日：2020-05-01
IPC分类号： B01F23/41 , B01L3/00 , C12N15/10 , G01N15/14 , B01F25/433 , B01F33/3011 , B01F33/302 , B01F33/3031 , C12P21/00 , B01J19/00 , C12Q1/6874
CPC分类号： B01F23/41 , B01F25/433 , B01F25/4331 , B01F25/4338 , B01F33/3011 , B01F33/3021 , B01F33/3031 , B01J19/0046 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , B01J2219/005 , B01J2219/0052 , B01J2219/00466 , B01J2219/00468 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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13.发明公开公开(公告)号：US20230311087A1
公开(公告)日：2023-10-05
申请号：US18207655
申请日：2023-06-08
申请人： Guangzhou University
发明人： Fan Jiang , Haitao Huang , Meirong Chen , Haoxiang Huang , Yuqin Huang , Ju Yan
IPC分类号： B01J13/04 , B01J13/10 , B01L3/00 , B01F23/41 , B01F33/3011
CPC分类号： B01J13/04 , B01J13/10 , B01L3/502746 , B01F23/4105 , B01F23/4144 , B01F23/4143 , B01F33/3011 , B01L2400/086 , B01L2200/0636 , B01L2300/0861 , B01L2400/0406
摘要： A flow focusing type one-step double emulsion droplet parallel generation device and method. The device comprises a fluid injection module, a liquid droplet generation module, a liquid droplet surface solidification module and a liquid droplet collection module, wherein the fluid injection module is used for conveying fluid of each phase to the liquid droplet generation module; and the liquid droplet generation module comprises a fluid distribution functional area, a liquid droplet preparation functional area and an auxiliary functional arca, wherein the liquid droplet distribution functional area is used for conveying the fluid of each phase into a channel of the fluid of each phase corresponding to the liquid droplet preparation functional area, and the fluid of each phase is gathered at the same point in a flow focusing structure and then is broken, and a fluid of an outer phase covers the fluid of the middle phase.
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公开(公告)号：US20230173491A1
公开(公告)日：2023-06-08
申请号：US18161643
申请日：2023-01-30
申请人： 10x Genomics, Inc.
IPC分类号： B01L3/00 , G01N33/543 , B01F23/41 , B01F25/314 , B01F31/80 , B01F33/3011 , B01F33/302 , B01L3/02 , C12Q1/6806 , G01N33/58
CPC分类号： B01L3/502784 , B01L3/502761 , G01N33/54313 , B01F23/41 , B01F23/4105 , B01F25/3141 , B01F25/31422 , B01F25/31423 , B01F31/84 , B01F33/3011 , B01F33/3021 , B01L3/0241 , B01L3/50273 , C12Q1/6806 , G01N33/58 , B01L2300/0851 , B01L2300/161 , B01L2200/0652 , B01L2400/086 , B01L2200/0673 , B01L2200/0647 , C12Q1/686
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号：US12097495B2
公开(公告)日：2024-09-24
申请号：US17750195
申请日：2022-05-20
发明人： Benjamin J. Hindson , Serge Saxonov , Phillip Belgrader , Kevin D. Ness , Michael Y. Lucero , Billy W. Colston, Jr. , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC分类号： B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
摘要： The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
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公开(公告)号：US12091710B2
公开(公告)日：2024-09-17
申请号：US17174519
申请日：2021-02-12
发明人： Darren Roy Link
IPC分类号： C12Q1/6848 , B01F23/41 , B01F33/3011 , B01J19/00 , B01L3/00 , B03C5/00 , B03C5/02 , C12N15/10 , C12Q1/00 , C12Q1/6806 , C12Q1/6827 , C12Q1/6844 , C12Q1/686 , C12Q1/6874 , G01N1/38 , G01N15/14 , G01N35/00 , G01N35/08 , B01L7/00 , B01L9/00
CPC分类号： C12Q1/6848 , B01F23/41 , B01F33/3011 , B01J19/0093 , B01L3/502715 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L3/565 , B03C5/005 , B03C5/026 , C12N15/1075 , C12Q1/00 , C12Q1/6806 , C12Q1/6827 , C12Q1/6844 , C12Q1/686 , C12Q1/6874 , G01N1/38 , G01N15/147 , G01N35/08 , B01J2219/00783 , B01J2219/00788 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/00853 , B01J2219/0086 , B01J2219/00869 , B01J2219/00889 , B01J2219/00903 , B01J2219/0097 , B01J2219/00988 , B01L3/502746 , B01L7/525 , B01L9/527 , B01L2200/027 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/141 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/165 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/086 , B03C2201/26 , C12Q2525/191 , C12Q2535/101 , C12Q2563/149 , C12Q2563/159 , C12Q2565/628 , C12Q2565/629 , G01N2035/00158 , G01N2035/00237 , G01N2035/00326
摘要： The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.
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公开(公告)号：US12077638B2
公开(公告)日：2024-09-03
申请号：US15335184
申请日：2016-10-26
发明人： John Oakey , Kaspars Krutkramelis , Bingzhao Xia
IPC分类号： A61K36/9066 , A61K9/50 , A61K35/12 , A61L27/16 , A61L27/18 , A61L27/38 , A61L27/52 , A61L27/56 , B01F23/41 , B01F25/433 , B01F33/3011 , B01J2/06 , B01J13/00 , B01J19/00 , C08F2/32 , C08F2/48 , C08J3/075 , C08J9/26 , C12N5/00 , C08F216/12 , C08F222/10
CPC分类号： C08J3/075 , A61K9/5026 , A61K35/12 , A61L27/16 , A61L27/18 , A61L27/38 , A61L27/52 , A61L27/56 , B01F23/41 , B01F25/4331 , B01F33/3011 , B01J2/06 , B01J13/0052 , B01J19/0093 , C08F2/32 , C08F2/48 , C08J9/26 , C12N5/0012 , B01J2219/00792 , B01J2219/00936 , C08F216/125 , C08F222/102 , C08J2201/046 , C08J2205/022 , C08J2207/10 , C08J2335/02 , C08J2345/00
摘要： Provided herein are methods utilizing microfluidics for the oxygen-controlled generation of microparticles and hydrogels having controlled microparticle sizes and size distributions and products from provided methods. The included methods provide the generation of microparticles by polymerizing an aqueous solution dispersed in a non-aqueous continuous phase in an oxygen-controlled environment. The process allows for control of size of the size of the aqueous droplets and, thus, control of the size of the generated microparticles which may be used in biological applications.
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公开(公告)号：US20240150813A1
公开(公告)日：2024-05-09
申请号：US18400468
申请日：2023-12-29
发明人： Benjamin J. Miller , Qun Zhong , Darren Roy Link
IPC分类号： C12Q1/6806 , B01F23/41 , B01F33/3011 , B01F35/221 , B01L3/00 , B05B1/02 , B05B1/08 , B05B1/26 , B05B7/00
CPC分类号： C12Q1/6806 , B01F23/41 , B01F33/3011 , B01F35/2213 , B01L3/502761 , B01L3/502784 , B05B1/02 , B05B1/08 , B05B1/26 , B05B7/0012 , B01L7/525 , B01L2200/0647 , B01L2300/14 , B01L2400/0487 , Y10T436/2575
摘要： The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
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公开(公告)号：US11918961B2
公开(公告)日：2024-03-05
申请号：US16628616
申请日：2018-07-03
发明人： Benoit Scheid , Adrien Dewandre , Youen Vitry
IPC分类号： B01F23/00 , B01F23/232 , B01F23/41 , B01F33/3011 , B01F35/10 , B01F35/21 , B01F35/221 , B01F35/71 , B01J4/00 , B01J13/00 , B33Y10/00 , B33Y80/00
CPC分类号： B01F23/4105 , B01F23/232 , B01F33/3011 , B01F35/10 , B01F35/2111 , B01F35/2213 , B01F35/71745 , B01J4/002 , B01J13/0004 , B33Y10/00 , B33Y80/00 , B01F2215/0431
摘要： A device for generating bubbles or droplets may include a cavity comprising a first pressurized phase, at least one input capillary of a second phase, and an output capillary coaxially aligned with the at least one input capillary. The opening of the tip of the at least one input capillary has an internal diameter of less than half the internal diameter of the output capillary. The cross section of the cavity may be selected so that, in use, the average speed field in the cavity is quasi-static.
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公开(公告)号：US20230219043A1
公开(公告)日：2023-07-13
申请号：US18178965
申请日：2023-03-06
IPC分类号： B01F23/41 , C40B50/08 , G01N33/50 , C40B40/04 , B01J19/00 , B01F25/433 , G01N15/14 , B01L3/00 , B01F33/3011 , B01L7/00 , B01F101/23 , G01N15/10
CPC分类号： B01F23/41 , C40B50/08 , G01N33/5008 , C40B40/04 , B01J19/0046 , B01F25/433 , G01N15/1459 , B01L3/502761 , B01F33/3011 , B01F25/4336 , B01L2400/0487 , B01L2300/0654 , B01J2219/00286 , B01J2219/00479 , B01L7/52 , B01L2300/0636 , B01J2219/0059 , B01J2219/00702 , B01L2200/027 , B01J2219/00599 , B01F2101/23 , B01J2219/00576 , B01J2219/00585 , B01J2219/00592 , B01J2219/0072 , B01J2219/00722 , B01L2300/0864 , B01L2400/0415 , G01N2500/00 , B01J2219/00743 , B01J2219/0074 , B01L2300/0645 , B01J2219/00657 , B01J2219/00418 , B01J2219/00351 , B01L2300/0867 , B01J2219/0065 , B01L2300/0681 , G01N2015/1006 , B01J2219/00664 , Y02A90/10
摘要： The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.
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