公开(公告)号：US12241116B2

公开(公告)日：2025-03-04

申请号：US18536935

申请日：2023-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20240093271A1

公开(公告)日：2024-03-21

申请号：US18514645

申请日：2023-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20230086845A1

公开(公告)日：2023-03-23

申请号：US17992304

申请日：2022-11-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US12031175B2

公开(公告)日：2024-07-09

申请号：US18514645

申请日：2023-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/172 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/6827 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/686 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/6827 ,  C12Q2531/113 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20240132939A1

公开(公告)日：2024-04-25

申请号：US18536935

申请日：2023-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20240002910A1

公开(公告)日：2024-01-04

申请号：US18465623

申请日：2023-09-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11254968B2

公开(公告)日：2022-02-22

申请号：US15688250

申请日：2017-08-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20220213530A1

公开(公告)日：2022-07-07

申请号：US17574780

申请日：2022-01-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20220143617A1

公开(公告)日：2022-05-12

申请号：US17582441

申请日：2022-01-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin Miller ,  Brian Hutchison ,  Andrew Wilson ,  Jonathan William Larson ,  Qun Zhong ,  Yevgeny Yurkovetsky ,  Darren Link ,  Mark Weary

IPC: B01L3/00

Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

公开(公告)号：US10428369B2

公开(公告)日：2019-10-01

申请号：US15176710

申请日：2016-06-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Miller ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6806 ,  B05B1/08 ,  B05B1/26 ,  B05B7/00 ,  B01F13/00 ,  B01F15/00 ,  B01F3/08 ,  B05B1/02 ,  B01L3/00 ,  B01L7/00

Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.