公开(公告)号：US20240352440A1

公开(公告)日：2024-10-24

申请号：US18641530

申请日：2024-04-22

申请人： BONUMOSE, INC.

发明人： Daniel Joseph WICHELECKI ,  Edwin O. ROGERS

IPC分类号： C12N9/90 ,  C12N9/10 ,  C12N9/16 ,  C12N9/92 ,  C12P19/02 ,  C12P19/14 ,  C12P19/18 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/92 ,  C12P19/02 ,  C12P19/14 ,  C12P19/18 ,  C12P19/24 ,  C12Y204/01001 ,  C12Y301/03011 ,  C12Y503/01009 ,  C12Y504/02002

摘要： Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

公开(公告)号：US20240318211A1

公开(公告)日：2024-09-26

申请号：US18587332

申请日：2024-02-26

申请人： 1000128725 Ontario Inc.

发明人： Hamed Hasheminejad ,  Mahdi Shahsavand

IPC分类号： C12P17/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12P7/02

CPC分类号： C12P17/06 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/90 ,  C12P7/02 ,  C12Y205/01001 ,  C12Y205/01039 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y503/03002

摘要： Ways of making and using a recombinant organism configured to produce a cannabinoid are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM). A cannabinoid can be produced by a process that includes growing the recombinant organism configured to produce the cannabinoid in a growth medium and separating the cannabinoid from the recombinant organism and the growth medium. A biosynthetic system for producing a cannabinoid is provided that includes a bioreactor, the recombinant organism configured to produce the cannabinoid, and a growth medium for the recombinant organism.

公开(公告)号：US20240306693A1

公开(公告)日：2024-09-19

申请号：US18263830

申请日：2022-02-02

申请人： BONUMOSE, INC.

发明人： Daniel Joseph WICHELECKI ,  Edwin O. ROGERS

IPC分类号： A23L33/20 ,  A21D2/26 ,  A23L33/17 ,  C12N9/12 ,  C12N9/90 ,  C12N9/92

CPC分类号： A23L33/20 ,  A21D2/26 ,  A23L33/17 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12Y207/01144 ,  C12Y401/02022 ,  C12Y503/01009 ,  C12Y504/02002

摘要： The invention relates to preparation of food ingredients enriched with a low-glycemic sugar replacement through enzymatic conversion. Food ingredients may be enriched with, for example, D-tagatose, D-allulose, D-allose, D-mannose, D-talose, and/or inositol by enzymatically converting saccharides found in flour, meal, ground tuber, ground pulse, ground bark, starch, malted grain or malt extract, maltodextrin, cellulose, cellodextrin, any of their derivatives (e.g., amylose, amylopectin, dextrin, cellobiose, etc.), and/or sucrose into D-tagatose, D-allulose, D-allose, D-mannose, D-talose and/or inositol. The enriched material can be used as a food ingredient instead of the low-glycemic sugar being purified for use as a food ingredient.

公开(公告)号：US20240287551A1

公开(公告)日：2024-08-29

申请号：US18572361

申请日：2022-02-28

申请人： POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

发明人： Gyoo Yeol JUNG ,  Dae Yeol YE ,  Myung Hyun NOH ,  Myeong Won MIN

IPC分类号： C12P7/44 ,  C12N9/10 ,  C12N9/90 ,  C12N15/70

CPC分类号： C12P7/44 ,  C12N9/1029 ,  C12N9/90 ,  C12N15/70 ,  C12Y203/01182 ,  C12Y503/03006

摘要： The present invention relates to a novel metabolic pathway for producing itaconic acid and a recombinant microorganism, for producing itaconic acid, into which the metabolic pathway is introduced. It has been confirmed that the recombinant microorganism, for producing itaconic acid, into which the novel itaconic acid metabolic pathway is introduced, according to the present invention, significantly increases the production and yield of itaconic acid. In addition, as the recombinant microorganism for producing itaconic acid of the present invention uses an intermediate material of glycolysis rather than an intermediate material of the TCA cycle, the recombinant microorganism can further increase the production yield of itaconic acid through further improvement. Accordingly, the novel metabolic pathway for producing itaconic acid and the recombinant microorganism into which the metabolic pathway is introduced can increase the economic feasibility of itaconic acid, and thus can be variously used in industrial fields for synthetic resins, latexes, food additives, and the like in which itaconic acid is utilized.

公开(公告)号：US12071645B2

公开(公告)日：2024-08-27

申请号：US17900590

申请日：2022-08-31

申请人： Givaudan SA

发明人： Eric Eichhorn ,  Boris Schilling ,  Denis Wahler ,  Laurent Fourage ,  Esther Locher

IPC分类号： C12P17/04 ,  C07D307/92 ,  C12N9/90

CPC分类号： C12P17/04 ,  C07D307/92 ,  C12N9/90

摘要： There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (−)-Ambrox and SHC/HAC enzymes in methods to prepare (−)-Ambrox.

公开(公告)号：US12049617B2

公开(公告)日：2024-07-30

申请号：US17050626

申请日：2019-05-21

申请人： DSM IP Assets B.V.

发明人： Christopher Mark Farrell ,  Lisa Ann Laprade ,  Otto Martin Lehmann ,  Joshua Trueheart ,  Bastien Jean Wolfgang Chevreux

IPC分类号： C12N1/16 ,  C12N9/90 ,  C12P33/00

CPC分类号： C12N1/16 ,  C12N9/90 ,  C12P33/00

摘要： The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US12043834B2

公开(公告)日：2024-07-23

申请号：US17525283

申请日：2021-11-12

申请人： Qilu University of Technology

发明人： Hongling Liu ,  Tengfei Wang ,  Ruiming Wang ,  Xinyi Zhang

IPC分类号： C12N15/52 ,  C12N9/10 ,  C12N9/26 ,  C12N9/90 ,  C12N11/18 ,  C12N15/03 ,  C12N15/75

CPC分类号： C12N15/52 ,  C12N9/1051 ,  C12N9/2408 ,  C12N9/90 ,  C12N11/18 ,  C12N15/03 ,  C12N15/75 ,  C12Y302/01141 ,  C12Y504/99015

摘要： The present disclosure relates to a method for constructing a trehalose polyenzyme complex in vitro by mediation of an artificial scaffold protein, which mainly comprises the following steps: constructing a recombinant bacterium WB800n-ScafCCR for self-assembled scaffold protein module; constructing a recombinant bacterium WB800n-P43-phoD -treY-Ccdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-treZ-Ctdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-cgt-Rfdoc for self-assembled catalytic module; secretorily expressing the recombinant bacteria and self-assembling in vitro to obtain a recombinant trehalose multi-enzyme complex. The trehalose multi-enzyme complex constructed by the method of the present disclosure has a higher catalytic efficiency in preparing trehalose than that of mixed free enzymes, and the method can be used for production of high quality trehalose after immobilization with cellulose microspheres.

公开(公告)号：US20240238394A1

公开(公告)日：2024-07-18

申请号：US18563822

申请日：2022-05-26

申请人： Modit Therapeutics Beijing Limited

发明人： Meifang ZHANG ,  Chengqi YI

IPC分类号： A61K38/52 ,  A61K31/7105 ,  A61K48/00 ,  C12N9/90 ,  C12N15/11

CPC分类号： A61K38/52 ,  A61K31/7105 ,  A61K48/005 ,  C12N9/90 ,  C12N15/11 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/531

摘要： Provided are methods, compositions, and systems for targeted pseudouridylation of RNA. In some aspects, provided are methods for editing a target RNA (e.g., mRNA) in a host cell, comprising introducing an engineered guide small nucleolar RNA (gsnoRNA) into the host cell, wherein the gsnoRNA recruits a DKC1 protein to modify a target uridine residue into a pseudouridine residue in the target RNA. In some embodiments, the DKC1 protein has cytoplasmic localization in the host cell.

公开(公告)号：US12031165B2

公开(公告)日：2024-07-09

申请号：US17477668

申请日：2021-09-17

申请人： Jiangnan University

发明人： Zhemin Zhou ,  Zhongmei Liu ,  Yufeng Liu ,  Li Zhou ,  Wenjing Cui ,  Junling Guo

IPC分类号： C12P13/04 ,  C12N9/88 ,  C12N9/90

CPC分类号： C12N9/88 ,  C12N9/90 ,  C12P13/04 ,  C12Y401/01012 ,  C12Y504/03009

摘要： Disclosed is a double enzyme tandem preparation method of L-2-aminobutyric acid, and belongs to the field of bioengineering. In the disclosure, recombinant Escherichia coli expressing L-glutamate mutase and recombinant Escherichia coli expressing L-aspartate-β-decarboxylase are separately cultured to obtain L-glutamate mutase and L-aspartate-β-decarboxylase. The two enzymes are added to a reaction system at a certain mass ratio, and L-glutamate is used as a substrate to carry out an enzyme reaction to prepare the L-2-aminobutyric acid. When the dosage of the L-aspartate-β-decarboxylase is 2 mg/mL, and the reaction time is 24 h, 8.5 mmol/L L-2-aminobutyric acid is produced by conversion, with a molar conversion rate of 85.00%. Compared with a chemical production method, the method disclosed by the disclosure has a safe production process and no environmental pollution. Compared with a multi-enzyme synthesis system with threonine as a substrate, the substrate is cheaper and the process is simpler.

公开(公告)号：US20240209403A1

公开(公告)日：2024-06-27

申请号：US18511394

申请日：2023-11-16

申请人： Antheia, Inc.

发明人： Christina D. SMOLKE ,  Isis TRENCHARD ,  Kristy M. HAWKINS ,  Catherine THODEY

IPC分类号： C12P17/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52

CPC分类号： C12P17/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y402/99

摘要： A method of producing promorphinan, morphinan, nal-opioid, and nor-opioid alkaloid products through the increased conversion of a promorphinan alkaloid to a morphinan alkaloid. The method comprises contacting the promorphinan alkaloid with at least one enzyme. Contacting the promorphinan alkaloid with the at least one enzyme converts the promorphinan alkaloid to a morphinan alkaloid.