公开(公告)号：US11905542B2

公开(公告)日：2024-02-20

申请号：US16648872

申请日：2018-09-25

申请人： DSM IP ASSETS B.V.

发明人： Nathalie Balch ,  Paul Blomquist ,  Reed Doten ,  Peter Houston ,  Ethan Lam ,  Jenna McMahon ,  Joshua Trueheart ,  Celine Viarouge

IPC分类号： C12P23/00

CPC分类号： C12P23/00

摘要： The present invention is related to a novel enzymatic process for production of retinyl esters, such as in particular retinyl long chain esters, via conversion of retinol, which process includes the use of enzymes having acyltransferase activity. Said process might be used for biotechnological production of vitamin A.

公开(公告)号：US12049658B2

公开(公告)日：2024-07-30

申请号：US16648879

申请日：2018-09-25

申请人： DSM IP ASSETS B.V.

发明人： Nathalie Balch ,  Paul Blomquist ,  Reed Doten ,  Peter Houston ,  Ethan Lam ,  Jenna Mcmahon ,  Joshua Trueheart ,  Celine Viarouge ,  René Marcel De Jong

IPC分类号： C12P23/00

CPC分类号： C12P23/00

摘要： The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.

公开(公告)号：US12018309B2

公开(公告)日：2024-06-25

申请号：US17051610

申请日：2019-05-21

申请人： DSM IP ASSETS B.V.

发明人： Christopher Mark Farrell ,  Lisa Ann Laprade ,  Otto Martin Lehmann ,  Joshua Trueheart ,  Bastien Jean Wolfgang Chevreux

IPC分类号： C12P33/02 ,  C12N1/16 ,  C12N9/02 ,  C12R1/645

CPC分类号： C12P33/02 ,  C12N1/165 ,  C12N9/0071 ,  C12R2001/645

摘要： The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US11578344B2

公开(公告)日：2023-02-14

申请号：US16647730

申请日：2018-09-25

申请人： DSM IP ASSETS B.V.

发明人： Nathalie Balch ,  Paul Blomquist ,  Reed Doten ,  Peter Houston ,  Ethan Lam ,  Jenna McMahon ,  Joshua Trueheart ,  Celine Viarouge

IPC分类号： C12P7/22 ,  C12N9/04 ,  C12N9/10 ,  C12P7/62

摘要： The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.

公开(公告)号：US20140315279A1

公开(公告)日：2014-10-23

申请号：US14105136

申请日：2013-12-12

申请人： DSM IP ASSETS b.v.

发明人： Richard B. Bailey ,  Kevin T. Madden ,  Joshua Trueheart

IPC分类号： C12N15/81

CPC分类号： C12N15/815 ,  C12N15/52 ,  C12N15/81 ,  C12P7/6463 ,  C12P7/6481 ,  C12P7/66

摘要： The present invention provides systems for producing engineered oleaginous yeast or fungi that express quinone derived compounds.

摘要翻译： 本发明提供用于生产表达醌衍生化合物的工程化含油酵母或真菌的系统。

公开(公告)号：US20140234928A1

公开(公告)日：2014-08-21

申请号：US14183268

申请日：2014-02-18

申请人： DSM IP ASSETS B.V.

发明人： Richard B. Bailey ,  Kevin T. Madden ,  Joshua Trueheart ,  Reed Doten ,  Maria Mayorga ,  Joshua Griffin Dunn ,  Dan Dueppen

IPC分类号： C12P23/00

CPC分类号： C12P23/00 ,  C12N15/52

摘要： The present invention provides systems for producing engineered oleaginous yeast or fungi that express retinolic compounds.

摘要翻译： 本发明提供用于生产工程化含油酵母或表达视黄醛化合物的真菌的系统。

公开(公告)号：US20200024609A1

公开(公告)日：2020-01-23

申请号：US16415257

申请日：2019-05-17

申请人： DSM IP Assets B.V.

发明人： Adam G. LAWRENCE ,  Peter Louis Houston ,  Jessica Leigh MCGRATH ,  Joshua Trueheart

IPC分类号： C12N15/81 ,  C12N1/16 ,  C12N15/10

摘要： The present invention is directed to a process for switching the mating type of a Yarrowia fungus strain into an opposite mating type, and to the use thereof to sexually cross two individual strains resulting in new strains.

公开(公告)号：US20150031868A1

公开(公告)日：2015-01-29

申请号：US14373571

申请日：2013-01-23

申请人： DSM IP ASSETS B.V.

发明人： Martin Lehmann ,  Joshua Trueheart ,  Priscilla Zwart Jens ,  Liang Wu ,  Viktor Marius Boer ,  Cornelis Maria Jacobus Sagt ,  Manoj Kumar ,  Bernard Meijrink ,  Marco Alexander Van Den Berg

IPC分类号： C12P19/56 ,  C12N9/10 ,  A23L1/236 ,  C07H15/256

CPC分类号： C12P19/56 ,  A23K20/10 ,  A23K20/121 ,  A23K20/163 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0073 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/007 ,  C12Y114/13078 ,  C12Y204/01035 ,  C12Y402/03019 ,  C12Y505/01013

摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

摘要翻译： 本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有海藻酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。 重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。

公开(公告)号：US12049617B2

公开(公告)日：2024-07-30

申请号：US17050626

申请日：2019-05-21

申请人： DSM IP Assets B.V.

发明人： Christopher Mark Farrell ,  Lisa Ann Laprade ,  Otto Martin Lehmann ,  Joshua Trueheart ,  Bastien Jean Wolfgang Chevreux

IPC分类号： C12N1/16 ,  C12N9/90 ,  C12P33/00

CPC分类号： C12N1/16 ,  C12N9/90 ,  C12P33/00

摘要： The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US10385308B2

公开(公告)日：2019-08-20

申请号：US14772137

申请日：2014-03-13

申请人： DSM IP Assets B.V.

发明人： Joshua Trueheart ,  Jessica McGrath

IPC分类号： C12P7/22 ,  C12N9/22 ,  C12Q1/68 ,  C12N1/08 ,  C12P23/00

摘要： The present invention relates to the use of a thermophilic nuclease for degrading nucleic acids in vivo and/or in situ, wherein the thermophilic nuclease is heterologous to the host cell and is produced by the host rather than being added exogenously. The present invention further relates to a genetically modified cell which was produced according to the above method. The present invention is particularly beneficial in inactivating the biological activity of recombinant DNA in biomass or biomass-derived products.