公开(公告)号：US12049617B2

公开(公告)日：2024-07-30

申请号：US17050626

申请日：2019-05-21

申请人： DSM IP Assets B.V.

发明人： Christopher Mark Farrell ,  Lisa Ann Laprade ,  Otto Martin Lehmann ,  Joshua Trueheart ,  Bastien Jean Wolfgang Chevreux

IPC分类号： C12N1/16 ,  C12N9/90 ,  C12P33/00

CPC分类号： C12N1/16 ,  C12N9/90 ,  C12P33/00

摘要： The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US12018309B2

公开(公告)日：2024-06-25

申请号：US17051610

申请日：2019-05-21

申请人： DSM IP ASSETS B.V.

发明人： Christopher Mark Farrell ,  Lisa Ann Laprade ,  Otto Martin Lehmann ,  Joshua Trueheart ,  Bastien Jean Wolfgang Chevreux

IPC分类号： C12P33/02 ,  C12N1/16 ,  C12N9/02 ,  C12R1/645

CPC分类号： C12P33/02 ,  C12N1/165 ,  C12N9/0071 ,  C12R2001/645

摘要： The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.