公开(公告)号：US12060497B2

公开(公告)日：2024-08-13

申请号：US16903028

申请日：2020-06-16

申请人： Toyota Motor Corporation ,  Regents of the University of Minnesota

发明人： Hongfei Jia ,  Ping Wang ,  Liting Zhang ,  Andreas Buthe ,  Xueyan Zhao ,  Songtao Wu ,  Masahiko Ishii ,  Minjuan Zhang

IPC分类号： C09D189/00 ,  A61K47/60 ,  C08G65/333 ,  C09D5/02 ,  C09D5/16 ,  C09D7/40 ,  C09D175/16 ,  C12N9/14 ,  C12N9/20 ,  C12N9/26 ,  C12N9/54 ,  C12N9/96 ,  C12N11/06 ,  C12N11/089

CPC分类号： C09D189/00 ,  A61K47/60 ,  C08G65/33337 ,  C09D5/024 ,  C09D5/16 ,  C09D7/40 ,  C09D175/16 ,  C12N9/14 ,  C12N9/20 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/54 ,  C12N9/96 ,  C12N11/06 ,  C12N11/089 ,  C08L2203/02 ,  C08L2205/02

摘要： Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme capable of enzymatically degrading a component of an organic stain, optionally a lipase or a lysozyme, and optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or bacterial organic material.

公开(公告)号：US12043834B2

公开(公告)日：2024-07-23

申请号：US17525283

申请日：2021-11-12

申请人： Qilu University of Technology

发明人： Hongling Liu ,  Tengfei Wang ,  Ruiming Wang ,  Xinyi Zhang

IPC分类号： C12N15/52 ,  C12N9/10 ,  C12N9/26 ,  C12N9/90 ,  C12N11/18 ,  C12N15/03 ,  C12N15/75

CPC分类号： C12N15/52 ,  C12N9/1051 ,  C12N9/2408 ,  C12N9/90 ,  C12N11/18 ,  C12N15/03 ,  C12N15/75 ,  C12Y302/01141 ,  C12Y504/99015

摘要： The present disclosure relates to a method for constructing a trehalose polyenzyme complex in vitro by mediation of an artificial scaffold protein, which mainly comprises the following steps: constructing a recombinant bacterium WB800n-ScafCCR for self-assembled scaffold protein module; constructing a recombinant bacterium WB800n-P43-phoD -treY-Ccdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-treZ-Ctdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-cgt-Rfdoc for self-assembled catalytic module; secretorily expressing the recombinant bacteria and self-assembling in vitro to obtain a recombinant trehalose multi-enzyme complex. The trehalose multi-enzyme complex constructed by the method of the present disclosure has a higher catalytic efficiency in preparing trehalose than that of mixed free enzymes, and the method can be used for production of high quality trehalose after immobilization with cellulose microspheres.

公开(公告)号：US20240101991A1

公开(公告)日：2024-03-28

申请号：US17936076

申请日：2022-09-28

申请人： NOVOZYMES A/S

发明人： Marc Dominique Morant ,  Tomoko Matsui ,  Kirk Matthew Schnorr ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Zhengfang Kang

IPC分类号： C12N9/26 ,  C12N15/81 ,  C12P7/06

CPC分类号： C12N9/2408 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01028

摘要： The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.

公开(公告)号：US20230313261A1

公开(公告)日：2023-10-05

申请号：US18041842

申请日：2021-08-19

申请人： 3M INNOVATIVE PROPERTIES COMPANY

发明人： Joshua D. Erickson ,  Narendranath Bhokisham ,  Tonya D. Bonilla ,  Minghua Dai

IPC分类号： C12Q1/18 ,  C12N9/26 ,  A61L2/28 ,  A61L2/07 ,  C07K14/32

CPC分类号： C12Q1/18 ,  C12N9/2408 ,  C12Y302/0102 ,  A61L2/28 ,  A61L2/07 ,  C07K14/32 ,  C07K2319/61

摘要： An article for assessing efficacy of a sterilization process is provided. The article includes a housing, a plurality of genetically-modified spore-forming test microorganisms disposed in the housing, a liquid medium disposed in an openable container that is disposed in or attached to the housing, and an enzyme substrate disposed in the housing or in the openable container. The genetically-modified test microorganism comprises a functional fusion gene that encodes a non-naturally occurring chimeric protein, the chimeric protein comprising a first segment and a second segment that is contiguous with the first segment. The first segment comprises at least a portion of a first polypeptide that is normally found in spores and the second segment comprises a second polypeptide having a detectable enzymatic activity. The enzyme substrate is capable of reacting with the detectable enzyme activity to form a detectable product. A method of using the test microorganisms is also provided.

公开(公告)号：US20190203239A1

公开(公告)日：2019-07-04

申请号：US16356138

申请日：2019-03-18

申请人： Battelle Energy Alliance, LLC

发明人： David N. Thompson ,  William A. Apel ,  Vicki S. Thompson ,  David W. Reed ,  Jeffrey A. Lacey ,  Emily D. Henriksen

IPC分类号： C12P19/14 ,  C12N9/10 ,  C12P19/02 ,  C12P19/00 ,  C12N15/52 ,  C12N9/88 ,  C12N9/80 ,  C12N9/24 ,  C12N9/38 ,  C12N9/40 ,  C12N9/42 ,  C12N9/34 ,  C12N9/26 ,  C12N9/18 ,  C12N9/16

CPC分类号： C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

公开(公告)号：US20190194679A1

公开(公告)日：2019-06-27

申请号：US16086416

申请日：2017-03-20

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： Henry Daniell

IPC分类号： C12N15/82 ,  C12N9/26 ,  A61P31/14 ,  A61K39/12 ,  A61P1/02 ,  A61K9/68 ,  C07K14/47 ,  C07K14/755 ,  C07K14/65 ,  C12N7/00

CPC分类号： C12N15/8258 ,  A61K9/0058 ,  A61K39/12 ,  A61K2039/542 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/55577 ,  A61K2039/575 ,  A61K2039/6037 ,  A61P1/02 ,  A61P31/14 ,  C07K14/4723 ,  C07K14/65 ,  C07K14/755 ,  C07K2319/00 ,  C12N1/00 ,  C12N7/00 ,  C12N9/24 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2454 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8257 ,  C12N2770/32634 ,  C12Y302/01084

摘要： Methods for improving transgene in chloroplasts are disclosed along with improved transgenes so produced and methods of use thereof for the treatment of disease. Specifically, the methods comprising analyzing the native sequence of a nucleic acid encoding a protein of interest and replacing codons in said sequence with those preferentially used in psbA genes in chloroplasts in higher plants.

公开(公告)号：US10016491B2

公开(公告)日：2018-07-10

申请号：US14936290

申请日：2015-11-09

申请人： Halozyme, Inc.

发明人： Louis H. Bookbinder ,  Anirban Kundu ,  Gregory I. Frost

IPC分类号： A61K38/47 ,  A61K39/395 ,  C12N9/26 ,  A61K45/06 ,  A61K39/00

CPC分类号： A61K38/47 ,  A61K39/395 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C12N9/2408 ,  C12Y302/01035 ,  A61K2300/00

摘要： The invention relates to the discovery of novel soluble neutral active Hyaluronidase Glycoproteins (sHASEGPs), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. The invention further comprises sialated and pegylated form of a recombinant sHASEGP to enhance stability and serum pharmacokinetics over naturally occurring slaughterhouse enzymes. Further described are suitable formulations of a substantially purified recombinant sHASEGP glycoprotein derived from a eukaryotic cell that generate the proper glycosylation required for its optimal activity.

公开(公告)号：US09889203B2

公开(公告)日：2018-02-13

申请号：US14762647

申请日：2014-03-14

申请人： AMICUS THERAPEUTICS, INC.

发明人： Hung Do

IPC分类号： A61K47/48 ,  C07K14/65 ,  C12N9/16 ,  C12N9/20 ,  C12N9/24 ,  C12N9/26 ,  C12N9/42 ,  C12N9/40 ,  A61K38/30 ,  A61K38/47 ,  C12N9/96

CPC分类号： A61K47/64 ,  A61K38/30 ,  A61K38/47 ,  A61K47/60 ,  C07K14/65 ,  C07K2319/00 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2434 ,  C12N9/2465 ,  C12N9/2488 ,  C12N9/96 ,  C12Y302/0102

摘要： Disclosed herein are methods of chemical conjugation comprising contacting a lysosomal enzyme with a first crosslinking agent to introduce aldehyde groups; contacting a lysosomal targeting peptide with a second crosslinking agent to introduce a hydrazide group at the N-terminal residue; contacting the lysosomal enzyme with aldehyde groups of step a. with the lysosomal targeting peptide with a hydrazide group at the N-terminal residue of step b; and forming a lysosomal enzyme-lysosomal targeting peptide conjugate.

公开(公告)号：US09873868B2

公开(公告)日：2018-01-23

申请号：US13403783

申请日：2012-02-23

申请人： Dwight D. Koeberl ,  Baodong Sun

发明人： Dwight D. Koeberl ,  Baodong Sun

IPC分类号： A01N63/00 ,  C12N9/24 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C07K1/00 ,  A61K48/00 ,  C12N9/26

CPC分类号： C12N9/2408 ,  C07K2319/02 ,  C07K2319/036

摘要： Provided are isolated nucleic acids for expressing lysosomal polypeptides such as lysosomal acid α-glucosidase (GAA) and vectors comprising the same. The invention provides an isolated nucleic acid encoding a chimeric polypeptide comprising a secretory signal sequence operably linked to a lysosomal polypeptide. The invention also provides an isolated nucleic acid comprising a coding region encoding a GAA and a GAA 3′ untranslated region (UTR), wherein the GAA 3′ UTR comprises a deletion therein. The invention further provides an isolated nucleic acid comprising a coding region encoding a GAA and a 3′ UTR wherein the 3′ UTR is less than about 200 nucleotides in length and comprises a segment that is heterologous to the GAA coding region. Also provided are methods of making and using delivery vectors encoding lysosomal polypeptides to produce the lysosomal polypeptide to treat subjects afflicted with a deficiency in the lysosomal polypeptide.

公开(公告)号：US20180009904A1

公开(公告)日：2018-01-11

申请号：US15529355

申请日：2015-11-20

申请人： Shire Human Genetic Therapies, Inc.

发明人： Vinayaka KOTRAIAH ,  Bohong ZHANG ,  Michael F. CONCINO

IPC分类号： C07K16/40 ,  C12N9/26 ,  C12N9/24 ,  A61K38/00

CPC分类号： C07K16/40 ,  A61K38/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00 ,  C07K2319/06 ,  C07K2319/33 ,  C07K2319/50 ,  C07K2319/70 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/2408 ,  C12Y302/0102 ,  C12Y302/0105 ,  C12Y304/21061

摘要： The invention provides compositions and methods for effective lysosomal targeting mediated by PCSK9. In particular, the compositions and methods provided by the invention may be used to treat lysosomal storage diseases such as Pompe Disease and Sanfilippo Syndrome Type B, and they may be used for targeting lysosomal enzymes to the various muscles of the human body.