知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

39 条记录 (耗时 0.02 秒)

    Method for selecting genetically transformed cells
    2.
    发明申请
    Method for selecting genetically transformed cells 失效
    选择遗传转化细胞的方法

    公开(公告)号：US20050084971A1

    公开(公告)日：2005-04-21

    申请号：US10494651

    申请日：2002-11-06

    申请人： Josephus Christianus Smeekens ,  Henriette Schlupmann

    发明人： Josephus Christianus Smeekens ,  Henriette Schlupmann

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/82 ,  C12Q1/04 ,  C12N15/74

    CPC分类号： C12Y302/01028 ,  C12N9/2402 ,  C12N15/8209

    摘要： An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-transformed cells. The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequence to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non-transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.

    摘要翻译： 用于从由转化和非转化细胞组成的群体中选择转化细胞的环境友好和无毒的方法。 该方法包括以下步骤：a）向细胞中加入至少一种所需的核苷酸序列和至少一种选择核苷酸序列以获得遗传转化的细胞，其中所述选择核苷酸序列包含编​​码参与所述核苷酸序列的蛋白质的区域 代谢海藻糖; b）将具有转化和未转化细胞的群体放置成与海藻糖和/或其衍生物接触; 和c）基于转化细胞的能力来代替海藻糖和/或衍生物从群体中选择转化的细胞。

    RECOMBINANT THERMOSTABLE ENZYME WHICH FORMS NON-REDUCING SACCHARIDE FROM REDUCING AMYLACEOUS SACCHARIDE
    4.
    发明申请
    RECOMBINANT THERMOSTABLE ENZYME WHICH FORMS NON-REDUCING SACCHARIDE FROM REDUCING AMYLACEOUS SACCHARIDE 审中-公开
    重构可热稳定酶，其形式是从减少AMYLACEOUS SACCHARIDE中减少SACCHARIDE

    公开(公告)号：US20020102696A1

    公开(公告)日：2002-08-01

    申请号：US09419305

    申请日：1999-10-15

    发明人： KAZUHIKO MARUTA ,  MICHIO KUBOTA ,  TOSHIYUKI SUGIMOTO

    IPC分类号： C12N011/08

    CPC分类号： C12P19/12 ,  C12N9/2414 ,  C12N2310/111 ,  C12N2310/33 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01028

    摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 69,000-79,000 daltons and a pI of about 5.4-6.4, and forms non-reducing saccharides having a trehalose structure as an end unit from reducing amylaceous saccharides having a degree of glucose polymerization of at least 3. The enzyme has satisfactorily high thermostability, i.e. it is substantially not inactivated even when incubated in an aqueous solution (pH 7.0) at 85null C. for 60 min, and this facilitates the production of such non-reducing saccharides on an industrial scale and in a satisfactorily-high yield.

    摘要翻译： 公开了一种重组热稳定酶，其分子量为约69,000-79,000道尔顿，pI为约5.4-6.4，并形成具有海藻糖结构的非还原性糖类作为末端单元，所述还原糖具有还原具有葡萄糖聚合度的淀粉糖 该酶具有令人满意的高的热稳定性，即使其在85℃下在水溶液（pH7.0）中孵育60分钟时基本上也不失活，这有利于生产这种非还原性糖类 工业规模，产量令人满意。

    ">
    10.
    发明授权
    "Alga species lobsphaera TM-33 (ATCC 75630) which is useful for preparing trehalase" 失效
    “可用于制备海藻糖酶的藻类物种lobsphaera TM-33（ATCC 75630）”

    公开(公告)号：US5529927A

    公开(公告)日：1996-06-25

    申请号：US231318

    申请日：1994-04-22

    申请人： Sumio Kitahata ,  Hirofumi Nakano ,  Tsutomu Washino ,  Masamitsu Moriwaki

    发明人： Sumio Kitahata ,  Hirofumi Nakano ,  Tsutomu Washino ,  Masamitsu Moriwaki

    IPC分类号： C12N1/12 ,  C12N9/24 ,  C12P19/12 ,  C12R1/89

    CPC分类号： C12P19/12 ,  C12N9/2402 ,  C12Y302/01028 ,  Y10S435/946

    摘要： Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein.

    摘要翻译： 具有将α，α'-海藻糖，2,2'-二脱氧-α，α'-海藻糖和2-脱氧-α，α'-海藻糖水解成各自组成糖的性质的新型海藻糖酶，同时不起作用 新海藻糖，乳糖，麦芽糖，头孢噻吩和蔗糖; 其最适pH为5〜6; 其最适温度为65℃。 对于加热至65℃是稳定的; 其通过凝胶过滤测量的分子量为40万至500,000，而通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳测量的亚单位的分子量为18万至25万; 通过等电聚焦测量的等电点为2.7; 它是糖蛋白。