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1.发明授权Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide 失效从非还原性糖释放海藻糖的重组热稳定酶
公开(公告)号：US6027918A
公开(公告)日：2000-02-22
申请号：US798269
申请日：1997-02-11
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/24 , C12N15/52 , C12N15/56 , C12N15/70 , C12P1/04 , C12P19/12 , C12P19/14 , C12P21/00 , C12R1/01 , C12R1/19 , C12P21/02 , C12N15/31
CPC分类号： C12Y302/01141 , C12N9/1051 , C12P19/12 , C12P19/14 , C12Y204/01064 , Y10S435/822 , Y10S530/825
摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.
摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 至少3.酶具有令人满意的高热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有利于在工业规模上生产海藻糖， 以令人满意的高产量。
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2.发明授权DNA encoding enzyme, recombinant DNA and enzyme, transformant, and their preparation and uses 失效DNA编码酶，重组DNA和酶，转化体及其制备和用途
公开(公告)号：US5834287A
公开(公告)日：1998-11-10
申请号：US605501
申请日：1996-02-26
CPC分类号： C12Y302/01141 , C12N9/2402 , C12P19/14 , A61K38/00
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了编码从海藻糖结构作为末端单元，葡萄糖聚合度为3以上，重组DNA，酶，转化体及其制备和用途的非还原性糖类释放海藻糖的DNA。 这些促进了相对容易且成本低的海藻糖的工业规模生产，并且由此获得的海藻糖可以令人满意地用于各种食品，化妆品和药物中。
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3.发明授权
公开(公告)号：US5716813A
公开(公告)日：1998-02-10
申请号：US607321
申请日：1996-02-26
CPC分类号： C12Y302/01141 , C12N9/2402 , C12P19/14 , A61K38/00
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
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4.发明授权DNA encoding enzyme, recombinant DNA and enzyme, transformant, and their preparations and uses 失效DNA编码酶，重组DNA和酶，转化体及其制备和用途
公开(公告)号：US5556781A
公开(公告)日：1996-09-17
申请号：US399646
申请日：1995-03-07
CPC分类号： C12Y302/01141 , C12N9/2402 , C12P19/14 , A61K38/00
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了编码从海藻糖结构作为末端单元，葡萄糖聚合度为3以上，重组DNA，酶，转化体及其制备和用途的非还原性糖类释放海藻糖的DNA。 这些促进了相对容易且成本低的海藻糖的工业规模生产，并且由此获得的海藻糖可以令人满意地用于各种食品，化妆品和药物中。
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5.发明授权Bacteria with increased trehalose production and method for using the same in bioremediation 失效增加海藻糖生产的细菌和在生物修复中使用该细菌的方法公开(公告)号：US08481026B1
公开(公告)日：2013-07-09
申请号：US12762023
申请日：2010-04-16
CPC分类号： C12N15/52 , B09C1/10 , C12N9/1051 , C12N9/16 , C12N9/2402 , C12Y204/01015 , C12Y204/01245 , C12Y301/03012 , C12Y302/01141 , C12Y504/99015 , C12Y504/99016
摘要： Bacteria with improved survival under toxic and oxidative stress and methods of using the same for bioremediation are described. Many bacteria such as Escherichia coli have been found to naturally reduce toxic pollutants into a less toxic form, such as by reducing Chromium (VI) to Chromium (III). Reducing such toxins generates damaging reactive oxygen species, so it is important to find a defense for these bacteria against the associated oxidative stress. Trehalose is a small biomolecule that has been shown to protect bacteria from various types of stress by accumulating within the cells. The present invention is directed to bacteria genetically modified to overexpress trehalose biosynthesis genes. The bacteria demonstrate improved viability when challenged with toxins and oxidative stress. The present invention provides inexpensive and beneficial bacteria and methods for environmental remediation.
摘要翻译： 描述了在有毒和氧化应激下具有改善的存活的细菌以及将其用于生物修复的方法。 已经发现许多细菌如大肠杆菌自然地将毒性污染物减少成毒性较小的形式，例如通过将铬（VI）还原成铬（III）。 减少这种毒素会产生有害的活性氧，所以重要的是找到这些细菌抵抗相关的氧化应激的防御。 海藻糖是一种小的生物分子，已被证明通过在细胞内积累来保护细菌免受各种类型的压力。 本发明涉及遗传修饰以过表达海藻糖生物合成基因的细菌。 当用毒素和氧化应激攻击时，细菌表现出改善的活力。 本发明提供廉价和有益的细菌和环境修复方法。
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公开(公告)号：US6150153A
公开(公告)日：2000-11-21
申请号：US888158
申请日：1997-07-03
IPC分类号： A23C9/123 , A21D2/18 , A23B4/02 , A23C9/152 , A23G3/34 , A23G3/48 , A23G4/00 , A23J3/16 , A23L1/19 , A23L1/202 , A23L1/236 , A23L1/30 , A23L1/32 , A23L2/39 , A61K8/04 , A61K8/60 , A61K8/72 , A61K8/73 , A61K31/70 , A61K36/25 , A61K38/00 , A61K47/26 , A61P3/00 , A61P17/00 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C07H3/04 , C12N9/24 , C12P19/12 , C12P19/14 , C12R1/01 , C12N1/20
CPC分类号： C12Y302/01141 , A61K8/60 , A61Q11/00 , A61Q17/04 , A61Q19/02 , A61Q7/00 , C12N9/2402 , C12P19/12 , C12P19/14 , Y10S435/822
摘要： Disclosed are novel thermostable trehalose-releasing enzyme, and its preparations and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of hydrolyzing at a temperature of over 55.degree. C. the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. Trehalose and compositions containing the same are extensively useful in food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了新型热稳定海藻糖释放酶及其制备和用途。 该酶可从微生物的培养物获得，例如硫酸亚硫酸杆菌（ATCC 33909和ATCC 49426）和溶血S螨（Sulfolobus solfataricus）（ATCC 35091和ATCC 35092），并且能够在超过55℃的温度下水解，海藻糖部分 并且具有海藻糖结构的非还原性糖中的剩余的糖基部分作为末端单元，并且具有3或更高的葡萄糖聚合度。 海藻糖和含有它们的组合物在食品，化妆品和药物中广泛使用。
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7.发明授权Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide 失效从非还原性糖释放海藻糖的重组热稳定酶
公开(公告)号：US5856146A
公开(公告)日：1999-01-05
申请号：US505377
申请日：1995-07-21
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/24 , C12N15/52 , C12N15/56 , C12N15/70 , C12P1/04 , C12P19/12 , C12P19/14 , C12P21/00 , C12R1/01 , C12R1/19 , C07K14/195 , C12P19/18
CPC分类号： C12Y302/01141 , C12N9/1051 , C12P19/12 , C12P19/14 , C12Y204/01064 , Y10S435/822 , Y10S530/825
摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industial scale and in a satisfactorily-high yield.
摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 该酶具有令人满意的高的热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有利于在工业规模上生产海藻糖， 以令人满意的高产量。
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8.发明授权
公开(公告)号：US5830715A
公开(公告)日：1998-11-03
申请号：US961240
申请日：1997-10-30
CPC分类号： C12Y302/01141 , C12N9/2402 , C12P19/14 , A61K38/00
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
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公开(公告)号：US5591612A
公开(公告)日：1997-01-07
申请号：US443352
申请日：1995-05-17
IPC分类号： A21D2/18 , A21D8/04 , A23C9/00 , A23C9/13 , A23C9/156 , A23G1/00 , A23G1/40 , A23G3/00 , A23G3/34 , A23G3/42 , A23G4/00 , A23G4/10 , A23L1/09 , A23L1/187 , A23L1/20 , A23L1/236 , A23L1/30 , A23L1/314 , A23L1/318 , A23L1/32 , A23L1/325 , A23L2/39 , A61K8/60 , A61K31/70 , A61Q7/00 , A61Q11/00 , A61Q19/00 , C07H3/04 , C12G3/02 , C12N1/20 , C12N9/24 , C12N9/90 , C12P19/12 , C12P19/14 , C12P19/24 , C12R1/06 , C12R1/13 , C12R1/265 , C12R1/41 , C13K20060101 , C13K13/00 , C12N9/10 , C12N9/14 , C12N9/26
CPC分类号： C13K13/00 , A21D2/181 , A21D2/186 , A21D8/042 , A23C9/005 , A23C9/1307 , A23C9/156 , A23G1/40 , A23G3/346 , A23G3/42 , A23G4/10 , A23L11/05 , A23L13/428 , A23L13/72 , A23L15/20 , A23L15/30 , A23L17/70 , A23L2/39 , A23L27/33 , A23L29/35 , A23L9/12 , A61K8/60 , A61Q19/00 , C12G3/02 , C12N9/2457 , C12N9/90 , C12P19/12 , C12P19/14 , C12P19/24 , C12Y302/01141 , A23G2200/02 , A23G2200/06 , A61Q11/00 , A61Q17/04 , A61Q19/02 , A61Q7/00
摘要： Disclosed is a trehalose-releasing enzyme which specifically hydrolyzes the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. The molecular weight of the enzyme is about 57,000 to 68,000 daltons on SDS-PAGE, and the isoelectric point is about 3.3 to 4.6 on isoelectrophoresis. The enzyme is useful in an industrial-scale preparation of trehalose, and the trehalose prepared therewith can be readily incorporated into food products, as well as cosmetic- and pharmaceutical-compositions.
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10.发明授权公开(公告)号：US12043834B2
公开(公告)日：2024-07-23
申请号：US17525283
申请日：2021-11-12
发明人： Hongling Liu , Tengfei Wang , Ruiming Wang , Xinyi Zhang
CPC分类号： C12N15/52 , C12N9/1051 , C12N9/2408 , C12N9/90 , C12N11/18 , C12N15/03 , C12N15/75 , C12Y302/01141 , C12Y504/99015
摘要： The present disclosure relates to a method for constructing a trehalose polyenzyme complex in vitro by mediation of an artificial scaffold protein, which mainly comprises the following steps: constructing a recombinant bacterium WB800n-ScafCCR for self-assembled scaffold protein module; constructing a recombinant bacterium WB800n-P43-phoD -treY-Ccdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-treZ-Ctdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-cgt-Rfdoc for self-assembled catalytic module; secretorily expressing the recombinant bacteria and self-assembling in vitro to obtain a recombinant trehalose multi-enzyme complex. The trehalose multi-enzyme complex constructed by the method of the present disclosure has a higher catalytic efficiency in preparing trehalose than that of mixed free enzymes, and the method can be used for production of high quality trehalose after immobilization with cellulose microspheres.
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