公开(公告)号：US5856146A

公开(公告)日：1999-01-05

申请号：US505377

申请日：1995-07-21

申请人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

发明人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/24 ,  C12N15/52 ,  C12N15/56 ,  C12N15/70 ,  C12P1/04 ,  C12P19/12 ,  C12P19/14 ,  C12P21/00 ,  C12R1/01 ,  C12R1/19 ,  C07K14/195 ,  C12P19/18

CPC分类号： C12Y302/01141 ,  C12N9/1051 ,  C12P19/12 ,  C12P19/14 ,  C12Y204/01064 ,  Y10S435/822 ,  Y10S530/825

摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industial scale and in a satisfactorily-high yield.

摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 该酶具有令人满意的高的热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有利于在工业规模上生产海藻糖， 以令人满意的高产量。

公开(公告)号：US20120301563A1

公开(公告)日：2012-11-29

申请号：US13289950

申请日：2011-11-04

申请人： John Aikens ,  Robert J. Turner

发明人： John Aikens ,  Robert J. Turner

IPC分类号： C12N1/12 ,  C12N1/13 ,  A61K35/74 ,  A61P17/00 ,  A23L1/48 ,  C11B1/00 ,  C08B37/00 ,  C10L1/18 ,  C10L1/19 ,  C12P19/14 ,  A61P19/02

CPC分类号： C12P19/12 ,  C12M21/02 ,  C12M23/24 ,  C12M23/26 ,  C12M25/02 ,  C12M33/00 ,  C12M41/06 ,  C12N1/12 ,  C12N9/1051 ,  C12N9/1066 ,  C12N9/16 ,  C12N15/74 ,  C12N15/80 ,  C12Y204/01014 ,  C12Y204/01015 ,  C12Y204/01036 ,  C12Y204/01064 ,  C12Y204/01213 ,  C12Y301/03012 ,  C12Y301/03024

摘要： Provided herein are methods of producing and processing a feedstock from a photosynthetic microorganism. Also provided are various transgenic photosynthetic microorganism and photobioreactors for use in the methods.

摘要翻译： 本文提供了从光合微生物生产和加工原料的方法。 还提供了用于该方法的各种转基因光合微生物和光生物反应器。

公开(公告)号：US06346394B1

公开(公告)日：2002-02-12

申请号：US09055210

申请日：1998-04-06

申请人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

发明人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

IPC分类号： C12N924

CPC分类号： C12Y302/01141 ,  C12N9/1051 ,  C12P19/12 ,  C12P19/14 ,  C12Y204/01064 ,  Y10S435/822 ,  Y10S530/825

摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85° C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.

摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 该酶具有令人满意的高的热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有助于以工业规模生产海藻糖， 以令人满意的高产量。

公开(公告)号：US20130302857A1

公开(公告)日：2013-11-14

申请号：US13885789

申请日：2011-12-09

申请人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

发明人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

IPC分类号： C12N9/10

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/12 ,  C12P19/18 ,  C12Y204/01064

摘要： The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogues and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.

摘要翻译： 本发明涉及可用于工业生产海藻糖类似物和糖基磷酸盐的海藻糖磷酸酶。 更具体地，本发明公开了在特定氨基酸区域突变的海藻糖磷酸酶。 这些特异性突变导致酶修饰的底物特异性。 此外，本发明公开了一种来自海洋生物体Caldanaerobacter subterraneus的野生型海藻糖磷酸化酶及其突变型，其具有高度热稳定性并具有广泛的受体和供体特异性。

公开(公告)号：US20020068349A1

公开(公告)日：2002-06-06

申请号：US09381624

申请日：1999-12-15

发明人： SUEHARU HORINOUCHI ,  KOHKI SAITOH ,  EISAKU TAKAHASHI

IPC分类号： C12N009/16 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC分类号： C12N9/1051 ,  C12P19/18 ,  C12Y204/01064

摘要： A novel recombinant trehalose phosphorylase is provided. A gene encoding trehalose phosphorylase has been discovered. A vector containing the gene is constructed, a transformant is prepared, and recombinant trehalose phosphorylase is produced using the transformant. Trehalose phosphorylase can be produced in an industrial scale. Trehalose can be manufactured advantageously in an industry scale from D-glucose and null-D-glucose 1-phosphoric acid using this enzyme.

摘要翻译： 提供了一种新的重组海藻糖磷酸化酶。 已经发现编码海藻糖磷酸化酶的基因。 构建含有该基因的载体，制备转化体，使用该转化体产生重组海藻糖磷酸化酶。 海藻糖磷酸酶可以工业规模生产。 可以使用该酶从D-葡萄糖和α-D-葡萄糖1-磷酸以工业规模有利地制造海藻糖。

公开(公告)号：US20230193220A1

公开(公告)日：2023-06-22

申请号：US17811933

申请日：2022-07-12

申请人： C-Lecta GmbH ,  New Matterhorn, LLC

发明人： Christopher David BAYER ,  Andreas VOGEL ,  Marc STRUHALLA ,  Birgit BRUCHER

IPC分类号： C12N9/10 ,  C12N9/92 ,  C12P19/12 ,  C12P19/18 ,  C12P19/24

CPC分类号： C12N9/1051 ,  C12N9/92 ,  C12P19/12 ,  C12P19/18 ,  C12P19/24 ,  C12Y204/01007 ,  C12Y204/01064

摘要： The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.

公开(公告)号：US09102922B2

公开(公告)日：2015-08-11

申请号：US13885789

申请日：2011-12-09

申请人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

发明人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

IPC分类号： C12N9/10 ,  C12P19/12 ,  C12P19/18 ,  C12P19/02

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/12 ,  C12P19/18 ,  C12Y204/01064

摘要： The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogs and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.

摘要翻译： 本发明涉及可用于工业生产海藻糖类似物和糖基磷酸盐的海藻糖磷酸酶。 更具体地，本发明公开了在特定氨基酸区域突变的海藻糖磷酸酶。 这些特异性突变导致酶修饰的底物特异性。 此外，本发明公开了一种来自海洋生物体Caldanaerobacter subterraneus的野生型海藻糖磷酸化酶及其突变型，其具有高度热稳定性并具有广泛的受体和供体特异性。

公开(公告)号：US6027918A

公开(公告)日：2000-02-22

申请号：US798269

申请日：1997-02-11

申请人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

发明人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/24 ,  C12N15/52 ,  C12N15/56 ,  C12N15/70 ,  C12P1/04 ,  C12P19/12 ,  C12P19/14 ,  C12P21/00 ,  C12R1/01 ,  C12R1/19 ,  C12P21/02 ,  C12N15/31

CPC分类号： C12Y302/01141 ,  C12N9/1051 ,  C12P19/12 ,  C12P19/14 ,  C12Y204/01064 ,  Y10S435/822 ,  Y10S530/825

摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.

摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 至少3.酶具有令人满意的高热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有利于在工业规模上生产海藻糖， 以令人满意的高产量。