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    GALACTOSE TO TAGATOSE ISOMERIZATION AT MODERATE TEMPERATURES WITH HIGH CONVERSION AND PRODUCTIVITY
    1.
    发明公开
    GALACTOSE TO TAGATOSE ISOMERIZATION AT MODERATE TEMPERATURES WITH HIGH CONVERSION AND PRODUCTIVITY 审中-公开

    公开(公告)号：US20240240215A1

    公开(公告)日：2024-07-18

    申请号：US18492264

    申请日：2023-10-23

    申请人： Trustees of Tufts College

    发明人： Nikhil U. Nair ,  Josef R. Bober

    IPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02

    CPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02

    摘要： Disclosed are components and methods for preparing tagatose from galactose via isomerization reactions using engineered components. The engineered components include microbial cells and methods for preparing microbial cells that have been engineered to catalyze isomerization of galactose to tagatose, in which the microbial cells express cytoplasmically an exogenous L-arabinose isomerase enzyme. The disclosed microbial cells may further be modified for use in methods for preparing tagatose from galactose via isomerization reactions where the microbial cells are treated with reagents that permeabilize the cells. The disclosed methods enable isomerization reactions of galactose to tagatose at relatively high rates, high conversions, and elevated temperatures.

    Galactose to tagatose isomerization at moderate temperatures with high conversion and productivity
    2.
    发明授权
    Galactose to tagatose isomerization at moderate temperatures with high conversion and productivity 有权

    公开(公告)号：US11834692B2

    公开(公告)日：2023-12-05

    申请号：US17168499

    申请日：2021-02-05

    申请人： Trustees of Tufts College

    发明人： Nikhil U. Nair ,  Josef R. Bober

    IPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02

    CPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02

    摘要： Disclosed are components and methods for preparing tagatose from galactose via isomerization reactions using engineered components. The engineered components include microbial cells and methods for preparing microbial cells that have been engineered to catalyze isomerization of galactose to tagatose, in which the microbial cells express cytoplasmically an exogenous L-arabinose isomerase enzyme. The disclosed microbial cells may further be modified for use in methods for preparing tagatose from galactose via isomerization reactions where the microbial cells are treated with reagents that permeabilize the cells. The disclosed methods enable isomerization reactions of galactose to tagatose at relatively high rates, high conversions, and elevated temperatures.

    Glucose isomerase
    3.
    发明授权
    Glucose isomerase 有权

    公开(公告)号：US11674129B2

    公开(公告)日：2023-06-13

    申请号：US17303882

    申请日：2021-06-09

    申请人： C-LEcta GmbH ,  New Matterhorn, LLC

    发明人： Birgit Brucher ,  Andreas Vogel ,  Hanna Maria Dudek ,  Rico Czaja

    IPC分类号： C12N9/92 ,  C12P19/02 ,  C12P19/24

    CPC分类号： C12N9/92 ,  C12P19/02 ,  C12P19/24

    摘要： The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.

    METHOD OF PRODUCING HIGH PURITY D-PSICOSE
    5.
    发明申请
    METHOD OF PRODUCING HIGH PURITY D-PSICOSE 审中-公开

    公开(公告)号：US20180327796A1

    公开(公告)日：2018-11-15

    申请号：US15776930

    申请日：2016-04-12

    申请人： CJ CHEILJEDANG CORPORATION

    发明人： Joo Hang LEE ,  Min Hoe KIM ,  Seong Bo KIM ,  Seung Won PARK

    IPC分类号： C12P19/02 ,  B01D15/36 ,  A23L27/30 ,  C12P19/24 ,  C13K13/00

    CPC分类号： C12P19/02 ,  A23L27/33 ,  A23V2002/00 ,  B01D15/361 ,  C07H1/00 ,  C07H3/02 ,  C12P19/24 ,  C13K13/007

    摘要： Disclosed herein is a method of producing D-psicose. The method of producing D-psicose includes subjecting D-fructose to D-psicose epimerization to produce a D-psicose-containing solution, subjecting the D-psicose-containing solution to first cooling and ion purification, subjecting the purified D-psicose-containing solution to first concentration and second cooling, subjecting the D-psicose-containing solution, which has been subjected to first concentration and second cooling, to chromatography to obtain a D-fructose-containing mother liquor and a D-psicose-containing separated solution, and subjecting the D-psicose-containing separated solution to second concentration and third cooling to obtain D-psicose crystals, wherein the D-fructose-containing mother liquor produced by chromatography is reused in the D-psicose epimerization.

    A Genus of Epimerase Enzymes for Conversion of Fructose to Allulose at High Temperature and Low pH
    7.
    发明申请
    A Genus of Epimerase Enzymes for Conversion of Fructose to Allulose at High Temperature and Low pH 审中-公开

    公开(公告)号：US20180112244A1

    公开(公告)日：2018-04-26

    申请号：US15575636

    申请日：2016-05-20

    申请人： Archer Daniels Midland Company

    发明人： Padmesh Venkitasubramanian ,  William Schroeder ,  Mark Welch ,  Sridhar Govindarajan

    IPC分类号： C12P19/24 ,  C12P19/02 ,  C12N9/90

    CPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02

    摘要： Disclosed herein are three geneses of proteins herein established to exhibit a fructose to allulose epimerase activity that are useful for production of allulose from fructose at high temperatures and at low pH in the range of 4.5 to 6.0. Two of the three geneses descend phylogenetically from a common ancestral protein defined herein, and these geneses are distinguished from each other by different parental descendant proteins also defined herein. The proteins with high levels of sequence identity to the parental nodes defining from these two geneses generally exhibit higher levels of specific fructose to glucose epimerase activity than prior known fructose to allulose epimerases and exhibit such activity at low pH. A third genus is not defined by phylogenetic origin except by not descending from the same ancestor as the first two geneses but generally exhibit similar levels of fructose to allulose epimerase activities as prior art epimerases described to be useful for fructose to allulose conversion.