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公开(公告)号：US20210002617A1
公开(公告)日：2021-01-07
申请号：US16963964
申请日：2019-01-22
申请人： METGEN OY
IPC分类号： C12N9/02
摘要： The present invention relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the invention relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 90% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises at least one amino acid selected from the group consisting of Alanine, Proline, Aspartic acid, Isoleucine, Proline, Proline, Threonine and Proline at a position corresponding to of positions 253, 128, 384, 364, 292, 450, 33 and 322 in SEQ ID NO: 1 respectively.
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公开(公告)号：US09896667B2
公开(公告)日：2018-02-20
申请号：US15303486
申请日：2015-04-11
申请人： METGEN OY
发明人： Klara Birikh
CPC分类号： C12N9/0061 , C12Y110/03002
摘要： This application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the disclosure relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 75% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises an amino acid residue selected from the group consisting of threonine, proline, alanine, glycine, serine, cysteine, aspartic acid, asparagine and valine at a position corresponding to amino acid 149 of SEQ ID NO: 1.
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公开(公告)号：US20170121690A1
公开(公告)日：2017-05-04
申请号：US15128916
申请日：2015-03-24
申请人： METGEN OY
发明人： Klara Birikh
IPC分类号： C12N9/02
CPC分类号： C12N9/0061 , C12Y110/03002
摘要： The present application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the application relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 60% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises an alanine residue at a position corresponding to amino acid 260 of SEQ ID NO: 1.
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公开(公告)号：US20170037385A1
公开(公告)日：2017-02-09
申请号：US15304503
申请日：2015-04-16
申请人： METGEN OY
发明人： Klara Birikh
IPC分类号： C12N9/02
CPC分类号： C12N9/0061 , C12Y110/03002
摘要： This application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the disclosure relates to a polypeptide with laccase activity comprising an amino acid sequence that is more than 80% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises a non-polar amino acid, preferably an amino acid residue selected from the group consisting of proline, alanine, glycine and valine at a position corresponding to amino acid 113 of SEQ ID NO: 1.
摘要翻译： 本申请涉及漆酶变体及其在各种工业过程中作为环保生物催化剂的用途。 更具体地，本公开涉及具有漆酶活性的多肽，其包含与根据SEQ ID NO：1的氨基酸序列大于80％相同的氨基酸序列，其中所述多肽包含非极性氨基酸，优选 在对应于SEQ ID NO：1的氨基酸113的位置上选自脯氨酸，丙氨酸，甘氨酸和缬氨酸的氨基酸残基。
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公开(公告)号：US20160069024A1
公开(公告)日：2016-03-10
申请号：US14778527
申请日：2013-03-20
申请人： METGEN OY
发明人： Klara Birikh , Alexey Azhayev
CPC分类号： D21H17/005 , C12N9/0061 , C12P2201/00 , C12Y110/03002 , D21B1/02 , D21B1/021 , D21C5/005 , D21C11/0007
摘要： The present invention is in the field of paper production, more in particular it relates to the process of wood pulping. It provides useful biological methods and compounds for reducing the energy requirements of the production of wood pulp. It describes a method for reducing the energy requirement of a thermo-mechanical pulping (TMP) process wherein cellulose fibers are recovered from a biomass comprising lignocellulosic material, wherein the lignocellulosic material is treated with a CotA laccase before recovering the cellulose from the lignocellulosic material.
摘要翻译： 本发明在纸张生产领域，更特别涉及木材制浆过程。 它为减少木浆生产的能量需求提供了有用的生物学方法和化合物。 它描述了一种降低热机械制浆（TMP）方法的能量需求的方法，其中纤维素纤维从包含木质纤维素材料的生物质中回收，其中木质纤维素材料在从木质纤维素材料回收纤维素之前用CotA漆酶处理。
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公开(公告)号：US11008562B2
公开(公告)日：2021-05-18
申请号：US16062586
申请日：2016-12-08
申请人： Metgen OY
摘要： The invention is in the field of protein chemistry, in particular in the field of enzymology. It provides pectinases, i.e. polypeptides with pectin-degrading properties. In particular the invention provides polypeptides with pectate lyase activity (EC 4.2.2.2). Enzymes according to the invention have improved properties, such as improved thermostability and decreased calcium dependence.
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公开(公告)号：US10815472B2
公开(公告)日：2020-10-27
申请号：US16311611
申请日：2017-06-20
申请人： METGEN OY
摘要： The invention is in the field of biotechnology and involves recombinant DNA technology. It provides means and methods for obtaining an insoluble active fusion protein comprising xylose isomerase activity. More in particular, the invention relates to a method for obtaining active insoluble xylose isomerase, comprising the expression in a host organism of a recombinant gene encoding a fusion protein comprising a xylose isomerase in combination with a PPIase, thereby obtaining the active insoluble xylose isomerase. It also provides recombinant fusion proteins comprising xylose isomerase activity as well as their use in converting xylose to xylulose and glucose to fructose.
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公开(公告)号：US10815471B2
公开(公告)日：2020-10-27
申请号：US16062583
申请日：2016-12-08
申请人： Metgen OY
摘要： The invention relates to processes for the conversion of biomass into carbohydrates, notably reducing sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in methods wherein cellulose is converted into sugars using a cellulose-converting enzyme. More in particular, the invention provides a method for producing a reducing sugar from a lignocellulosic material, wherein the lignocellulosic material is contacted with a pectate lyase at a pH between 9 and 12, wherein the pectate lyase comprises an amino acid sequence according to SEQ ID NO: 1 or an amino acid sequence at least 70% identical with SEQ ID NO: 1.
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公开(公告)号：US20200149027A1
公开(公告)日：2020-05-14
申请号：US16062586
申请日：2016-12-08
申请人： Metgen OY
摘要： The invention is in the field of protein chemistry, in particular in the field of enzymology. It provides pectinases, i.e. polypeptides with pectin-degrading properties. In particular the invention provides polypeptides with pectate lyase activity (EC 4.2.2.2). Enzymes according to the invention have improved properties, such as improved thermostability and decreased calcium dependence.
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公开(公告)号：US20190185891A1
公开(公告)日：2019-06-20
申请号：US16311606
申请日：2017-06-20
申请人： METGEN OY
CPC分类号： C12P19/02 , C12P19/24 , C12P2201/00 , C12Y503/01005
摘要： The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of glucose into fructose wherein the glucose is derived from lignocellulosic material. More in particular, it provides a method for converting glucose into fructose comprising the steps of: providing a composition comprising water, glucose and lignin, enzymatically converting the glucose to fructose in the presence of a glucose isomerase, and optionally purifying the fructose from the solution, wherein the glucose isomerase comprises an amino acid sequence that is at least 90% identical with the sequence according to SEQ ID NO: 1 or SEQ ID NO: 2.
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