知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

41 条记录 (耗时 0.025 秒)

    PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    2.
    发明申请
    PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
    来自大麦酚酞的PECTIN降解酶及其用途

    公开(公告)号：US20150259667A1

    公开(公告)日：2015-09-17

    申请号：US14421729

    申请日：2013-08-15

    申请人： Bangladesh Jute Research Institute ,  RAFIA HASINA

    发明人： Maqsudul Alam ,  Mohammed S. Islam ,  Mohammed M. Hossen ,  Mohammed S. Haque ,  Mohammed M. Alam

    IPC分类号： C12N9/88 ,  C12N9/18

    CPC分类号： C12N9/88 ,  C12N9/18 ,  C12N9/2405 ,  C12Y301/01011 ,  C12Y402/02002 ,  C12Y402/02023

    摘要： The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

    摘要翻译： 本发明公开了分离的多核苷酸编码酶，该多核苷酸来自真菌巨噬细胞相容素（“M. phaseolina”），其负责降解果胶，并且其包含和/或由SEQ ID NO：1所示的核苷酸序列组成。 编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，1,4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61，或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌，其包含具有或具有增强的果胶降解酶生产的重组基因构建体。 本发明的多肽可以用于制造果汁，纺织品，纸浆和纸，咖啡，茶和油提取和果胶废水处理。

    METHOD OF MANUFACTURING A LYSIN PROTEIN CAPABLE OF DIRECTLY LYSING ACINETOBACTER BAUMANNII
    8.
    发明申请
    METHOD OF MANUFACTURING A LYSIN PROTEIN CAPABLE OF DIRECTLY LYSING ACINETOBACTER BAUMANNII 审中-公开
    一种能直接诱导乙酰胆碱酯酶的蛋白质蛋白质的制备方法

    公开(公告)号：US20160312203A1

    公开(公告)日：2016-10-27

    申请号：US15203638

    申请日：2016-07-06

    申请人： I-SHOU UNIVERSITY

    发明人： Chih-Hsin Hung ,  Huei-Ru Luo ,  Jen-Yi Wang

    IPC分类号： C12N9/36

    CPC分类号： C12N9/2462 ,  C07K2319/00 ,  C07K2319/74 ,  C12N9/88 ,  C12N2795/00022 ,  C12Y302/01017 ,  C12Y402/02002

    摘要： The invention discloses a method of manufacturing a lysin protein capable of directly lysing Acinetobacter baumannii without pretreatment processing using chloroform or EDTA, comprising: transforming an expression plasmid into E. coli, wherein the expression plasmid is deposited at DSMA-Deutsche Sammlung von Mikroorganismen and Zellkulturen with deposit number DSM32023; expressing the expressing plasmid by the E. coli to form lysin protein having an amino acid sequence as set forth in SEQ ID NO: 6; lysing the E. coli containing the lysing protein to obtain a supernatant by centrifugating the E. coli lysate; mixing the supernatant and Ni2+ resins, washing the unbound protein by a binding buffer containing 50 mM Tris-HCl (pH 8.2), 15 mM MgCl2, 20% (v/v) glycerol, 0.05% β-ME and 0.1 mM PMSF; and eluting the purified lysing protein by an elution buffer containing 50 mM Tris-HCl (pH 8.2), 15 mM MgCl2, 20% (v/v) glycerol, 0.05% β-ME, 0.1 mM PMSF and 250 mM imidazole.

    摘要翻译： 本发明公开了一种能够直接溶解鲍氏不动杆菌而不用氯仿或EDTA进行预处理的赖氨酸蛋白质的制造方法，其特征在于：将表达质粒转化到大肠杆菌中，其中所述表达质粒沉积在DSMA-Deutsche Sammlung von Mikroorganismen和Zellkulturen 存款编号DSM32023; 用大肠杆菌表达表达质粒以形成具有SEQ ID NO：6所示氨基酸序列的赖氨酸蛋白; 裂解含有裂解蛋白的大肠杆菌，通过离心大肠杆菌裂解液得到上清液; 混合上清液和Ni2 +树脂，通过含有50mM Tris-HCl（pH 8.2），15mM MgCl 2，20％（v / v）甘油，0.05％β-ME和0.1mM PMSF的结合缓冲液洗涤未结合的蛋白质; 并通过含有50mM Tris-HCl（pH 8.2），15mM MgCl 2，20％（v / v）甘油，0.05％β-ME，0.1mM PMSF和250mM咪唑的洗脱缓冲液洗脱纯化的裂解蛋白。