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    TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS AND PHOTOBIOREACTOR
    2.
    发明申请
    TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS AND PHOTOBIOREACTOR 失效
    转移光电微生物和光电子器件

    公开(公告)号：US20090181434A1

    公开(公告)日：2009-07-16

    申请号：US12348887

    申请日：2009-01-05

    申请人： John Aikens ,  Robert J. Turner

    发明人： John Aikens ,  Robert J. Turner

    IPC分类号： C12P19/02 ,  C12M1/00 ,  C12N1/21 ,  C12N1/13 ,  C12N15/00 ,  C12P19/00

    CPC分类号： C12M21/02 ,  C12M23/20 ,  C12M23/22 ,  C12M23/24 ,  C12M23/26 ,  C12M25/02 ,  C12N15/74 ,  C12N15/80 ,  C12P19/02 ,  C12P19/12

    摘要： Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.

    摘要翻译： 本文提供了工程化以积累碳水化合物，例如二糖的转基因细菌。 还提供了用于培养光合微生物的光生物反应器，其包含适于向光合微生物提供营养物和水分的非凝胶状固体培养载体和覆盖培养载体表面的至少一部分的物理屏障。 公开了用于大规模和连续培养包含光生物反应器的光合微生物和使用方法的装置。 还公开了使用本发明的光生物反应器从光合微生物产生可发酵糖的方法。

    SELF CONTAINED SOLID PHASE PHOTOBIOREACTOR
    4.
    发明申请
    SELF CONTAINED SOLID PHASE PHOTOBIOREACTOR 审中-公开
    自包含固体相位光电子器件

    公开(公告)号：US20120309090A1

    公开(公告)日：2012-12-06

    申请号：US13487070

    申请日：2012-06-01

    申请人： John Aikens ,  Robert J. Turner ,  Denise L. Holzle

    发明人： John Aikens ,  Robert J. Turner ,  Denise L. Holzle

    IPC分类号： C12M1/42 ,  C12M1/12 ,  C12N1/12 ,  C12N1/14 ,  C12N1/10 ,  C12N5/04 ,  C12M1/00 ,  C12N1/20

    CPC分类号： C12M21/02 ,  C12M23/22 ,  C12M25/02 ,  C12M29/00 ,  C12M33/00 ,  C12M41/06

    摘要： A compressible bioreactor for cultivating photosynthetic microorganisms, the bioreactor comprising a feeder trough, a collection trough, a growth fabric, and a barrier layer, where the bioreactor has a compressed mode and an extended mode, the growth fabric is coupled to the feeder trough and the collection trough, the growth fabric is substantially extended in the extended mode of the bioreactor, the growth fabric is substantially compressed in the compressed mode of the bioreactor and the barrier layer is coupled to the feeder base and the collection base that encases the growth fabric in a substantially airtight environment

    摘要翻译： 用于培养光合微生物的可压缩生物反应器，所述生物反应器包括进料槽，收集槽，生长织物和阻挡层，其中所述生物反应器具有压缩模式和延伸模式，所述生长织物联接到所述进料槽， 收集槽，生长织物在生物反应器的扩展模式下基本上延伸，生长织物在生物反应器的压缩模式下基本上被压缩，并且阻挡层耦合到馈送器基座和包围生长织物的收集基底 在一个基本上气密的环境中

    REGULATED GENE EXPRESSION SYSTEMS AND CONSTRUCTS THEREOF
    6.
    发明申请
    REGULATED GENE EXPRESSION SYSTEMS AND CONSTRUCTS THEREOF 有权
    调节基因表达系统及其构建

    公开(公告)号：US20120244622A1

    公开(公告)日：2012-09-27

    申请号：US13430381

    申请日：2012-03-26

    申请人： Robert J. Turner ,  Valerie Sershon ,  John Aikens ,  Denise Holzle

    发明人： Robert J. Turner ,  Valerie Sershon ,  John Aikens ,  Denise Holzle

    IPC分类号： C12N15/63 ,  C12N1/11 ,  C12N1/15 ,  C12N1/13 ,  C12N1/21

    CPC分类号： C12N15/635

    摘要： Compositions and methods for nitrogen sensitive regulation of expression of a transcribable nucleic acid molecule. One aspect provides a nitrogen-sensitive expression system that includes a transcription factor region comprising an NtcA binding site and a core promoter region comprising a RuBisCo promoter or a variant or a functional fragment thereof. Another aspect provides a method of transforming a host cell with an expression system. Also provided are expression cassettes, transformed host cells, and kits.

    摘要翻译： 可转录核酸分子表达的氮敏感调节的组成和方法。 一方面提供了一种氮敏感性表达系统，其包括包含NtcA结合位点和包含RuBisCo启动子或其变体或功能片段的核心启动子区的转录因子区域。 另一方面提供了用表达系统转化宿主细胞的方法。 还提供了表达盒，转化的宿主细胞和试剂盒。

    Regulated gene expression systems and constructs thereof
    9.
    发明授权
    Regulated gene expression systems and constructs thereof 有权
    调节基因表达系统及其构建体

    公开(公告)号：US09506072B2

    公开(公告)日：2016-11-29

    申请号：US13430381

    申请日：2012-03-26

    申请人： Robert J. Turner ,  Valerie Sershon ,  John Aikens ,  Denise Holzle

    发明人： Robert J. Turner ,  Valerie Sershon ,  John Aikens ,  Denise Holzle

    IPC分类号： C12N15/63

    CPC分类号： C12N15/635

    摘要： Compositions and methods for nitrogen sensitive regulation of expression of a transcribable nucleic acid molecule. One aspect provides a nitrogen-sensitive expression system that includes a transcription factor region comprising an NtcA binding site and a core promoter region comprising a RuBisCo promoter or a variant or a functional fragment thereof. Another aspect provides a method of transforming a host cell with an expression system. Also provided are expression cassettes, transformed host cells, and kits.

    摘要翻译： 可转录核酸分子表达的氮敏感调节的组成和方法。 一方面提供了一种氮敏感性表达系统，其包括包含NtcA结合位点和包含RuBisCo启动子或其变体或功能片段的核心启动子区的转录因子区域。 另一方面提供了用表达系统转化宿主细胞的方法。 还提供了表达盒，转化的宿主细胞和试剂盒。

    Transgenic photosynthetic microorganisms and photobioreactor
    10.
    发明授权
    Transgenic photosynthetic microorganisms and photobioreactor 失效
    转基因光合微生物和光生物反应器

    公开(公告)号：US08367379B2

    公开(公告)日：2013-02-05

    申请号：US12348887

    申请日：2009-01-05

    申请人： John Aikens ,  Robert J. Turner

    发明人： John Aikens ,  Robert J. Turner

    IPC分类号： C12P19/02

    CPC分类号： C12M21/02 ,  C12M23/20 ,  C12M23/22 ,  C12M23/24 ,  C12M23/26 ,  C12M25/02 ,  C12N15/74 ,  C12N15/80 ,  C12P19/02 ,  C12P19/12

    摘要： Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.

    摘要翻译： 本文提供了工程化以积累碳水化合物，例如二糖的转基因细菌。 还提供了用于培养光合微生物的光生物反应器，其包含适于向光合微生物提供营养物和水分的非凝胶状固体培养载体和覆盖培养载体表面的至少一部分的物理屏障。 公开了用于大规模和连续培养包含光生物反应器的光合微生物和使用方法的装置。 还公开了使用本发明的光生物反应器从光合微生物产生可发酵糖的方法。