知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

17 条记录 (耗时 0.017 秒)

    Novel Alcohol Dehydrogenases
    2.
    发明申请
    Novel Alcohol Dehydrogenases 有权
    新型酒精脱氢酶

    公开(公告)号：US20080216181A1

    公开(公告)日：2008-09-04

    申请号：US10593119

    申请日：2005-03-10

    申请人： Renate Schulze ,  Patrick Lorenz ,  Jurgen Eck ,  Oliver May ,  Harald Groger ,  Harald Trauthwein

    发明人： Renate Schulze ,  Patrick Lorenz ,  Jurgen Eck ,  Oliver May ,  Harald Groger ,  Harald Trauthwein

    IPC分类号： A01K67/027 ,  C12N9/02 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06

    CPC分类号： C12P7/02 ,  C12N9/0006 ,  C12Y101/01002

    摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to non-human hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohol's which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohol's with the formation of aldehydes or ketones.

    摘要翻译： 本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。 本发明还涉及编码所述多肽，非人宿主或宿主细胞的核酸以及可用于制备所需产物的反应体系。 本发明的多肽优选用于从醛或酮开始制备可以作为药物中间体的初级和对映体纯的仲醇。 或者，也可以将本发明的多肽用于反向反应，即醇与醛或酮的形成的氧化。

    Reca-negative and rhab-negative microorganism
    4.
    发明申请
    Reca-negative and rhab-negative microorganism 审中-公开
    Reca阴性和Rhab阴性微生物

    公开(公告)号：US20070128689A1

    公开(公告)日：2007-06-07

    申请号：US10492939

    申请日：2002-11-11

    申请人： Oliver May ,  Klaus Liebeton ,  Jurgen Eck

    发明人： Oliver May ,  Klaus Liebeton ,  Jurgen Eck

    IPC分类号： C12P21/06 ,  C12N9/16 ,  C12N15/74 ,  C07H21/04 ,  C12N1/21

    CPC分类号： C12R1/19 ,  C12N15/68 ,  C12N15/70 ,  C12P13/04 ,  C12P21/02 ,  C12P41/006

    摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-in-ducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, ex-pression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.

    摘要翻译： 本发明描述了专门用作用于鼠李糖苷可启动子的表达系统的新微生物。 由于在这些生物体中人造产生的鼠李糖和重组缺陷的组合，需要较少昂贵的鼠李糖用于诱导并因此可以更廉价地诱导并且具有较高遗传稳定性的预压系统。

    PREPARATION OF AN ESTERASE
    6.
    发明申请
    PREPARATION OF AN ESTERASE 有权
    蛋白酶的制备

    公开(公告)号：US20110177553A1

    公开(公告)日：2011-07-21

    申请号：US12667414

    申请日：2008-07-04

    申请人： Martin Kietzmann ,  Helmut Schwab ,  Harald Pichler ,  Mirela Ivancic ,  Oliver May ,  Rudolf Gijsbertus Marie Luiten

    发明人： Martin Kietzmann ,  Helmut Schwab ,  Harald Pichler ,  Mirela Ivancic ,  Oliver May ,  Rudolf Gijsbertus Marie Luiten

    IPC分类号： C12P21/02 ,  C12N1/21 ,  C12N15/55 ,  C12N15/70 ,  C12P7/40

    CPC分类号： C12N9/18 ,  C12N15/70 ,  C12Y301/01001

    摘要： The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.

    摘要翻译： 本发明涉及重组大肠杆菌菌株，其特征在于生物体含有编码具有酯酶活性的蛋白质的基因。 这种大肠杆菌适用于制备具有酯酶活性的蛋白质，从而在不与GroEL和/或GroES共同表达质粒的情况下进行表达。 谷胱甘肽还原酶和/或硫氧还蛋白还原酶的表达可以通过突变来消除。 此外，生物体在富氧条件下生长的能力已经通过突变恢复。 具有酯酶活性的蛋白质基因的表达与编码热休克分子伴侣蛋白的另外的基因的表达无关。

    Preparation of enantiomerically enriched amines and amides by enzymatic resolution using a sulfonylacetic acid ester as acyl donor
    9.
    发明授权
    Preparation of enantiomerically enriched amines and amides by enzymatic resolution using a sulfonylacetic acid ester as acyl donor 失效
    使用磺酰基乙酸酯作为酰基供体通过酶拆分制备对映体富集的胺和酰胺

    公开(公告)号：US07824899B2

    公开(公告)日：2010-11-02

    申请号：US11766164

    申请日：2007-06-21

    申请人： Harald Groeger ,  Oliver May ,  Kai Rossen ,  Karlheinz Drauz

    发明人： Harald Groeger ,  Oliver May ,  Kai Rossen ,  Karlheinz Drauz

    IPC分类号： C12P41/00

    CPC分类号： C12P11/00 ,  C12P13/001 ,  C12P13/02 ,  C12P41/007

    摘要： A process for the preparation of an enantiomerically enriched amine, is performed by a) cleaving a racemic mixture of a reaction product of i) a chiral amine and ii) an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; or b) reacting an amine with an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; and c) separating the enantiomerically enriched amide from the enantiomerically enriched amine, wherein the acyl donor is a sulphonylacetic acid ester. The process (1) leads to high enantioselectivities, and (2) high reactivities, (3) is based on an acyl donor accessible in a simple manner, (4) is suitable for a large number of very different substrates and/or (5) is suitable for carrying out at high substrate concentrations.

    摘要翻译： 通过a）在水解酶存在下，将i）手性胺和ii）酰基供体的反应产物的外消旋混合物切割，制备对映体富集的胺的方法，得到 对映异构体富集的酰胺和对映体富集的胺; 或b）在水解酶存在下使胺与酰基供体反应，得到对映体富集的酰胺和对映体富集的胺的混合物; 和c）从对映异构体富集的胺分离对映体富集的酰胺，其中所述酰基供体是磺酰基乙酸酯。 方法（1）导致高对映选择性，和（2）高反应性，（3）基于可以简单方式获得的酰基供体，（4）适用于大量非常不同的底物和/或（5 ）适用于在高底物浓度下进行。