公开(公告)号：US11999943B2

公开(公告)日：2024-06-04

申请号：US16398401

申请日：2019-04-30

申请人： INV Nylon Chemicals Americas, LLC

发明人： Alexander Brett Foster ,  Emilie Sophie Fritsch

IPC分类号： C12N1/20 ,  C12N9/02 ,  C12N9/14 ,  C12N15/52 ,  C12N15/68 ,  C12P7/16 ,  C12R1/38

CPC分类号： C12N1/205 ,  C12N9/0073 ,  C12N9/14 ,  C12N15/52 ,  C12N15/68 ,  C12P7/16 ,  C12R2001/38 ,  C12Y401/01031 ,  C12Y401/01032 ,  C12Y604/01001

摘要： Methods of redirecting carbon flux and increasing C2/C3 or a C4/5/6 carbon chain length carbon-based chemical product yield in an organism, nonnaturally occurring organisms with redirected carbon flux and increased C2/C3 or C4/5/6 carbon chain length carbon-based chemical product yield and methods for using these organisms in production of C2/C3 or C4/5/6 carbon chain length carbon-based chemical products are provided.

公开(公告)号：US20230416763A1

公开(公告)日：2023-12-28

申请号：US18037119

申请日：2021-11-12

申请人： ATELERIX LIMITED

发明人： Dean HALLAM ,  Stephen SWIOKLO ,  Che CONNON

IPC分类号： C12N15/68

CPC分类号： C12N15/68

摘要： The present invention provides a reversibly cross-linked hydrogel comprising a sample, wherein the sample comprises extracellular nucleic acid. Corresponding methods of preparing, transporting and/or storing the nucleic acid-containing hydrogel, as well as uses thereof, are also provided herein.

公开(公告)号：US20180044687A1

公开(公告)日：2018-02-15

申请号：US15534496

申请日：2015-12-11

申请人： CureVac AG

发明人： Andreas THESS ,  Thomas SCHLAKE ,  Stefanie GRUND

IPC分类号： C12N15/67 ,  C12N15/85 ,  C12N15/68 ,  A61K39/145 ,  A61K39/205

CPC分类号： C12N15/67 ,  A61K39/12 ,  A61K39/145 ,  A61K39/205 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/575 ,  C12N15/68 ,  C12N15/85 ,  C12N2760/16134 ,  C12N2760/16171 ,  C12N2760/20134 ,  C12N2760/20171 ,  C12N2830/50

摘要： The invention relates to an artificial nucleic acid molecule comprising an open reading frame and a 3′-UTR comprising at least one poly(A) sequence or a polyadenylation signal. The invention further relates to a vector comprising the artificial nucleic acid molecule comprising an open reading frame and a 3′-UTR comprising at least one poly(A) sequence or a polyadenylation signal, to a cell comprising the artificial nucleic acid molecule or the vector, to a pharmaceutical composition comprising the artificial nucleic acid molecule or the vector and to a kit comprising the artificial nucleic acid molecule, the vector and/or the pharmaceutical composition. The invention also relates to a method for increasing protein production from an artificial nucleic acid molecule and to the use of a 3′-UTR for a method for increasing protein production from an artificial nucleic acid molecule. Moreover, the invention concerns the use of the artificial nucleic acid molecule, the vector, the kit or the pharmaceutical composition as a medicament, as a vaccine or in gene therapy.

公开(公告)号：US09752154B2

公开(公告)日：2017-09-05

申请号：US14416799

申请日：2013-07-23

申请人： DOW AGROSCIENCES LLC ,  Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

发明人： Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

IPC分类号： C12N15/68 ,  C12N15/82

CPC分类号： C12N15/8209 ,  C12N15/68 ,  C12N15/82

摘要： This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

公开(公告)号：US20170166617A1

公开(公告)日：2017-06-15

申请号：US15360362

申请日：2016-11-23

申请人： Guo-Jie Ye ,  Jilin Liu

发明人： Guo-Jie Ye ,  Jilin Liu

IPC分类号： C07K14/47 ,  C12N15/86 ,  C12N7/00

CPC分类号： C07K14/4702 ,  C12N7/00 ,  C12N9/00 ,  C12N15/00 ,  C12N15/64 ,  C12N15/68 ,  C12N15/86 ,  C12N2710/16621 ,  C12N2750/14143 ,  C12N2830/008

摘要： This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications.

公开(公告)号：US20170009244A1

公开(公告)日：2017-01-12

申请号：US15217555

申请日：2016-07-22

申请人： BioNTech AG

发明人： Ugur Sahin ,  Silke Holtkamp ,  Ozlem Tureci ,  Sebastian Kreiter

IPC分类号： C12N15/68 ,  C12N15/85

CPC分类号： C12N15/68 ,  C12N15/67 ,  C12N15/85

摘要： It was the object of the present invention to provide RNA with increased stability and translation efficiency and means for obtaining such RNA. It should be possible to obtain increased grades of expression by using said RNA in gene therapy approaches.

摘要翻译： 本发明的目的是提供具有增加的稳定性和翻译效率的RNA以及获得这种RNA的手段。 通过在基因治疗方法中使用所述RNA可以获得更高等级的表达。

公开(公告)号：US20150368686A1

公开(公告)日：2015-12-24

申请号：US14605513

申请日：2015-01-26

申请人： RINA-NETZWERK RNA-TECHNOLGIEN GMBH

发明人： Helmut Merk ,  Volker Erdmann ,  Wolfgang Stiege

IPC分类号： C12P19/34 ,  C07K14/47

CPC分类号： C12P19/34 ,  C07K14/4703 ,  C12N15/10 ,  C12N15/66 ,  C12N15/68

摘要： The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.

摘要翻译： 本发明涉及通过PCR制备长核酸的方法。 该方法包括以下杂交步骤：a）核酸碱基序列在3'和5'端与衔接子引物杂交; b）来自步骤a）的产物在3'和5'端与包含延伸序列的延伸引物杂交，其中具有在核酸碱基的3'和5'末端扩增和扩增的扩增序列的核酸 然后从核酸碱基序列形成序列。 本发明还涉及本发明方法的不同应用。

公开(公告)号：US08715929B2

公开(公告)日：2014-05-06

申请号：US13380736

申请日：2010-06-25

申请人： Mohammed Benghezal ,  Yakhya Dieye ,  Carola Schwann ,  Miriam Sehnal ,  Alma Fulurija ,  Barry J. Marshall

发明人： Mohammed Benghezal ,  Yakhya Dieye ,  Carola Schwann ,  Miriam Sehnal ,  Alma Fulurija ,  Barry J. Marshall

IPC分类号： C12Q1/68 ,  C12P21/06

CPC分类号： C12N15/68 ,  C12N9/80 ,  C12N15/52 ,  C12N15/74 ,  C12Y305/01005

摘要： The present invention relates to synthetic operons. In particular, the present invention relates to a synthetic operon for integration into a bacterial chromosome of a bacterium comprising a promoter operably-linked to at least two genes, wherein at least one gene is a gene of interest and at least one gene is a gene essential to said bacterium.

摘要翻译： 本发明涉及合成操纵子。 特别地，本发明涉及用于整合到细菌细菌染色体中的合成操纵子，其包含与至少两个基因可操作地连接的启动子，其中至少一个基因是目的基因，并且至少一个基因是基因 对所述细菌至关重要。

公开(公告)号：US20130115605A1

公开(公告)日：2013-05-09

申请号：US13630643

申请日：2012-09-28

申请人： Rina-Netzwerk RNA-Technolgien GMBH

发明人： Helmut Merk ,  Volker Erdmann ,  Wolfgang Stiege

IPC分类号： C12P19/34

CPC分类号： C12P19/34 ,  C07K14/4703 ,  C12N15/10 ,  C12N15/66 ,  C12N15/68

摘要： The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.

摘要翻译： 本发明涉及通过PCR制备长核酸的方法。 该方法包括以下杂交步骤：a）核酸碱基序列在3'和5'端与衔接子引物杂交; b）来自步骤a）的产物在3'和5'端与包含延伸序列的延伸引物杂交，其中具有在核酸碱基的3'和5'末端扩增和扩增的扩增序列的核酸 然后从核酸碱基序列形成序列。 本发明还涉及本发明方法的不同应用。

公开(公告)号：US20090093433A1

公开(公告)日：2009-04-09

申请号：US12185012

申请日：2008-08-01

申请人： Tod Woolf ,  Kristin Wiederholt ,  Margaret Taylor

发明人： Tod Woolf ,  Kristin Wiederholt ,  Margaret Taylor

IPC分类号： A61K31/7105 ,  C07H21/02 ,  C12P19/34

CPC分类号： C12N15/68 ,  C12N15/67

摘要： The present invention describes novel methods for the stabilization of mRNA. These alterations increase stability of mRNA and enable its use in sense RNA therapy to transiantly express proteins in a cell. Accordingly, the present invention is directed to methods for making such modifications, compositions comprising such modifications, and the use of such compositions in treating disease states.

摘要翻译： 本发明描述了稳定mRNA的新方法。 这些改变增加了mRNA的稳定性，并使其能够在有意义的RNA治疗中将细胞中的蛋白质转染表达。 因此，本发明涉及制备这种修饰的方法，包含这些修饰的组合物以及这些组合物在治疗疾病状态中的用途。