-
公开(公告)号：US20170327859A1
公开(公告)日：2017-11-16
申请号：US15388164
申请日：2016-12-22
发明人： Helmut Merk , Volker Erdmann , Wolfgang Stiege
CPC分类号： C12P19/34 , C07K14/4703 , C12N15/10 , C12N15/66 , C12N15/68
摘要： The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.
-
公开(公告)号：US20150368686A1
公开(公告)日：2015-12-24
申请号：US14605513
申请日：2015-01-26
发明人： Helmut Merk , Volker Erdmann , Wolfgang Stiege
CPC分类号： C12P19/34 , C07K14/4703 , C12N15/10 , C12N15/66 , C12N15/68
摘要： The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.
摘要翻译： 本发明涉及通过PCR制备长核酸的方法。 该方法包括以下杂交步骤：a）核酸碱基序列在3'和5'端与衔接子引物杂交; b）来自步骤a）的产物在3'和5'端与包含延伸序列的延伸引物杂交，其中具有在核酸碱基的3'和5'末端扩增和扩增的扩增序列的核酸 然后从核酸碱基序列形成序列。 本发明还涉及本发明方法的不同应用。
-
公开(公告)号：US20130115605A1
公开(公告)日：2013-05-09
申请号：US13630643
申请日：2012-09-28
发明人： Helmut Merk , Volker Erdmann , Wolfgang Stiege
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C07K14/4703 , C12N15/10 , C12N15/66 , C12N15/68
摘要： The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.
摘要翻译： 本发明涉及通过PCR制备长核酸的方法。 该方法包括以下杂交步骤：a）核酸碱基序列在3'和5'端与衔接子引物杂交; b）来自步骤a）的产物在3'和5'端与包含延伸序列的延伸引物杂交，其中具有在核酸碱基的3'和5'末端扩增和扩增的扩增序列的核酸 然后从核酸碱基序列形成序列。 本发明还涉及本发明方法的不同应用。
-
-
搜索结果
3 条记录 (耗时 0.019 秒)
