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1.发明申请TREHALOSE PHOSPHORYLASES AND THEIR USE IN THE BIOCATALYTIC PRODUCTION OF TREHALOSE-ANALOGUES AND GLYCOSYL PHOSPHATES 有权TREHALOSE磷酸钙及其在TREHALOSE-ANALOGUES和GLYCOSYL PHOSPHATE生物生产中的应用公开(公告)号：US20130302857A1
公开(公告)日：2013-11-14
申请号：US13885789
申请日：2011-12-09
申请人： Jef Van Der Borght , Tom Desmet , Wim Soetaert , Chao Chen
发明人： Jef Van Der Borght , Tom Desmet , Wim Soetaert , Chao Chen
IPC分类号： C12N9/10
CPC分类号： C12N9/1051 , C12P19/02 , C12P19/12 , C12P19/18 , C12Y204/01064
摘要： The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogues and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.
摘要翻译： 本发明涉及可用于工业生产海藻糖类似物和糖基磷酸盐的海藻糖磷酸酶。 更具体地，本发明公开了在特定氨基酸区域突变的海藻糖磷酸酶。 这些特异性突变导致酶修饰的底物特异性。 此外，本发明公开了一种来自海洋生物体Caldanaerobacter subterraneus的野生型海藻糖磷酸化酶及其突变型，其具有高度热稳定性并具有广泛的受体和供体特异性。
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2.发明授权Trehalose phosphorylases and their use in the biocatalytic production of trehalose-analogues and glycosyl phosphates 有权海藻糖磷酸酶及其在海藻糖类似物和糖基磷酸酯的生物催化生产中的应用公开(公告)号：US09102922B2
公开(公告)日：2015-08-11
申请号：US13885789
申请日：2011-12-09
申请人： Jef Van Der Borght , Tom Desmet , Wim Soetaert , Chao Chen
发明人： Jef Van Der Borght , Tom Desmet , Wim Soetaert , Chao Chen
CPC分类号： C12N9/1051 , C12P19/02 , C12P19/12 , C12P19/18 , C12Y204/01064
摘要： The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogs and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.
摘要翻译： 本发明涉及可用于工业生产海藻糖类似物和糖基磷酸盐的海藻糖磷酸酶。 更具体地，本发明公开了在特定氨基酸区域突变的海藻糖磷酸酶。 这些特异性突变导致酶修饰的底物特异性。 此外，本发明公开了一种来自海洋生物体Caldanaerobacter subterraneus的野生型海藻糖磷酸化酶及其突变型，其具有高度热稳定性并具有广泛的受体和供体特异性。
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公开(公告)号：US08889379B2
公开(公告)日：2014-11-18
申请号：US13696782
申请日：2011-05-19
申请人： Giang Hai Tran , Tom Desmet , Wim Soetaert
发明人： Giang Hai Tran , Tom Desmet , Wim Soetaert
CPC分类号： C12N9/1051 , C12P19/18 , C12P19/44 , C12Y204/01049
摘要： An in vitro method to produce a glycoside is described which includes the steps of contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate or alpha-galactose-1-phosphate and an acceptor, and glycosylating the acceptor. The acceptor may be an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid. Alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids are produced when alpha-glucose-1-phosphate is used as donor. Corresponding lactosides are produced when alpha-galactose-1-phosphate is used as donor.
摘要翻译： 描述了产生糖苷的体外方法，其包括使来自灭菌梭菌的纤维糊酶磷酸化酶与α-葡萄糖-1-磷酸或α-半乳糖-1-磷酸和受体接触并使受体糖基化的步骤。 受体可以是β-葡萄糖苷基，芳基β-葡糖苷，葡萄糖脂，烷基β-槐糖苷，芳基β-槐糖苷或槐糖脂。 当使用α-葡萄糖-1-磷酸作为供体时，产生烷基纤维二糖苷，芳基纤维二糖苷，纤维二糖，纤维三糖，葡萄糖脂和纤维二糖脂。 当α-半乳糖-1-磷酸被用作供体时，产生相应的乳糖苷。
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公开(公告)号：US20130029384A1
公开(公告)日：2013-01-31
申请号：US13639321
申请日：2011-04-01
申请人： An Cerdobbel , Tom Desmet , Wim Soetaert
发明人： An Cerdobbel , Tom Desmet , Wim Soetaert
CPC分类号： C12N9/1051 , C12N9/96 , C12N11/00 , C12N11/08
摘要： The present invention relates to a sucrose phosphorylase from Bifidobacterium adolescentis which is useful as a biocatalyst in carbohydrate conversions at high temperatures. Indeed, the biocatalysts of the present invention are enzymatically active for a time period of at least 16 h and up to 1 to 2 week(s) at a temperature of at least 60° C. The biocatalysts of the present invention are: a) immobilized on an enzyme carrier, or b) are part of a cross-linked enzyme aggregate (CLEA), and/or c) are mutated, and/or d) are enzymatically active in the continuous presence of their substrate.
摘要翻译： 本发明涉及一种可用作高温碳水化合物转化中的生物催化剂的青春双歧杆菌的蔗糖磷酸化酶。 实际上，本发明的生物催化剂在至少60℃的温度下具有酶活性至少16小时和至多1至2周的时间。本发明的生物催化剂是：a） 固定在酶载体上，或b）是其底物的连续存在下的交联酶聚集体（CLEA）的一部分和/或c）被突变，和/或d）具有酶活性。
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公开(公告)号：US20130059340A1
公开(公告)日：2013-03-07
申请号：US13696782
申请日：2011-05-19
申请人： Giang Hai Tran , Tom Desmet , Wim Soetaert
发明人： Giang Hai Tran , Tom Desmet , Wim Soetaert
IPC分类号： C12P19/44
CPC分类号： C12N9/1051 , C12P19/18 , C12P19/44 , C12Y204/01049
摘要： The present invention relates to the biocatalytic production of glycosides. In particular, the invention discloses a method to produce alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids via the usage of a cellodextrin phosphorylase (CDP) derived from Clostridium stercorarium (CsCDP). The method of the present invention is feasible for use in industrial glycosylation processes and has advantageous properties compared to classical as chemical glycosylation reactions. Moreover, said method can also be employed to produce corresponding lactosides such a lactolipids when a-galactose-1-phosphate instead of a-glucose-1-phosphate is used as a donor.
摘要翻译： 本发明涉及糖苷的生物催化生产。 特别地，本发明公开了一种通过使用源自灭菌梭菌（CsCDP）的纤维糊酶磷酸化酶（CDP）来生产烷基纤维二糖苷，芳基纤维二糖苷，纤维二糖，纤维三糖，葡萄糖脂和纤维二糖体脂的方法。 本发明的方法可用于工业糖基化方法，并且与经典的化学糖基化反应相比具有有利的性质。 此外，当α-半乳糖-1-磷酸酯代替α-葡萄糖-1-磷酸作为供体时，所述方法也可用于产生相应的乳糖苷如乳糖。
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公开(公告)号：US08278073B2
公开(公告)日：2012-10-02
申请号：US12735023
申请日：2008-12-19
申请人： Tom Desmet , Manu De Groeve , Wim Soetaert
发明人： Tom Desmet , Manu De Groeve , Wim Soetaert
IPC分类号： C12N9/10
CPC分类号： C12N9/1051 , C12P19/02
摘要： The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.
摘要翻译： 本发明涉及新型乳糖磷酸酶及其用途。 更具体地说，本发明涉及通过来自纤维单胞菌（Ulu）的纤维二糖磷酸化酶的突变产生的乳糖磷酸化酶。 通过在该酶中引入突变，可以将活性从纤维二糖磷酸化酶切换成乳糖磷酸化酶。
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公开(公告)号：US20110008849A1
公开(公告)日：2011-01-13
申请号：US12735023
申请日：2008-12-19
申请人： Tom Desmet , Manu De Groeve , Wim Soetaert
发明人： Tom Desmet , Manu De Groeve , Wim Soetaert
CPC分类号： C12N9/1051 , C12P19/02
摘要： The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.
摘要翻译： 本发明涉及新型乳糖磷酸酶及其用途。 更具体地说，本发明涉及通过来自纤维单胞菌（Ulu）的纤维二糖磷酸化酶的突变产生的乳糖磷酸化酶。 通过在该酶中引入突变，可以将活性从纤维二糖磷酸化酶切换成乳糖磷酸化酶。
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8.发明授权公开(公告)号：US09005923B2
公开(公告)日：2015-04-14
申请号：US13701784
申请日：2011-06-10
CPC分类号： C12N15/815 , C12N9/0071 , C12P7/64 , C12P7/6409 , C12P19/44
摘要： The present invention relates to yeast species which are normally capable of producing sophorolipids but which are modified in such way that they are incapable producing the latter compounds. These sophorolipid-negative strains surprisingly display equal growth characteristics and biomass formation as their wild type counterparts and are hence useful for the production of compounds such as recombinant proteins, glycolipids, polyhydroxyalkanoates and carotenoides. In addition, the present invention discloses two glucosyltransferase genes with key-functions in sophorolipid production.
摘要翻译： 本发明涉及通常能够生产槐糖脂但是以这样的方式进行修饰的酵母菌种，使得它们不能产生后者化合物。 这些槐油脂阴性菌株令人惊奇地显示出与其野生型对应物相同的生长特征和生物质形成，因此可用于生产化合物如重组蛋白，糖脂，聚羟基链烷酸酯和胡萝卜素。 此外，本发明公开了在槐糖脂生产中具有关键功能的两种葡糖基转移酶基因。
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9.发明申请公开(公告)号：US20130089892A1
公开(公告)日：2013-04-11
申请号：US13701784
申请日：2011-06-10
IPC分类号： C12N15/81
CPC分类号： C12N15/815 , C12N9/0071 , C12P7/64 , C12P7/6409 , C12P19/44
摘要： The present invention relates to yeast species which are normally capable of producing sophorolipids but which are modified in such way that they are incapable producing the latter compounds. These sophorolipid-negative strains surprisingly display equal growth characteristics and biomass formation as their wild type counterparts and are hence useful for the production of compounds such as recombinant proteins, glycolipids, polyhydroxyalkanoates and carotenoides. In addition, the present invention discloses two glucosyltransferase genes with key-functions in sophorolipid production.
摘要翻译： 本发明涉及通常能够生产槐糖脂但是以这样的方式进行修饰的酵母菌种，使得它们不能产生后者化合物。 这些槐油脂阴性菌株令人惊奇地显示出与其野生型对应物相同的生长特征和生物质形成，因此可用于生产化合物如重组蛋白，糖脂，聚羟基链烷酸酯和胡萝卜素。 此外，本发明公开了在槐糖脂生产中具有关键功能的两种葡糖基转移酶基因。
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公开(公告)号：US20120311741A1
公开(公告)日：2012-12-06
申请号：US13514908
申请日：2010-12-09
申请人： Wim Soetaert , Inge Van Bogaert
发明人： Wim Soetaert , Inge Van Bogaert
摘要： The invention relates to a transporter protein involved in the transport of sophorolipids. More specifically, it relates to a Candida bombicola sophorolipid transporter protein, and the use of this transporter to modulate the secretion and/or production of glycolipids, preferably sophorolipids in organisms, preferably in fungi.
摘要翻译： 本发明涉及涉及槐糖脂运输的转运蛋白。 更具体地说，本发明涉及一种假丝酵母蚕豆转运蛋白，以及该转运蛋白在生物体中，优选在真菌中调节糖脂分泌和/或产生的优点，优选为槐糖脂。
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