公开(公告)号：US08278073B2

公开(公告)日：2012-10-02

申请号：US12735023

申请日：2008-12-19

申请人： Tom Desmet ,  Manu De Groeve ,  Wim Soetaert

发明人： Tom Desmet ,  Manu De Groeve ,  Wim Soetaert

IPC分类号： C12N9/10

CPC分类号： C12N9/1051 ,  C12P19/02

摘要： The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.

摘要翻译： 本发明涉及新型乳糖磷酸酶及其用途。 更具体地说，本发明涉及通过来自纤维单胞菌（Ulu）的纤维二糖磷酸化酶的突变产生的乳糖磷酸化酶。 通过在该酶中引入突变，可以将活性从纤维二糖磷酸化酶切换成乳糖磷酸化酶。

公开(公告)号：US20110008849A1

公开(公告)日：2011-01-13

申请号：US12735023

申请日：2008-12-19

申请人： Tom Desmet ,  Manu De Groeve ,  Wim Soetaert

发明人： Tom Desmet ,  Manu De Groeve ,  Wim Soetaert

IPC分类号： C12N9/10 ,  C07H21/00 ,  C12P19/02 ,  C12P19/12

CPC分类号： C12N9/1051 ,  C12P19/02

摘要： The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.

摘要翻译： 本发明涉及新型乳糖磷酸酶及其用途。 更具体地说，本发明涉及通过来自纤维单胞菌（Ulu）的纤维二糖磷酸化酶的突变产生的乳糖磷酸化酶。 通过在该酶中引入突变，可以将活性从纤维二糖磷酸化酶切换成乳糖磷酸化酶。

公开(公告)号：US20130302857A1

公开(公告)日：2013-11-14

申请号：US13885789

申请日：2011-12-09

申请人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

发明人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

IPC分类号： C12N9/10

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/12 ,  C12P19/18 ,  C12Y204/01064

摘要： The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogues and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.

摘要翻译： 本发明涉及可用于工业生产海藻糖类似物和糖基磷酸盐的海藻糖磷酸酶。 更具体地，本发明公开了在特定氨基酸区域突变的海藻糖磷酸酶。 这些特异性突变导致酶修饰的底物特异性。 此外，本发明公开了一种来自海洋生物体Caldanaerobacter subterraneus的野生型海藻糖磷酸化酶及其突变型，其具有高度热稳定性并具有广泛的受体和供体特异性。

公开(公告)号：US09102922B2

公开(公告)日：2015-08-11

申请号：US13885789

申请日：2011-12-09

申请人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

发明人： Jef Van Der Borght ,  Tom Desmet ,  Wim Soetaert ,  Chao Chen

IPC分类号： C12N9/10 ,  C12P19/12 ,  C12P19/18 ,  C12P19/02

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/12 ,  C12P19/18 ,  C12Y204/01064

摘要： The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogs and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.

摘要翻译： 本发明涉及可用于工业生产海藻糖类似物和糖基磷酸盐的海藻糖磷酸酶。 更具体地，本发明公开了在特定氨基酸区域突变的海藻糖磷酸酶。 这些特异性突变导致酶修饰的底物特异性。 此外，本发明公开了一种来自海洋生物体Caldanaerobacter subterraneus的野生型海藻糖磷酸化酶及其突变型，其具有高度热稳定性并具有广泛的受体和供体特异性。

公开(公告)号：US08889379B2

公开(公告)日：2014-11-18

申请号：US13696782

申请日：2011-05-19

申请人： Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

发明人： Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

IPC分类号： C12P19/44 ,  C12N9/10 ,  C12P19/18

CPC分类号： C12N9/1051 ,  C12P19/18 ,  C12P19/44 ,  C12Y204/01049

摘要： An in vitro method to produce a glycoside is described which includes the steps of contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate or alpha-galactose-1-phosphate and an acceptor, and glycosylating the acceptor. The acceptor may be an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid. Alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids are produced when alpha-glucose-1-phosphate is used as donor. Corresponding lactosides are produced when alpha-galactose-1-phosphate is used as donor.

摘要翻译： 描述了产生糖苷的体外方法，其包括使来自灭菌梭菌的纤维糊酶磷酸化酶与α-葡萄糖-1-磷酸或α-半乳糖-1-磷酸和受体接触并使受体糖基化的步骤。 受体可以是β-葡萄糖苷基，芳基β-葡糖苷，葡萄糖脂，烷基β-槐糖苷，芳基β-槐糖苷或槐糖脂。 当使用α-葡萄糖-1-磷酸作为供体时，产生烷基纤维二糖苷，芳基纤维二糖苷，纤维二糖，纤维三糖，葡萄糖脂和纤维二糖脂。 当α-半乳糖-1-磷酸被用作供体时，产生相应的乳糖苷。

公开(公告)号：US20130029384A1

公开(公告)日：2013-01-31

申请号：US13639321

申请日：2011-04-01

申请人： An Cerdobbel ,  Tom Desmet ,  Wim Soetaert

发明人： An Cerdobbel ,  Tom Desmet ,  Wim Soetaert

IPC分类号： C12N9/10 ,  C12N15/70 ,  C12N11/10 ,  C12P19/02 ,  C12N11/16 ,  C12N11/08

CPC分类号： C12N9/1051 ,  C12N9/96 ,  C12N11/00 ,  C12N11/08

摘要： The present invention relates to a sucrose phosphorylase from Bifidobacterium adolescentis which is useful as a biocatalyst in carbohydrate conversions at high temperatures. Indeed, the biocatalysts of the present invention are enzymatically active for a time period of at least 16 h and up to 1 to 2 week(s) at a temperature of at least 60° C. The biocatalysts of the present invention are: a) immobilized on an enzyme carrier, or b) are part of a cross-linked enzyme aggregate (CLEA), and/or c) are mutated, and/or d) are enzymatically active in the continuous presence of their substrate.

摘要翻译： 本发明涉及一种可用作高温碳水化合物转化中的生物催化剂的青春双歧杆菌的蔗糖磷酸化酶。 实际上，本发明的生物催化剂在至少60℃的温度下具有酶活性至少16小时和至多1至2周的时间。本发明的生物催化剂是：a） 固定在酶载体上，或b）是其底物的连续存在下的交联酶聚集体（CLEA）的一部分和/或c）被突变，和/或d）具有酶活性。

公开(公告)号：US20130059340A1

公开(公告)日：2013-03-07

申请号：US13696782

申请日：2011-05-19

申请人： Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

发明人： Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

IPC分类号： C12P19/44

CPC分类号： C12N9/1051 ,  C12P19/18 ,  C12P19/44 ,  C12Y204/01049

摘要： The present invention relates to the biocatalytic production of glycosides. In particular, the invention discloses a method to produce alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids via the usage of a cellodextrin phosphorylase (CDP) derived from Clostridium stercorarium (CsCDP). The method of the present invention is feasible for use in industrial glycosylation processes and has advantageous properties compared to classical as chemical glycosylation reactions. Moreover, said method can also be employed to produce corresponding lactosides such a lactolipids when a-galactose-1-phosphate instead of a-glucose-1-phosphate is used as a donor.

摘要翻译： 本发明涉及糖苷的生物催化生产。 特别地，本发明公开了一种通过使用源自灭菌梭菌（CsCDP）的纤维糊酶磷酸化酶（CDP）来生产烷基纤维二糖苷，芳基纤维二糖苷，纤维二糖，纤维三糖，葡萄糖脂和纤维二糖体脂的方法。 本发明的方法可用于工业糖基化方法，并且与经典的化学糖基化反应相比具有有利的性质。 此外，当α-半乳糖-1-磷酸酯代替α-葡萄糖-1-磷酸作为供体时，所述方法也可用于产生相应的乳糖苷如乳糖。