公开(公告)号：US20240041939A1

公开(公告)日：2024-02-08

申请号：US18067204

申请日：2022-12-16

申请人： William Marsh Rice University

发明人： Omid VEISEH ,  Amanda NASH ,  Samira AGHLARA-FOTOVAT

IPC分类号： A61K35/36 ,  C12N5/071 ,  C07K14/54 ,  C12N11/08 ,  A61P35/00

CPC分类号： A61K35/36 ,  C12N5/0602 ,  C07K14/5434 ,  C12N11/08 ,  A61P35/00

摘要： The present disclosure relates to implantable constructs (encapsulated cells) designed to deliver therapeutic reagents, such as IL-12 and/or IL-12 and methods of using the same to treat conditions, such as cancer.

公开(公告)号：US20240026333A1

公开(公告)日：2024-01-25

申请号：US18036678

申请日：2021-11-15

申请人： The Regents of the University of Colorado, a body

发明人： JAMES S. WELTZ ,  JOEL L. KAAR ,  DANIEL K. SCHWARTZ ,  HECTOR SANCHEZ-MORAN

IPC分类号： C12N11/08 ,  C12N9/20 ,  B01J13/14

CPC分类号： C12N11/08 ,  C12N9/20 ,  B01J13/14

摘要： A polymer brush composed of statistical copolymers of hydrophilic and hydrophobic monomers for enzyme immobilization. The heterogeneous polymer brushes stabilized four different lipases against high temperatures. Notably, the statistical copolymers stabilized the four lipases to a greater extent than a homopolymer brush. Additionally, in the case of Rhizomucor miehei lipase, statistical copolymers stabilized the enzyme to a greater extent than homopolymers of either hydrophilic or hydrophobic monomers. The resulting increase in high-temperature stability enabled significant improvements in catalytic rates by operating reactions at elevated temperatures, which is desirable for enzyme catalysis and sensing applications. Additionally, stabilization against elevated temperatures implies stabilization against non-aqueous solvents, which is of critical importance to numerous applications of biocatalysts.

公开(公告)号：US11719606B2

公开(公告)日：2023-08-08

申请号：US16628595

申请日：2018-07-03

申请人： University Public Corporation Osaka

发明人： Chie Kojima ,  Akikazu Matsumoto

IPC分类号： G01N1/30 ,  C12N11/04 ,  C12N11/08 ,  G01N33/48

CPC分类号： G01N1/30 ,  C12N11/04 ,  C12N11/08 ,  G01N33/48

摘要： The present invention provides a method for rapidly clearing a biological tissue. According to the present invention, provided is a method for clearing a biological tissue, including the steps of: infiltrating the biological tissue with water-soluble ethylenically unsaturated monomers before, during or after fixing the biological tissue with a fixative, wherein the water-soluble ethylenically unsaturated monomers include at least a water-soluble ethylenically unsaturated monomer having an ionically dissociable group; polymerizing the water-soluble ethylenically unsaturated monomers to form a hydrogel in the fixed biological tissue; and removing a lipid from the fixed biological tissue.

公开(公告)号：US20230174965A1

公开(公告)日：2023-06-08

申请号：US17919114

申请日：2021-04-16

申请人： THE UNIVERSITY OF BIRMINGHAM

发明人： Francisco FERNANDEZ-TRILLO ,  Timothy William OVERTON ,  Pavan Rao ADONI ,  Omar Nicholas Edward HUNEIDI

IPC分类号： C12N11/08 ,  C12N1/20

CPC分类号： C12N11/08 ,  C12N1/20 ,  C08F126/00

摘要： A method of producing a biofilm-like microorganism-polymer complex, the method comprising the step of exposing, in an aqueous medium, cells of a microorganism to a polymer comprising groups as defined by formula (la) or formula (lb), wherein: Y comprises an imine; and R2 comprises a CI-30 group. A biofilm-like microorganism-polymer complex comprising cells of a microorganism and a polymer comprising groups as defined by formula (la) and/or formula (lb). A method for producing a chemical and/or biological product using the microorganism-polymer complex, and the chemical and/or biological products obtained therefrom. A method of dispersing the microorganism-polymer complex. Polymers of formula (la) and of formula (lb).

公开(公告)号：US20230139158A1

公开(公告)日：2023-05-04

申请号：US18089351

申请日：2022-12-27

申请人： DexCom, Inc.

发明人： Jiong Zou ,  Robert J. Boock ,  Andrew Trinin Dennis ,  Ted Tang Lee ,  Jeff T. Suri ,  David Sze ,  Mark A. Tapsak ,  Huashi Zhang ,  Shanger Wang

IPC分类号： G01N27/327 ,  C12N11/089 ,  C12N11/093 ,  C12N11/08 ,  C08L39/06 ,  C08L69/00 ,  C08L75/04 ,  C12Q1/00 ,  A61B5/00 ,  A61B5/145 ,  A61B5/1486 ,  G01N33/66 ,  A61K31/045 ,  A61K31/137 ,  A61K31/46 ,  A61K31/465 ,  A61K31/485 ,  A61K31/515 ,  A61K31/56 ,  A61K33/00 ,  A61K36/185 ,  A61K38/28 ,  A61B5/1473

摘要： Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

公开(公告)号：US11332730B2

公开(公告)日：2022-05-17

申请号：US16648919

申请日：2018-09-28

申请人： Enzymaster (Ningbo) Bio-Engineering Co., Ltd.

发明人： Haibin Chen ,  Yong Koy Bong ,  Wenhui Zheng ,  Zhaoqi Zhang ,  Kuifang He ,  Chengxiao Zhang ,  Lei Sun ,  Yingxin Zhang

IPC分类号： C12N9/88 ,  C12N11/00 ,  C12P13/06 ,  C12N11/08

摘要： The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce β-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing β-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or β-alanine. The use of the engineered polypeptides of the present invention for the preparation of β-alanine results in higher unit activity, lower cost, and has good industrial application prospects.

公开(公告)号：US11045440B2

公开(公告)日：2021-06-29

申请号：US16287543

申请日：2019-02-27

申请人： Alcresta Therapeutics, Inc.

发明人： Robert Gallotto ,  Greta L. Loring ,  Kenneth Gary ,  Edward S. Park ,  David J. Brown ,  Willem Robert Klaas Schoevaart ,  Michiel Christian Alexander van Vliet

IPC分类号： A61K31/202 ,  C12N11/02 ,  A61L29/04 ,  A61M5/14 ,  A61M5/142 ,  C12N11/06 ,  C12N11/08 ,  A61J15/00 ,  A23L33/00 ,  A23L33/12 ,  C12N9/20

摘要： Embodiments of the disclosure are drawn to an enteral feeding device for hydrolyzing triglycerides in a nutritional formula. The device may include a body housing a chamber, an inlet configured to fluidly couple with a source of nutritional formula, and an outlet configured to fluidly couple with an enteral feeding tube. The device may include a headspace and a plurality of particles contained within the chamber, wherein the lipase is covalently bonded to the plurality of particles. The device may include an inlet filter located between the inlet and the chamber, wherein the inlet filter contains a first plurality of openings, and an outlet filter located between the chamber and the outlet, wherein the outlet filter has a second plurality of openings smaller than the plurality of particles.

公开(公告)号：US10981112B2

公开(公告)日：2021-04-20

申请号：US15498279

申请日：2017-04-26

申请人： Bonno Koers

发明人： Bonno Koers

IPC分类号： B01D53/85 ,  C02F3/04 ,  B01D46/52 ,  C02F3/10 ,  C12N11/08 ,  C02F101/32 ,  C02F3/06 ,  C02F3/34 ,  C02F101/10

摘要： The present disclosure relates to a filter material which can be used in reducing the content of contaminants in a raw gas or liquid and a device which comprises the filter material. The disclosure also relates to a method for reducing the content of contaminants in a raw gas or liquid which applies the filter material and/or device.

公开(公告)号：US10927391B2

公开(公告)日：2021-02-23

申请号：US16070661

申请日：2017-12-19

申请人： Enzymocore Ltd.

发明人： Sobhi Basheer ,  Ahmad Egbarieh ,  Ramez Masri

IPC分类号： C12P7/64 ,  C12N9/20 ,  C12N11/08

摘要： Disclosed are various enzymatic processes for the enrichments of oils with omega-3 fatty acids, and specific lipase preparations for use with these processes.

公开(公告)号：US20200369844A1

公开(公告)日：2020-11-26

申请号：US16989041

申请日：2020-08-10

申请人： Bioright Worldwide Company Limited

发明人： Chung Hong Cheung ,  Jun Wang

IPC分类号： C08J9/00 ,  C07K17/06 ,  C07K17/08 ,  C07K1/22 ,  C12N11/06 ,  C12N11/08 ,  B01D15/38 ,  C12N11/02 ,  C07K17/04 ,  C12N9/10 ,  B01J20/32 ,  B01J20/286 ,  B01J20/26 ,  B01J20/24 ,  A01N43/68 ,  B01J31/02 ,  B01J31/06 ,  C07K5/083 ,  C07K7/62 ,  C07K14/31 ,  C12N11/10

摘要： The invention is directed to a complex, including: a porous composite carrier including: a porous organic foam material containing open pores, each pore comprising a wall defining the pore; and a crosslinked product having aldehyde groups and immobilized on the surface of the walls of one or more pores of the porous organic foam materials, and a protein, polypeptide, or oligopeptide immobilized onto the porous composite carrier through a reaction between an amino group of the protein, polypeptide or oligopeptide and an aldehyde group of the composite carrier. The immobilized product has high specific surface area and high specific activity. The immobilization is simple and in low cost, and is suitable for industrial application.