公开(公告)号：US20240254527A1

公开(公告)日：2024-08-01

申请号：US18260333

申请日：2021-07-28

申请人： TIANJIN YEAHE BIOTECHNOLOGY CO., LTD

发明人： Yanhe MA ,  Ting SHI ,  Yunjie LI ,  Pingping HAN ,  Yuan LI

IPC分类号： C12P19/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/90 ,  C12N11/00 ,  C12N15/75

CPC分类号： C12P19/02 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/90 ,  C12N11/00 ,  C12N15/75 ,  C12Y204/01001 ,  C12Y301/03 ,  C12Y501/03 ,  C12Y503/01009 ,  C12Y504/02002

摘要： Provided are a Bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.

公开(公告)号：US20240150690A1

公开(公告)日：2024-05-09

申请号：US17981714

申请日：2022-11-07

申请人： Ralph L. Stirling

发明人： Ralph L. Stirling

IPC分类号： C12M3/00 ,  B33Y30/00 ,  C12M1/12 ,  C12M1/26 ,  C12N11/00

CPC分类号： C12M21/08 ,  B33Y30/00 ,  C12M25/14 ,  C12M33/04 ,  C12N11/00

摘要： A method for producing tissue constructs formed of one or more materials, dispensed or applied in patterned layers on two or more platforms, build plates or print surfaces by two or more stations, performing dispensing, mechanical modification, chemical modification, biological modification, or inspection operations. The platforms are positioned relative to the stations and station operations are directed by signals from a controller causing station operations on platforms to be performed simultaneously, for each patterned layer until all layers have been applied in sequence.

公开(公告)号：US11959105B2

公开(公告)日：2024-04-16

申请号：US16914195

申请日：2020-06-26

申请人： Quantum-Si Incorporated

发明人： Brian Reed ,  Manjula Pandey

IPC分类号： C12N9/12 ,  C12N1/21 ,  C12N11/00 ,  C12P19/34 ,  C12Q1/6806

CPC分类号： C12N9/1247 ,  C12N11/00 ,  C12P19/34 ,  C12Q1/6806 ,  C12Y207/07006 ,  C07K2319/21

摘要： Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US11866693B2

公开(公告)日：2024-01-09

申请号：US17061617

申请日：2020-10-02

申请人： HYUNDAI MOTOR COMPANY ,  KIA MOTORS CORPORATION

发明人： Kwangmo Seong ,  Heungsik Kim ,  Mi Jung Yun

IPC分类号： C12N11/00 ,  A61L2/232 ,  B60H1/32 ,  C12Q1/02 ,  B60H3/00

CPC分类号： C12N11/00 ,  C12Q1/02 ,  A61L2/232 ,  B60H1/3227 ,  B60H3/0092

摘要： The present disclosure relates to a method for pretreating an evaporator for accelerating odor reproduction of an odor reproduction device for a vehicle air conditioning system, and a method for pretreating an evaporator surface of the present disclosure may include: a step of preparing an inoculation of malodorous microorganisms; a step of preparing and pretreating an evaporator; and a step of immobilizing malodorous microorganisms on an evaporator surface.

公开(公告)号：US11560014B2

公开(公告)日：2023-01-24

申请号：US16692744

申请日：2019-11-22

申请人： Brown University

发明人： Thomas J. Webster

IPC分类号： B44C1/22 ,  C12N1/36 ,  C12N11/00 ,  A61M16/04 ,  C23F1/14

摘要： The present invention is directed to methods for inhibiting growth of bacteria and to nanometer scale surfaces having antibacterial properties.

公开(公告)号：US20210395707A9

公开(公告)日：2021-12-23

申请号：US16914195

申请日：2020-06-26

申请人： Quantum-Si Incorporated

发明人： Brian Reed ,  Manjula Pandey

IPC分类号： C12N9/12 ,  C12N11/00 ,  C12Q1/6806 ,  C12P19/34

摘要： Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US11015163B2

公开(公告)日：2021-05-25

申请号：US16028913

申请日：2018-07-06

申请人： T&R BIOFAB CO., LTD. ,  Korea Polytechnic University Industry Academic Cooperation Foundation

发明人： Geunseon Ahn ,  Jinhyung Shim ,  Kyunghyun Min ,  Wonsoo Yun ,  Songwan Jin

IPC分类号： C12M1/02 ,  B33Y30/00 ,  C12N11/00 ,  B29C64/30 ,  C12M1/26 ,  B29C64/364 ,  B33Y40/00 ,  C12M3/00 ,  B29C64/106 ,  B29C64/295 ,  B33Y10/00

摘要： A cell printing apparatus according to the present disclosure comprises: a nozzle through which a liquid cell substance is discharged; a container in which the liquid cell substance discharged through the nozzle is laminated into a three-dimensional structure; and a supply unit for supplying the liquid heated to a predetermined temperature to the container.

公开(公告)号：US10881781B1

公开(公告)日：2021-01-05

申请号：US16862378

申请日：2020-04-29

申请人： ORTH CONSULTING, LLC

发明人： Donald S. Orth

IPC分类号： A61M1/36 ,  A61M1/26 ,  C12N11/00 ,  B01J31/00

摘要： A detoxification method includes the steps of inducing flow of patient blood through an extracorporeal device inlet and outlet in fluid connection to the circulatory system of a patient. Biological agents including lipopolysaccharide (LPS) contained within patient blood can be detoxified by passing patient blood over a biochemical reactor surface having attached or immobilized Saccharomyces boulardii alkaline phosphatase enzyme, with the biochemical reactor being contained within the extracorporeal device.

公开(公告)号：US20200248163A1

公开(公告)日：2020-08-06

申请号：US16648919

申请日：2018-09-28

申请人： Enzymaster (Ningbo) Bio-Engineering Co., Ltd.

发明人： Haibin CHEN ,  Yong Koy BONG ,  Wenhui ZHENG ,  Zhaoqi ZHANG ,  Kuifang HE ,  Chengxiao ZHANG ,  Lei SUN ,  Yingxin ZHANG

IPC分类号： C12N9/88 ,  C12N11/00 ,  C12P13/06

摘要： The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce β-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing β-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or β-alanine. The use of the engineered polypeptides of the present invention for the preparation of β-alanine results in higher unit activity, lower cost, and has good industrial application prospects.

公开(公告)号：US20200238751A1

公开(公告)日：2020-07-30

申请号：US16692744

申请日：2019-11-22

申请人： Brown University

发明人： Thomas J. Webster

IPC分类号： B44C1/22 ,  C23F1/14 ,  A61M16/04 ,  C12N11/00 ,  C12N1/36

摘要： The present invention is directed to methods for inhibiting growth of bacteria and to nanometer scale surfaces having antibacterial properties.