- 专利标题: BACILLUS SUBTILIS GENETICALLY ENGINEERED BACTERIUM FOR PRODUCING TAGATOSE AND METHOD FOR PREPARING TAGATOSE
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申请号: US18260333申请日: 2021-07-28
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公开(公告)号: US20240254527A1公开(公告)日: 2024-08-01
- 发明人: Yanhe MA , Ting SHI , Yunjie LI , Pingping HAN , Yuan LI
- 申请人: TIANJIN YEAHE BIOTECHNOLOGY CO., LTD
- 申请人地址: CN Tianjin
- 专利权人: TIANJIN YEAHE BIOTECHNOLOGY CO., LTD
- 当前专利权人: TIANJIN YEAHE BIOTECHNOLOGY CO., LTD
- 当前专利权人地址: CN Tianjin
- 优先权: CN 2110006046.4 2021.01.05
- 国际申请: PCT/CN2021/108983 2021.07.28
- 进入国家日期: 2023-07-04
- 主分类号: C12P19/02
- IPC分类号: C12P19/02 ; C12N9/10 ; C12N9/16 ; C12N9/90 ; C12N11/00 ; C12N15/75
摘要:
Provided are a Bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.
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