公开(公告)号：US20240254527A1

公开(公告)日：2024-08-01

申请号：US18260333

申请日：2021-07-28

申请人： TIANJIN YEAHE BIOTECHNOLOGY CO., LTD

发明人： Yanhe MA ,  Ting SHI ,  Yunjie LI ,  Pingping HAN ,  Yuan LI

IPC分类号： C12P19/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/90 ,  C12N11/00 ,  C12N15/75

CPC分类号： C12P19/02 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/90 ,  C12N11/00 ,  C12N15/75 ,  C12Y204/01001 ,  C12Y301/03 ,  C12Y501/03 ,  C12Y503/01009 ,  C12Y504/02002

摘要： Provided are a Bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.

公开(公告)号：US20240191221A1

公开(公告)日：2024-06-13

申请号：US18554463

申请日：2022-02-11

申请人： TIANJIN YEAHE BIOTECHNOLOGY CO., LTD

发明人： Yanhe MA ,  Ting SHI ,  Pingping HAN ,  Yunjie LI

IPC分类号： C12N11/08 ,  C12N9/12 ,  C12N9/90 ,  C12N11/18 ,  C12N15/52 ,  C12N15/74 ,  C12P19/02

CPC分类号： C12N11/08 ,  C12N9/1205 ,  C12N9/90 ,  C12N11/18 ,  C12N15/52 ,  C12N15/74 ,  C12P19/02 ,  C12Y207/01144 ,  C12Y501/03 ,  C12Y503/01009 ,  C12Y504/02002

摘要： Provided are the immobilization of multiple enzymes on the basis of an artificial oil body and an application thereof in the preparation of tagatose. Specifically, an artificial oil body is used to mix an expressed fusion protein of target protease-oil body protein with an oil body, which then undergoes an ultrasonic treatment; the fusion protein is anchored to the surface of the oil body by means of the specific hydrophobicity of a human protein to form an artificial oil body containing the target protease, so that the purification and immobilization of enzymes can be completed simultaneously. The immobilized multiple enzymes that can be used for tagatose production utilize an artificial oil body as an immobilized enzyme substrate, which significantly improves the stability of the immobilized enzymes, reduces the production cost of the current enzymatic preparation of tagatose, and has a simple preparation process.