公开(公告)号：US20240352440A1

公开(公告)日：2024-10-24

申请号：US18641530

申请日：2024-04-22

申请人： BONUMOSE, INC.

发明人： Daniel Joseph WICHELECKI ,  Edwin O. ROGERS

IPC分类号： C12N9/90 ,  C12N9/10 ,  C12N9/16 ,  C12N9/92 ,  C12P19/02 ,  C12P19/14 ,  C12P19/18 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/92 ,  C12P19/02 ,  C12P19/14 ,  C12P19/18 ,  C12P19/24 ,  C12Y204/01001 ,  C12Y301/03011 ,  C12Y503/01009 ,  C12Y504/02002

摘要： Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

公开(公告)号：US20240306693A1

公开(公告)日：2024-09-19

申请号：US18263830

申请日：2022-02-02

申请人： BONUMOSE, INC.

发明人： Daniel Joseph WICHELECKI ,  Edwin O. ROGERS

IPC分类号： A23L33/20 ,  A21D2/26 ,  A23L33/17 ,  C12N9/12 ,  C12N9/90 ,  C12N9/92

CPC分类号： A23L33/20 ,  A21D2/26 ,  A23L33/17 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12Y207/01144 ,  C12Y401/02022 ,  C12Y503/01009 ,  C12Y504/02002

摘要： The invention relates to preparation of food ingredients enriched with a low-glycemic sugar replacement through enzymatic conversion. Food ingredients may be enriched with, for example, D-tagatose, D-allulose, D-allose, D-mannose, D-talose, and/or inositol by enzymatically converting saccharides found in flour, meal, ground tuber, ground pulse, ground bark, starch, malted grain or malt extract, maltodextrin, cellulose, cellodextrin, any of their derivatives (e.g., amylose, amylopectin, dextrin, cellobiose, etc.), and/or sucrose into D-tagatose, D-allulose, D-allose, D-mannose, D-talose and/or inositol. The enriched material can be used as a food ingredient instead of the low-glycemic sugar being purified for use as a food ingredient.

公开(公告)号：US20240093258A1

公开(公告)日：2024-03-21

申请号：US18038067

申请日：2021-11-24

申请人： Manus Bio Inc.

发明人： Ajikumar PARAYIL KUMARAN ,  Christine Nicole S. SANTOS ,  Jason DONALD ,  Aaron LOVE ,  Yiying ZHENG ,  Adel GHADERI ,  Vineet SHASTRY ,  Lu CHEN ,  Christopher TOOMEY ,  Hannah LYNCH ,  Eric NIEMINEN

IPC分类号： C12P19/60 ,  C12N9/10 ,  C12N9/90

CPC分类号： C12P19/60 ,  C12N9/1051 ,  C12N9/90 ,  C12Y204/01017 ,  C12Y503/01009

摘要： In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.

公开(公告)号：US20180201958A1

公开(公告)日：2018-07-19

申请号：US15744049

申请日：2016-07-21

申请人： The Regents of the University of California

发明人： James U. Bowie ,  Tyler P. Korman ,  Paul H. Opgenorth

IPC分类号： C12P7/16 ,  C12P7/62

CPC分类号： C12P7/16 ,  C12P7/625 ,  C12Y101/01044 ,  C12Y202/01001 ,  C12Y202/01002 ,  C12Y203/01008 ,  C12Y301/01031 ,  C12Y401/02009 ,  C12Y401/02013 ,  C12Y401/02022 ,  C12Y501/03001 ,  C12Y503/01001 ,  C12Y503/01006 ,  C12Y503/01009 ,  Y02E50/10

摘要： Provided is an engineered pathway that can function in a cell-free system, cellular system or a combination thereof to convert a sugar to a chemical or biofuel.

公开(公告)号：US09796991B2

公开(公告)日：2017-10-24

申请号：US14891622

申请日：2015-02-04

申请人： INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES

发明人： Tingyi Wen ,  Xiuling Shang ,  Yun Zhang ,  Shuwen Liu ,  Yong Liang ,  Yu Zhang

IPC分类号： C12P13/04 ,  C12P13/24 ,  C12N9/04 ,  C12N9/92 ,  C12N15/77

CPC分类号： C12P13/24 ,  C12N9/0006 ,  C12N9/92 ,  C12N15/77 ,  C12Y101/01043 ,  C12Y101/01049 ,  C12Y503/01009

摘要： The present invention relates to recombinant bacteria producing L-amino acid, in which the recombinant bacteria has reduced expression of the glucose-6-phosphate isomerase gene pgi and improved expression of the glucose-6-phosphate dehydrogenase gene -opcA than the starting bacteria, where the starting bacterium is a bacterial strain that can accumulate target amino acid(s) and preferably, the amino acid is L-histidine.

公开(公告)号：US09376695B2

公开(公告)日：2016-06-28

申请号：US12238704

申请日：2008-09-26

申请人： Rustem Saidovich Shakulov ,  Elena Vitalievna Klyachko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh

发明人： Rustem Saidovich Shakulov ,  Elena Vitalievna Klyachko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh

IPC分类号： C12P13/22 ,  C12P13/20 ,  C12P13/24 ,  C12P21/02 ,  C12N9/02 ,  C12N9/90 ,  C12N9/92

CPC分类号： C12P21/02 ,  C12N9/001 ,  C12N9/90 ,  C12N9/92 ,  C12P13/20 ,  C12P13/222 ,  C12P13/24 ,  C12Y103/01013 ,  C12Y503/01009 ,  C12Y504/99005 ,  Y02P20/52

摘要： A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.

摘要翻译： 通过使用肠杆菌科的细菌进行发酵，描述了L-氨基酸的制造方法，例如L-苯丙氨酸和L-组氨酸，其中，通过将能够转录的DNA片段连接到编码 SEQ ID NO：2所示的肽，或其变体，特别是ssrA基因的一部分，对编码细菌酶的基因的3'末端，其影响L-氨基酸生物合成，例如绒毛膜变位酶 / prephenate脱氢酶或磷酸葡萄糖异构酶。

公开(公告)号：US20230313208A1

公开(公告)日：2023-10-05

申请号：US18016432

申请日：2021-07-14

申请人： Academia Sinica ,  The Regents of the University of California

发明人： Yu-Hsiao Chen ,  Hsin-Wei Jung ,  Chao-Yin Tsuei ,  James C. Liao

IPC分类号： C12N15/70 ,  C12N9/04 ,  C12N9/88 ,  C12N9/92 ,  C12N1/20 ,  C12N1/32 ,  C12P7/06 ,  C12P7/16 ,  C12P7/46

CPC分类号： C12N15/70 ,  C12N1/205 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/88 ,  C12N9/92 ,  C12P7/065 ,  C12P7/16 ,  C12P7/46 ,  C12R2001/19 ,  C12Y101/01244 ,  C12Y401/02043 ,  C12Y503/01006 ,  C12Y503/01009 ,  C12Y503/01027

摘要： Provided herein are metabolically-modified microorganisms that can grow on an organic C1 carbon source.

公开(公告)号：US10017802B2

公开(公告)日：2018-07-10

申请号：US15045704

申请日：2016-02-17

申请人： FIDIA FARMACEUTICI S.P.A.

发明人： Vincenza Corsa ,  Alessandro Negro ,  Susanna Vaccaro ,  Luciano Messina

IPC分类号： C12P19/26 ,  C08B37/08 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/92 ,  C12N15/75 ,  C12N1/20 ,  C12N15/63

CPC分类号： C12P19/26 ,  C08B37/0072 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/635 ,  C12N15/75 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

摘要： The present invention relates to a method for the production of hyaluronic acid (HA) in Bacillus subtilis and Escherichia coli through plasmid vectors wherein the gene is under the control of strong promoter Pgrac, and a system for the selection of stable bacterial strains for the production of high levels of hyaluronic acid.

公开(公告)号：US20180171032A1

公开(公告)日：2018-06-21

申请号：US15737434

申请日：2016-06-17

申请人： Fundación IMDEA Materiales

发明人： Rubén Dario COSTA RIQUELME ,  Uwe SONNEWALD ,  Pedro BRANA COTO ,  Michael Dominik WEBER ,  Marlene PRÖSCHEL

IPC分类号： C07K17/04 ,  C12N9/96 ,  C07K17/08 ,  C08J3/075 ,  C09K11/06 ,  C09K11/02 ,  C12Q1/00 ,  C12Q1/40 ,  C12Q1/533 ,  C12Q1/48 ,  C12N9/12 ,  C12N9/26 ,  C12N9/92 ,  H01L33/56

CPC分类号： C07K17/04 ,  B82Y5/00 ,  C07K17/08 ,  C08G2650/30 ,  C08J3/075 ,  C08J2371/02 ,  C08J2471/02 ,  C08J2489/00 ,  C09D171/02 ,  C09K11/025 ,  C09K11/06 ,  C09K2211/14 ,  C12N9/1205 ,  C12N9/2431 ,  C12N9/92 ,  C12N9/96 ,  C12Q1/008 ,  C12Q1/40 ,  C12Q1/485 ,  C12Q1/533 ,  C12Y207/01001 ,  C12Y503/01009 ,  H01L33/56

摘要： The present invention relates to a process of preparing a rubber-like material containing a protein immobilized therein, as well as a corresponding rubber-like material, the process comprising the steps of (a) mixing a protein, a branched polymer such as trimethylolpropane ethoxylate and a linear polymer such as poly(ethylene oxide) in an aqueous solution to form a gel, and (b) drying the gel to obtain a rubber-like material containing the protein immobilized therein, wherein the branched polymer comprises at least three polymeric branches bound to a central branching unit. The rubber-like material allows the immobilization and stabilization of a wide range of different proteins, including luminescent proteins as well as enzymes, and can particularly advantageously be used as down-converting material for light-emitting diodes (LEDs), for diagnostic applications, and in bioreactors.

公开(公告)号：US09708637B2

公开(公告)日：2017-07-18

申请号：US15164381

申请日：2016-05-25

申请人： AJINOMOTO CO., INC.

发明人： Rustem Saidovich Shakulov ,  Elena Vitalievna Klyachko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh

IPC分类号： C12P13/24 ,  C12P21/02 ,  C12P13/20 ,  C12P13/22 ,  C12N9/02 ,  C12N9/90 ,  C12N9/92

CPC分类号： C12P21/02 ,  C12N9/001 ,  C12N9/90 ,  C12N9/92 ,  C12P13/20 ,  C12P13/222 ,  C12P13/24 ,  C12Y103/01013 ,  C12Y503/01009 ,  C12Y504/99005 ,  Y02P20/52

摘要： A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.