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    NOVEL GLYCOSYLTRANSFERASE GENE AND USE THEREOF
    7.
    发明申请
    NOVEL GLYCOSYLTRANSFERASE GENE AND USE THEREOF 审中-公开
    新型GLYCOSYLTRANSFERASE基因及其用途

    公开(公告)号：US20170058269A1

    公开(公告)日：2017-03-02

    申请号：US15308117

    申请日：2015-05-01

    申请人： SUNTORY HOLDINGS LIMITED

    发明人： Yoshikazu TANAKA ,  Naoko OKITSU ,  Keisuke MATSUI

    IPC分类号： C12N9/10 ,  C12P17/06

    CPC分类号： C12N9/1048 ,  A01H5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12P17/06 ,  C12P19/60 ,  C12P21/00 ,  C12Y204/00

    摘要： Provided is a polynucleotide for encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4′-glucoside. A polynucleotide selected from the group consisting of: (a) polynucleotides comprising a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5; (b) polynucleotides that hybridize, under stringent conditions, with polynucleotides comprising a base sequence complementary to a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5, wherein the polynucleotides encode a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; (c) polynucleotides for encoding proteins comprising an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6; (d) polynucleotides for encoding proteins comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6, the polynucleotides exhibiting activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; and the like.

    摘要翻译： 本发明提供一种编码蛋白质的多核苷酸，其表现出将糖转移到黄酮，特别是黄酮4'-葡萄糖苷的第7位的羟基的活性。 选自以下的多核苷酸：（a）包含SEQ ID NO：1或SEQ ID NO：5的碱基序列的多核苷酸; （b）在严格条件下与包含与SEQ ID NO：1或SEQ ID NO：5的碱基序列互补的碱基序列的多核苷酸杂交的多核苷酸，其中所述多核苷酸编码显示转移糖的活性的蛋白质 到黄酮的第7位的羟基; （c）编码包含SEQ ID NO：2或SEQ ID NO：6的氨基酸序列的蛋白质的多核苷酸; （d）用于编码蛋白质的多核苷酸，其包含其中一个或多个氨基酸已被缺失，取代，插入和/或加入到SEQ ID NO：2或SEQ ID NO：6的氨基酸序列中的氨基酸序列 表现出将糖转移到黄酮第7位羟基的活性的多核苷酸; 等等。

    PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING AN HYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID
    8.
    发明申请
    PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING AN HYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID 有权
    生产包含水溶性2-O-ALPHA-D-葡萄糖-L-抗坏血酸的颗粒组合物的方法

    公开(公告)号：US20140348922A1

    公开(公告)日：2014-11-27

    申请号：US14295546

    申请日：2014-06-04

    申请人： Hayashibara Co., Ltd.

    发明人： Takashi SHIBUYA ,  Seisuke IZAWA ,  Tomoyuki NISHIMOTO ,  Shigeharu FUKUDA ,  Toshio MIYAKE

    IPC分类号： A61K31/7048 ,  A61K9/16

    CPC分类号： A61K31/7048 ,  A61K9/16 ,  C07H17/04 ,  C12P19/18 ,  C12P19/60 ,  C12Y204/01019

    摘要： The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside.

    摘要翻译： 本发明提供了一种能够生产含有抗坏血酸2-葡萄糖苷的无水结晶抗坏血酸2-葡萄糖苷的颗粒组合物的方法，即使当抗坏血酸2-葡萄糖苷的产率不达到35重量％时也不显着。 制备含有无水结晶抗坏血酸2-葡萄糖苷的颗粒组合物的方法，其包括使CGT酶作用于含有液化淀粉或糊精和L-抗坏血酸的溶液，然后使葡糖淀粉酶作用于所得溶液以获得 将抗坏血酸2-葡萄糖苷产率至少为27％的溶液，纯化得到的溶液，将抗坏血酸2-葡萄糖苷含量提高至86重量％以上，将无水结晶性抗坏血酸2-葡萄糖苷通过 控制冷却方式或假控制冷却方法，收集沉淀的无水结晶抗坏血酸2-葡萄糖苷，并对收集的无水结晶抗坏血酸2-葡萄糖苷进行老化和干燥。

    Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
    9.
    发明授权
    Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid 有权
    制备2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的方法

    公开(公告)号：US08759030B2

    公开(公告)日：2014-06-24

    申请号：US10523920

    申请日：2003-07-07

    申请人： Kazuhisa Mukai ,  Keiji Tsusaki ,  Michio Kubota ,  Shigeharu Fukuda ,  Toshio Miyake

    发明人： Kazuhisa Mukai ,  Keiji Tsusaki ,  Michio Kubota ,  Shigeharu Fukuda ,  Toshio Miyake

    IPC分类号： C12P19/44

    CPC分类号： C12P19/60

    摘要： The object of the present invention is to provide a method and a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid where 5-O-α-glucopyranosyl-L-ascorbic acid and 6-O-α-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid comprising the steps of allowing α-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an α-glucosyl saccharide to form 2-O-α-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-α-glucopyranosyl-L-ascorbic acid.

    摘要翻译： 本发明的目的是提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法和方法，其中5-O-α-吡喃葡萄糖基-L-抗坏血酸和6-O-α-吡喃葡萄糖基 - L-抗坏血酸不是以这样的少量形成或形成，也可以不形成。 本发明通过提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法来解决上述目的，其包括以下步骤：将α-异麦芽糖基葡萄糖形成酶与或不与环糊精葡聚糖转移酶（EC 2.4.1.19）一起 作用于包含L-抗坏血酸和α-葡萄糖基糖的溶液中以形成2-O-α-吡喃葡萄糖基-L-抗坏血酸并收集形成的2-O-α-吡喃葡萄糖基-L-抗坏血酸。

    Glycosyltransferase, novel glycosyltransferase gene, and novel sugar donor compound
    10.
    发明授权
    Glycosyltransferase, novel glycosyltransferase gene, and novel sugar donor compound 有权
    糖基转移酶，新型糖基转移酶基因和新型糖供体化合物

    公开(公告)号：US08722868B2

    公开(公告)日：2014-05-13

    申请号：US13389233

    申请日：2010-01-21

    申请人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

    发明人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

    IPC分类号： C07H21/04 ,  C12N9/10 ,  C12N15/00 ,  C12N5/10

    CPC分类号： C12N9/1048 ,  C07H13/08 ,  C07H15/18 ,  C07H17/065 ,  C12P19/44 ,  C12P19/60

    摘要： An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein R1 is independently selected from hydrogen, or C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO2, and SO2, n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

    摘要翻译： 本发明的目的是提供一种含糖糖核苷酸以外的糖供体化合物和能够使用糖核苷酸以外的糖供体化合物催化糖基转移反应的酶的供糖试剂。 本发明提供以下：含有式（A）化合物的给糖试剂：其中R 1独立地选自氢或C 1-6烷基，C 2-6烯基和C 2-6炔基，其中每个基团 未取代或被一个或多个选自OH，F，Cl，Br，I，CN，NO2和SO2的基团取代，n为0,1,2,3,4或5，m为0或1，X 代表通过＆bgr结合的单糖; 其端基异碳碳键; 能够使用糖供体催化糖基转移反应的糖基转移酶; 和包含编码糖基转移酶的DNA的糖基转移酶基因。