POLYVINYL ALCOHOL-DEGRADING ENZYME AND PROCESS FOR PRODUCING THE SAME

    公开(公告)号:US20210207187A1

    公开(公告)日:2021-07-08

    申请号:US16490000

    申请日:2018-02-23

    IPC分类号: C12P21/02 C12N15/11 C12N9/14

    摘要: The objects of the present invention are to provide a novel PVA-degrading enzyme that the entity of which is revealed at the amino acid sequence level, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, and a transformant having the recombinant DNA. The present invention solves the above objects by providing a polyvinyl alcohol-degrading enzyme having the following characteristics (1) to (3), a process for producing the same, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, and a transformant having the recombinant DNA: (1) having an activity of oxidizing polyvinyl alcohol and forming hydrogen peroxide; (2) having an activity of hydrolyzing β-diketone, and (3) exhibiting a molecular weight of 100,000±20,000 in SDS-polyacrylamide gel electrophoresis.

    PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING AN HYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID
    4.
    发明申请
    PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING AN HYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID 有权
    生产包含水溶性2-O-ALPHA-D-葡萄糖-L-抗坏血酸的颗粒组合物的方法

    公开(公告)号:US20160024131A1

    公开(公告)日:2016-01-28

    申请号:US14879280

    申请日:2015-10-09

    IPC分类号: C07H17/04

    摘要: The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside.

    摘要翻译: 本发明提供了一种能够生产含有抗坏血酸2-葡萄糖苷的无水结晶抗坏血酸2-葡萄糖苷的颗粒组合物的方法,即使当抗坏血酸2-葡萄糖苷的产率不达到35重量%时也不显着。 制备含有无水结晶抗坏血酸2-葡萄糖苷的颗粒组合物的方法,其包括使CGT酶作用于含有液化淀粉或糊精和L-抗坏血酸的溶液,然后使葡糖淀粉酶作用于所得溶液以获得 将抗坏血酸2-葡萄糖苷产率至少为27%的溶液,纯化得到的溶液,将抗坏血酸2-葡萄糖苷含量提高至86重量%以上,将无水结晶性抗坏血酸2-葡萄糖苷通过 控制冷却方式或假控制冷却方法,收集沉淀的无水结晶抗坏血酸2-葡萄糖苷,并对收集的无水结晶抗坏血酸2-葡萄糖苷进行老化和干燥。

    PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING AN HYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID
    7.
    发明申请
    PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING AN HYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID 有权
    生产包含水溶性2-O-ALPHA-D-葡萄糖-L-抗坏血酸的颗粒组合物的方法

    公开(公告)号:US20140348922A1

    公开(公告)日:2014-11-27

    申请号:US14295546

    申请日:2014-06-04

    IPC分类号: A61K31/7048 A61K9/16

    摘要: The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside.

    摘要翻译: 本发明提供了一种能够生产含有抗坏血酸2-葡萄糖苷的无水结晶抗坏血酸2-葡萄糖苷的颗粒组合物的方法,即使当抗坏血酸2-葡萄糖苷的产率不达到35重量%时也不显着。 制备含有无水结晶抗坏血酸2-葡萄糖苷的颗粒组合物的方法,其包括使CGT酶作用于含有液化淀粉或糊精和L-抗坏血酸的溶液,然后使葡糖淀粉酶作用于所得溶液以获得 将抗坏血酸2-葡萄糖苷产率至少为27%的溶液,纯化得到的溶液,将抗坏血酸2-葡萄糖苷含量提高至86重量%以上,将无水结晶性抗坏血酸2-葡萄糖苷通过 控制冷却方式或假控制冷却方法,收集沉淀的无水结晶抗坏血酸2-葡萄糖苷,并对收集的无水结晶抗坏血酸2-葡萄糖苷进行老化和干燥。